Sporolactobacillaceae and produced preparation of living fungus thereof

A technology of Bacillus sp. and inoculum, which is applied in the directions of bacteria, animal feed, animal feed, etc., can solve the problems of low production and use costs, and achieve the effects of low production cost, improved production efficiency, and reduced piglet diarrhea rate.

Inactive Publication Date: 2005-10-26
NANJING AGRICULTURAL UNIVERSITY
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  • Sporolactobacillaceae and produced preparation of living fungus thereof
  • Sporolactobacillaceae and produced preparation of living fungus thereof
  • Sporolactobacillaceae and produced preparation of living fungus thereof

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Embodiment Construction

[0022] 1 Isolation and identification of strains

[0023] Take the piglet intestinal chyme and intestinal wall by aseptic method, dissolve them in sterile saline, and mix the diluted solution with a vortex mixer, 1000r.min. -1 After centrifugation, the supernatant was serially diluted, inoculated on solid medium, and cultured at 37°C for several days. Pick a few colonies from it. The results of strain optimization show that the growth rate of S1 bacteria is extremely fast, and it can reach a plateau 10 hours after inoculation. See image 3 ; S1 bacteria have the best resistance to low pH value, high temperature, high bile salt concentration and inhibition of Escherichia coli and Staphylococcus aureus, the bacteria identified as Sporolactobacillus sp. The main biological characteristic is G + , slender rod-shaped, segmented, oval spores, spores are obvious after malachite green staining, facultative anaerobic growth. The bacteria can be produced by the general fermentation e...

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Abstract

The present invention provides a kind of bacterium and its procreation of living bacterium reagent, which can reduce the rate of piglet diarrhea. The used of bacterium is gelanshi pigmentation feedback electropositive bacterium S1, according to appraisal, it is Sporolactobacillus sp..Its main biological character is G+, the shape of bacterium is slim, divided burl, elliptical sporangium, after malachite pigmentation pigmentation is obvious, concurrently, exclude oxygen development, endure lower pH(pH2.5) and higher saline chroma(0.2%), not sensetive to pepsin, endure high temperature, it can restrain coliform and golden ball bacterium well. It indicated from the track of molecule biological technology, this bacterium can propagated in alvine of piglet. The flow chart is bevel bacterium--- rocky bottle seed liquid--- ferment jar---freez dryness--- production. The animal test indicates, this bacterium can be used directly and widely in reduce alvine of piglet, enhance the growth rate of piglet, reduce the use of antibiotics in piglet feedstuff.

Description

1. Technical field [0001] The Bacillus sp. Lactobacillus S1 and the microbial preparation thereof of the present invention belong to the high-tech field of biology, reduce the diarrhea rate of piglets and improve the growth rate of piglets by adjusting the microecological method of animal intestinal tract, and are suitable for modern agriculture Production of green and pollution-free animal products in production. 2. Technical background [0002] Since the US Food and Drug Administration (FDA) first approved antibiotics as feed additives in the 1950s, countries around the world have successively conducted feeding trials of antibiotics and used them in production. Undoubtedly, antibiotics used as growth-promoting feed additives have played an active role in preventing animal diseases and promoting animal growth. But over time and scientific advances, it has been recognized that the use of antimicrobials such as feed antibiotics in rearing can have serious negative effects. ...

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Application Information

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IPC IPC(8): A23K10/18A23K50/30A23K50/60C12N1/20
Inventor 朱伟云姚文毛胜勇
Owner NANJING AGRICULTURAL UNIVERSITY
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