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Paracoccus versutus strain and application thereof

A paracoccus and strain technology, applied in bacteria, gas fuels, biochemical equipment and methods, etc., can solve the problems of high economic cost, reducing methane concentration, and difficulty in oxygen regulation, and achieve the effect of avoiding potential safety hazards.

Active Publication Date: 2017-06-13
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photoautotrophic microorganisms remove H 2 S process, although a higher H 2 S removal rate and no safety hazards under anaerobic conditions, etc., but requires a large amount of radiant energy, its economic cost is high, and it is easily limited by the light source
Therefore, chemoautotrophic microorganisms become the biological removal of biogas H 2 A better choice for S. Under the condition of aerobic presence, chemoautotrophic microorganisms use oxygen as an electron acceptor, but the regulation of oxygen is difficult, and there is a danger of explosion when there is too much oxygen supply. At the same time, nitrogen and other gases will be introduced at the same time Inert gas, which reduces the concentration of methane in the biogas and reduces the energy efficiency of the biogas

Method used

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  • Paracoccus versutus strain and application thereof
  • Paracoccus versutus strain and application thereof
  • Paracoccus versutus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Isolation and purification of bacterial strains

[0032] The anaerobic sludge was taken from the third sewage treatment plant in Chengdu, Sichuan. The collected sludge was acclimatized with a medium enriched with sulfur compounds under anaerobic conditions, and then serially diluted after 7 days, starting from 10 -2 、10 -4 、10 -6 、10 -8 、10 -10 Draw 0.1ml of the dilution and spread it on the separation medium plate, and a single colony scattered on the surface of the plate appears, pick a single colony, repeat the above operation several times, and finally obtain a solution that can remove H from biogas using nitrate as an electron acceptor. 2 Purified strains of S.

[0033] The enrichment medium is: Na 2 S 2 o 3 ·5H 2 O 5g, KNO 3 4g, KH 2 PO 4 2g, NaHCO 3 1g, MgCl 2 ·6H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, pH value 7.0, tap water 1000mL. 121°C, 30min high temperature sterilization. Separation medium: Na 2 S 2 o 3 ·5H 2 O 10g, KNO 3 4g, KH 2 P...

Embodiment 2

[0045] The present invention paracoccus fimutans strain CGMCC No.13321 is applied to the H in biogas 2 S removed.

[0046] 1. Preparation of culture medium and inoculation solution

[0047] Solid activation medium: Na 2 S 2 o 3 ·5H 2 O 10g, KNO 3 4g, KH 2 PO 4 2g, NaHCO 3 1g, MgCl 2 ·6H 2 O0.5g, FeSO 4 ·7H 2 O 0.01g, 20g agar, 1000mL distilled water, pH7.0-7.5, 121℃, 30min high temperature sterilization. Liquid fermentation medium: Na 2 S 2 o 3 ·5H 2 O 10g, KNO 3 4g, KH 2 PO 4 2g, NaHCO 3 1g, 1000ml tap water, pH7.0-7.5, 121℃, 30min high temperature sterilization treatment. Desulfurization medium: KNO 3 4g, KH 2 PO 4 1g, NaHCO 3 1g, 1000ml tap water, pH7.0-7.5, 121℃, 30min high temperature sterilization treatment.

[0048] Inoculate Paracoccus mutans strain CGMCC No.13321 into the solid activation medium, and activate the culture for 1 day to obtain the activated strain, which is ready for use; put the activated strain into the liquid ferment...

Embodiment 3

[0055] Paracoccus mutans strain CGMCC No.13321 for biogas H 2 S removal, the same as Example 3 will not be repeated, the difference lies in the treatment conditions from the inoculated bacteria solution to the desulfurization reactor.

[0056] The treatment temperature is 20°C, and the H in the biogas is continuously measured 2 The removal rate of S is shown in Table 2 for the measurement results.

[0057] Table 2 Biogas H 2 S removal rate

[0058]

[0059] It can be seen from the above table that the temperature is 20°C, and the biogas H 2 The concentration of S was significantly lower than that of the control group, in which the biogas H 2 The removal rate of S was only 6.2%, while the experimental group biogas H 2 The removal rate of S is as high as 77.1%. Compared with the temperature condition of 30℃, although Paracoccus mutans strain CGMCC No.13321 2 The removal rate of S decreased, but still showed good H 2 S removal effect.

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Abstract

The invention discloses a paracoccus versutus (Paracoccus versutus) strain 2#CGMCC No. 13321 and application thereof for oxidizing nitrate serving as an electron acceptor to remove hydrogen sulfide (H2S) in biogas under anoxic conditions. The strain is subjected to Gram staining to be negative, and the strain cells are in a shape of short rods and are small; the bacterial colony is white and bulged, the edge is neat and smooth, and the surface is wet; the strain is not salt-tolerant, and citrate cannot be utilized; and in the presence of nitrate, nitrite or nitrogen oxide, anaerobic growth of the strain can be promoted by taking nitrate, nitrite and nitrogen oxide as electron acceptors. After the paracoccus versutus strain CGMCC No.13321 is inoculated to a desulfurization reactor, the H2S can be subjected to biological oxidation in the reactor by taking the nitrate as the electron receptor and then is subjected to growth and propagation, the aim of removing the H2S in the biogas is achieved, the potential safety hazard brought by mixing of the introduced methane and oxygen in the traditional method for removing the H2S in the biogas by taking the oxygen as the electron acceptor is effectively avoided, and the removal rate of the H2S in the biogas is up to 93.8%.

Description

technical field [0001] The present invention relates to a paracoccus bacterial strain, in particular to a method that can utilize nitrate as an electron acceptor to oxidize and remove hydrogen sulfide (H 2 S) Paracoccus mutans strain. Background technique [0002] Biogas is an emerging clean and renewable energy. Anaerobic fermentation technology with biogas as the target product has been more and more widely used because of its good energy and environmental benefits. It will be of great significance to alleviate my country's energy crisis and protect the environment. Biogas is a kind of biofuel produced by anaerobic fermentation of biological raw materials. The biological raw materials used for anaerobic fermentation of biogas mainly include livestock and poultry manure, straw, energy plants, domestic garbage, organic wastewater and other wastes. At present, the main raw materials for biogas fermentation in my country are manure and sewage from livestock and poultry farms....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10L3/10C12R1/01
CPCC10L3/103C12N1/20C12N1/205C12R2001/01Y02E50/30
Inventor 闫志英唐维林肖莉凡刘晓风袁月祥李东姬高升房俊楠
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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