Rapid detection method of coliform groups

A coliform and detection method technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral characteristic measurement, etc., can solve problems that affect application, guide production, and long detection time

Inactive Publication Date: 2010-12-15
殷涌光
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  • Application Information

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Problems solved by technology

At present, the commonly used detection method of coliform group is the multi-tube fermentation method. The detection result of this method is accurate and reliable, but because of its long detection time (72h), it affects its application in food and water quality monitoring, especially In the case of a large number of testing samples and tig...

Method used

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  • Rapid detection method of coliform groups
  • Rapid detection method of coliform groups
  • Rapid detection method of coliform groups

Examples

Experimental program
Comparison scheme
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Embodiment

[0043] 1. Determine the number of coliform bacteria in the culture medium by counting the number of cells in the pellet.

[0044] 1), preparation of mixed culture solution of eosin-methylene blue

[0045] Prepare 3 kinds of eosin-methylene mixed culture solutions, which are:

[0046] a. According to the ratio of 22.5g lactic acid bile salt medium powder: 409mg eosin: 66mg methylene blue: 500mL distilled water, dissolve it in distilled water, add lactic acid according to the ratio of 1 mL of solution: 30 μL 1% lactic acid solution, and prepare eosin methylene blue Mixed culture solution, eosin melanin mixed culture solution is neutral, pH is 6.95-7.07. Boil on an electric stove, seal with tin foil, and sterilize for later use;

[0047] b. According to the ratio of 22.5g lactic acid bile salt medium powder: 550mg eosin: 89mg methylene blue: 500mL distilled water, dissolve in distilled water, add lactic acid according to the ratio of 1mL of solution: 30μL 1% lactic acid solutio...

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Abstract

The invention relates to a rapid detection method of coliform groups. In the method, the situation that coliform group cells can generate gas and ferment lactose to generate acid in a short-time culture process is utilized to combine the coliform group cells with eosin methylene blue to ensure that a mixed eosin methylene blue culture solution generates atropurpureus or purplish red precipitation, and the image number of negative bacteria is obtained after carrying out gram stain on the atropurpureus or purplish red precipitation through a biological microscope lens so as to count the number of the coliform groups, or the mixed eosin methylene blue culture solution changes colors because of the atropurpureus or purplish red precipitation generated by the mixed eosin methylene blue culture solution, and the cell number of the coliform groups is recognized by utilizing the characteristics that the color change degree of the mixed eosin methylene blue culture solution is related to the cell number of the coliform groups. The method is suitable to rapid detection of the coliform groups in the fields of biology, medicines, foods and various articles.

Description

technical field [0001] The invention relates to a rapid detection method for coliform bacteria. The invention relates to a kind of purple-black color produced by coliform cells that can produce gas in a short-term culture process, can ferment lactose to produce acid, and combine coliform cells with eosin methylene blue to form eosin methylene blue mixed culture solution (or purplish red) precipitation, count the number of images of bacteria with purple black (or purple red) precipitated bacteria negative through Gram staining to count the coliform rapid detection method for the number of coliforms through biological microscope examination; Or due to the combination of coliform cells and eosin methylene blue, the purple-black precipitate generated by the eosin methylene mixed culture solution will change the color of the eosin methylene mixed culture solution, and the color change of the eosin methylene mixed culture solution can be used The degree is related to the number of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/10C12Q1/06G01N21/31G01N21/78
Inventor 殷涌光
Owner 殷涌光
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