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Paecilomyces lilacinus space mutation mutant strain Sd-m-16 and microbial preparation and application thereof

A technology of Paecilomyces lilacinus and microbial preparations, applied in the field of Paecilomyces lilacinus

Active Publication Date: 2014-02-26
四川金珠生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing Paecilomyces lilacinus strains need to be improved in terms of growth rate, spore production and other growth performance, as well as pathogenicity to root-knot nematode eggs. Mutant strains with significantly improved performance or pathogenicity to root-knot nematode eggs, which will have important application value for the biological control of root-knot nematodes

Method used

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  • Paecilomyces lilacinus space mutation mutant strain Sd-m-16 and microbial preparation and application thereof
  • Paecilomyces lilacinus space mutation mutant strain Sd-m-16 and microbial preparation and application thereof
  • Paecilomyces lilacinus space mutation mutant strain Sd-m-16 and microbial preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of Paecilomyces lilacinus wettable powder

[0030] 1. Preparation of Sd-m-16 spore powder of Paecilomyces lilacinus (P.lilacinus) aerospace mutation mutant strain

[0031] (1), preparation of Paecilomyces lilacinus fermentation broth

[0032] 1. After activating the Paecilomyces lilacinus space mutation mutant strain Sd-m-16, carry out shake flask seed culture;

[0033] The composition of shake flask seed culture medium (by mass percentage):

[0034] Glucose 1.5-2%

[0035] Sodium nitrate 0.4-0.6%

[0036] Ammonium sulfate 0.2-0.3%

[0037] Soy flour 0.2-0.3%

[0038] Dipotassium hydrogen phosphate 0.1-0.2%

[0039] Magnesium sulfate 0.1-0.2%

[0040] water balance

[0041] Liquid fermentation conditions: put 200ml of seed medium into a 500ml triangular flask, inoculate Paecilomyces lilacinus spore suspension after autoclaving, the inoculation amount is 1.5-3%, place at 24-28°C, shaker 150-200r / min, cultivated for 18-36h.

[0042] 2. Sec...

experiment example 1

[0071] Experimental example 1 Screening of Paecilomyces lilacinus 20-7 space-flight mutagen and determination of mutagenic effect

[0072] 1 Experimental materials and methods

[0073] 1.1 Strains

[0074] For the test Paecilomyces lilacinus (P.lilacinus) 20-7, Shandong (Sd) strains. After spaceflight mutagenesis and returning to the ground, it is stored in the laboratory at 4°C for future use.

[0075] 1.2 Aerospace carrying

[0076] Paecilomyces lilacinus 20-7 and Shandong (Sd) strains were divided into two parts respectively, one part was left on the ground for routine storage as a control (CK); the other part was carried on "Shenzhou" 8 spaceship, which lasted 6d20h. After returning to the ground, take out the materials in time and store them at low temperature.

[0077] 1.3 Mutant Screening

[0078] The Paecilomyces lilacinus strain and the wild-type strain (CK) treated by aerospace mutagenesis were respectively made into spore suspensions and diluted to 10 3 times...

experiment example 2

[0122]Experimental example 2 Determination of the pathogenicity of Paecilomyces lilacinus 20-7 spaceflight mutagen to root-knot nematode eggs

[0123] 1. Experimental method

[0124] Prepare 10 with sterile water 3 / mL root-knot nematode egg suspension, add 0.5mL of the egg suspension, 50μL spore liquid, and 4.5mL sterile water to a 6cm small Petri dish. A small petri dish without spore solution was used as a control. Each mutant strain was repeated 3 times, that is, 3 dishes for each. Incubate at 25°C for 4 days. Microscopic examination, 50 eggs were randomly counted in each dish, the number of parasitized eggs was counted, and the egg parasitism rate of each bacterial strain was calculated. Egg parasitism rate (%) = (average number of parasitized eggs in 3 repeated tests of this strain / 50) × 100%.

[0125] 2. Experimental results

[0126] It has been observed that root-knot nematode eggs can be infected 2 days after inoculation, and most of the parasitized eggs are egg...

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Abstract

The invention discloses a paecilomyces lilacinus space mutation mutant strain Sd-m-16 and a microbial preparation and an application thereof. A paecilomyces lilacinus strain 20-7 and a Shandong strain are carried on the Shenzhou 8 spacecraft for space mutation, and a non-carried original strain is used as a contrast to compare the variability of the space-carried strain on aspects of configuration, pigment change, growth speed, sporulation quantity, pathogenicity and the like to screen a mutant strain with improved growth performance and greatly improved pathogenicity on root-knot nematode eggs. The screening on the growth speed, sporulation quantity and pathogenicity of the space-carried strain indicates that wide positive mutation has been generated on such aspects of the space mutant strain Sd-m-16 of the Shandong strain as growth property, pathogenicity to root-knot nematode eggs and the like; particularly, the pathogenicity of the mutant strain is greatly improved in comparison with that of the original strain. The invention further provides a microbial preparation containing the space mutant strain and an application of the space mutant strain in biological prevention and cure of root-knot nematodes.

Description

technical field [0001] The present invention relates to Paecilomyces lilacinus (Paecilomyces lilacinus) aerospace mutation mutant strain, especially relates to Paecilomyces lilacinus Shandong strain aerospace mutation mutant strain Sd-m-16 and microbial preparation thereof, the present invention further relates to this mutant strain in The application in biological control of root-knot nematode belongs to the field of screening and application of Paecilomyces lilacinus mutant strains. Background technique [0002] Space Mutation Breeding, also known as Space Mutation Breeding, is a new breeding technology that combines aerospace technology with traditional physical, chemical mutagenesis and molecular technology. The effects of special space environmental conditions (microgravity, strong cosmic rays, high vacuum, microgravity, etc.) cause chromosomal aberrations in organisms, which in turn lead to genetic variation in organisms. After ground selection tests, they can be bred ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/79
Inventor 王曦茁汪来发王源郭志斌马建伟
Owner 四川金珠生态农业科技有限公司
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