Triethylamine degrading bacterium and microbial agent produced therefrom
A technology of triethylamine and degrading bacteria, which is applied in the high biological field, can solve the problems of secondary pollution and high cost, and achieve the effect of reducing wastewater treatment cost, low production cost and good removal effect
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[0022] 1 Isolation and identification of strains
[0023] Take the triethylamine-enriched bacterial solution and perform gradient dilution. The dilution is coated on the inorganic salt solid medium containing 100mg / L triethylamine (the formula is: every liter contains 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 ·7H 2 O, 1.0g NaCl, 1.0g (NH 4 ) 2 SO 4 , 15g agar, pH 7.0), cultured at 30°C for several days. Pick a single colony to verify its degradation effect, and save a strain with higher degradation efficiency for subsequent experiments. See the picture of its colony figure 1 , identified as Arthrobacter protophormiae; named: R4. The main biological characteristic is G + , The logarithmic growth phase is rod-shaped, without spores, and grows aerobically. It can grow with triethylamine as the only carbon source and nitrogen source, and completely mineralize it to produce ammonia nitrogen. Under laboratory shaking flask condition, the degradation rate to 100mg / L...
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