The invention discloses an acetate fiber monoazo dye mixture. According to the total mass of the dye mixture, the acetate fiber monoazo dye mixture comprises the following active ingredients in percentage by weight: 10-40% of compound A, 5-30% of compound B, 0.5-50% of compound C, and the balance of dispersing agent, or 10-40% of compound A, 3-30% of compound D, 1-40% of compound E, and the balance of dispersing agent, or 2-35% of compound A, 5-25% of compound B, 0.1-10% of compound C, 4-20% of compound D, 5-30% of compound E, and the balance of dispersing agent. The acetate fiber monoazo dye mixture is reasonable in formula, excellent in dying performance, wide in applications, high in the added value of the product, and can be used for dying acetate fibers under the low-temperature condition. The invention further discloses a preparation method of the acetate fiber monoazo dye mixture, which comprises the following steps of: mixing all the active ingredients according to a proportion, adding water and grinding, and conducting spray drying, and the preparation method is simple in technology steps, strong in operability, low in cost, and suitable for industrial production.