Triethylamine degrading bacterium and microbial agent produced therefrom

A technology of triethylamine and degrading bacteria, which is applied in the high biological field, can solve the problems of secondary pollution and high cost, and achieve the effect of reducing wastewater treatment cost, low production cost and good removal effect

Active Publication Date: 2011-01-05
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Triethylamine is widely present in organic synthesis, pesticides, dyes and other production wastewater. At present, physical and chemical methods are mostly used for the treatment of triethylamine-

Method used

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  • Triethylamine degrading bacterium and microbial agent produced therefrom
  • Triethylamine degrading bacterium and microbial agent produced therefrom
  • Triethylamine degrading bacterium and microbial agent produced therefrom

Examples

Experimental program
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Embodiment Construction

[0022] 1 Isolation and identification of strains

[0023] Take the triethylamine-enriched bacterial solution and perform gradient dilution. The dilution is coated on the inorganic salt solid medium containing 100mg / L triethylamine (the formula is: every liter contains 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 1.0g NaCl, 1.0g (NH 4 ) 2 SO 4 , 15g agar, pH 7.0), cultured at 30°C for several days. Pick a single colony to verify its degradation effect, and save a strain with higher degradation efficiency for subsequent experiments. See the picture of its colony figure 1 , identified as Arthrobacter protophormiae; named: R4. The main biological characteristic is G + , The logarithmic growth phase is rod-shaped, without spores, and grows aerobically. It can grow with triethylamine as the only carbon source and nitrogen source, and completely mineralize it to produce ammonia nitrogen. Under laboratory shaking flask condition, the degradation rate to 100mg / L...

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Abstract

The invention provides a degrading bacterium eliminating the pollution of triethylamine and a degrading microbial agent and belongs to the high-technical field of biology. The used strain is a gram staining reaction positive bacterium R4, is determined as arthrobacter protophormiae, and has a biological characteristic of G+. The logarithmic phase thallus is bacilliform, sporeless and aerobic to grow, and can grow by taking the triethylamine as the only carbon source or nitrogen source and thoroughly mineralize the triethylamine to generate ammonia nitrogen. In a flask experiment in a laboratory, the strain can completely degrade 100mg/L triethylamine and solve the problem of the difficult biodegradation of the triethylamine in wastewater treatment.

Description

1. Technical field [0001] The invention provides a triethylamine-degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade pollutants in waste water, and are suitable for modern waste water treatment processes. 2. Technical background [0002] Organic amine products are widely used in pesticides, pharmaceuticals, rubber, synthetic dyes, petroleum, leather, detergents, organic synthesis, inorganic chemicals and other industries. There are many types of organic amine products, and each product will have residual raw materials and intermediate products entering the wastewater during the production process. Most of these substances are high in nitrogen and low in carbon, toxic and harmful, and difficult to biodegrade. posed difficulties. [0003] Triethylamine is an important raw material for organic solvents and chemical synthesis. In the manufacture of synthetic resins, it is used as a catalyst ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/38
Inventor 蔡天明陈立伟任倩张金
Owner NANJING AGRICULTURAL UNIVERSITY
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