A kind of bacillus pumilus and its application in killing nematodes

A kind of Bacillus pumilus, nematicide technology, applied in the direction of nematicide, application, bacteria, etc., to achieve the effect of good killing effect

Inactive Publication Date: 2011-12-21
HONGYUN HONGHE TOBACCO (GRP) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have not been any reports of Bacillus pumilus killing root-knot nematodes

Method used

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  • A kind of bacillus pumilus and its application in killing nematodes
  • A kind of bacillus pumilus and its application in killing nematodes
  • A kind of bacillus pumilus and its application in killing nematodes

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Bacillus pumilus ( Bacillus pumilus ) preparation

[0028] Bacillus pumilus ( Bacillus pumilus ) obtained from the wild, the specific cultivation method is as follows:

[0029] 1) Test tube culture: beef extract peptone medium: peptone 10g, beef extract 3g, sodium chloride 5g, agar 13g, distilled water 1000ml, pH6.8~7.2, inoculate Bacillus pumilus on the medium, 25℃~ Cultivate at 37°C for 24 hours to obtain test tube species;

[0030] 2) Liquid expansion culture: The formula of liquid medium is: every 1000ml of distilled water contains 3g of beef extract, 10g of peptone, pH7.2~7.4, inoculate the test tube species of Bacillus pumilus into 250ml of Erlenmeyer flask liquid medium, each bottle contains 100ml, Cultivate on a shaking table at 25°C-37°C for 24 hours, with a rotation speed of 150-250rpm.

[0031] 2. Bacillus pumilus ( Bacillus pumilus ) nematicide activity test

[0032] 1) Preparation of whole-tooth revived nematodes ( Panagrellus redivivus ) nematod...

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Abstract

The invention relates to a Bacillus pumilus and an application thereof on killing eelworms. The morphological characteristics of the Bacillus pumilus strain with the function of killing eelworms are as follows: the strain is short rhabditiform, the size of the thallus is 0.58*2.1 microns, and the strain has endogenous spores and is positive by Gram staining. A bacterial colony protrudes on a beef extract-peptone solid culture medium, the edge is smooth and fine, the bacterial colony is milk white and the size is 1.1-1.8 mm; the bacterial colony is smaller on a DSMZ88 improved solid culture medium, the bacterial colony is white, the surface of the bacterial colony is more dry, the edge is rough and irregular and the diameter is 1.5-2.0 mm. The Bacillus pumilus is prepared by a regular method. The Bacillus pumilus is intermediately cultured and the insect-killing effects on Panagrellus redivivus, Meloidogyne incognita and ovum of the Meloidogyne incognita are detected. The Bacillus pumilus provided by the invention has better killing effects on the P.redivivus, the Meloidogyne incognita and the ovum of the Meloidogyne incognita and can be developed as an eelworm anti-biological inoculant.

Description

technical field [0001] The present invention relates to the technical field of microbial pesticides, specifically a kind of bacillus pumilus ( Bacillus pumilus ) application in killing nematodes. Background technique [0002] Plant parasitic nematodes are a common plant disease worldwide. There are more than 70 known species of root-knot nematodes alone, which harm more than 3,000 plants and cause huge losses every year. Chemical nematicides have played an important role in nematode control since their discovery in the 1940s. With the advancement of science and technology, many chemical nematicides have been found to have side effects, causing environmental pollution and endangering human health. Many have been disabled or will be disabled one after another. In particular, plant parasitic nematodes occur in the roots of crops. Due to the particularity of the soil environment, it must be applied in large doses to ensure its control effect. However, large doses of chemical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P5/00C12N1/20A01K67/033C12R1/01
CPCY02A40/22
Inventor 徐洁常寿荣王绍坤罗华元陶南
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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