Chlorotoluron pesticide residue degradation strain agent prepared by the strain

A technology for the degradation of pesticide residues and inoculants, which is applied in microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of limited use and narrow degradation spectrum of strains, and achieve increased added value, low production and use costs, and good removal effect. Effect

Inactive Publication Date: 2009-01-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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  • Chlorotoluron pesticide residue degradation strain agent prepared by the strain

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Embodiment Construction

[0013] Examples of the present invention are described below.

[0014] The invention provides a degrading bacterium that eliminates chlorotoluron pesticide residues. Its strain YBL3 (preserved in the China Center for Type Culture Collection on May 22, 2008, with a strain preservation number of CCTCC NO: M 208076) is a strain isolated by the patent applicant. The soil samples collected from the pesticide factory belonged to Gram-negative bacteria and were identified as Sphingobium sp. The main biological characteristics are negative Gram reaction, the bacteria are straight rods without spores, the size of the bacteria is 0.6-0.7×1.5-1.6μm, non-fermenting lactose is positive, V-P reaction is negative, and methyl red reaction is negative , does not produce indole, negative for hydrogen sulfide, positive for nitrate reduction. The Genbank accession number of the strain 16S rDNA is EU159273.

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Abstract

The invention provides a pesticide-degrading bacterium for removing the residue of a herbicide of chlorotoluron; the bacterium that is used is gram staining reaction negative bacilli YBL3 which is identified to be Sphingobium sp. The bacterium is preserved in China Center for Type Culture Collection in May 23rd, 2008 and the preservation number of the bacterium is CCTCC M208076. The technique for producing the bacterium includes: slant seed-shake flask seed-seed pot-production pot-product (the packaging formulation is liquid bacterium or solid absorption bacterium). The product of the degrading bacterium can be directly applied to reduce the pesticide residue in the crops by more than 90 percent, thus solving the over standard problem of the pesticide residue in agricultural production and being capable of producing green agricultural products with no poison and social effects of pollution.

Description

1. Technical field [0001] The invention discloses a chlorotoluron herbicide residue-degrading bacteria and the bacterial agent produced therefrom, which belong to the field of biotechnology, degrade chemical pesticide residues by using microorganisms, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Background technology [0002] The use of herbicides has greatly promoted the development of modern agriculture, saved a lot of losses caused by weeds or residual pesticide damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemicals. When they enter the ecological environment, some herbicides remain in the soil for a long time, and the residual pesticides are sensitive to subsequent crops and cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04
Inventor 李顺鹏孙纪全黄星梁斌何健赵煜坤
Owner NANJING AGRICULTURAL UNIVERSITY
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