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Paracoccus MXX-04 for bromoxynil degradation and application thereof

A technology of MXX-04 and bromoxynil, which is applied in the field of environmental microorganisms to achieve good removal effects, ecological environment protection, and low production costs

Active Publication Date: 2013-08-14
JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biodegradation is the main way to remove bromoxynil in nature. At present, there is no research report on bromoxynil-degrading bacteria in China. Therefore, microbial strains that efficiently degrade bromoxynil are isolated from bromoxynil-polluted environments. The role of remediation of bromoxynil pollution in the environment has great theoretical significance and potential application prospects

Method used

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  • Paracoccus MXX-04 for bromoxynil degradation and application thereof
  • Paracoccus MXX-04 for bromoxynil degradation and application thereof
  • Paracoccus MXX-04 for bromoxynil degradation and application thereof

Examples

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Effect test

Embodiment 1

[0024] Example 1 Isolation and identification of bromoxynil-degrading bacteria MXX-04

[0025] Take the bromoxynil-enriched bacterial solution and perform gradient dilution. The dilution is spread on the inorganic salt solid medium containing 50mg / L bromoxynil, and the medium formula is: every liter contains 1.5 g K 2 HPO 4 , 0.5 g KH 2 PO 4 , 0.2 g MgSO 4 ×7H 2 O, 1.0 g NaCl, 18 g agar, pH = 7.0, culture at 30°C for several days. A single colony was picked from it, and its degradation effect was verified by HPLC, and a strain with higher degradation efficiency was stored in a -20°C refrigerator with 30% glycerol. See the picture of its colony figure 1 , identified as Paracoccus (Paracoccus sp.); named: MXX-04. The main biological characteristic is G - , short rod-shaped, no spores, no motility, positive for oxidase and catalase. Its crystal violet staining photo is as figure 2 As shown, it can grow and mineralize bromoxynil as the only nitrogen source. In the sh...

Embodiment 2

[0026] Embodiment 2 Bromoxynil-degrading bacteria MXX-04 produces the method for bacterial agent

[0027] The original seed of the bromoxynil-degrading bacteria MXX-04 of the present invention was activated on a petri dish and in an incubator, at 30° C. for 30 h, and its degradation performance was measured. After the colony was mature, it was inoculated on the inclined surface of a test tube for later use. The test tube seed was inoculated in a 1000ml shake flask containing 200ml LB medium, LB medium formula (g / L): yeast extract 5.00, peptone 10.00, NaCl 10.00, pH 7.0, constant temperature shaking culture to the logarithmic phase, ready to inoculate the seed tank . The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH4) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2. After the feeding is completed, sterilize under high pressure at 121°C, cool to 33°C, inoculate the abov...

Embodiment 3

[0028] Embodiment 3 Bromoxynil-degrading bacteria MXX-04 produces the method for bacterial agent

[0029]Activate the original seed of the bromoxynil-degrading bacteria MXX-04 of the present invention on a petri dish and in an incubator, and measure its degradation performance at 30° C. for 30 hours. After the colonies are mature, they are inoculated on the inclined surface of a test tube for later use. The test tube seed was inoculated into a 1000ml shake flask containing 200ml LB medium, LB medium formula (g / L): yeast extract 5.00, peptone 10.00, NaCl 10.00, pH 7.0, constant temperature shaking culture to logarithmic phase, ready to inoculate the seed tank . The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH4)2SO4 1%, K2HPO4 0.2%, MgSO4 0.05%, NaCl 0.01%, CaCO3 0.3%, yeast extract 0.02%, pH value 7.5 . After the feeding is completed, sterilize under high pressure at 121°C, cool to 33°C, inoculate the above cultured shake...

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Abstract

The invention provides a degradation strain for eliminating residual bromoxynil, belonging to the field of biological high technology. The adopted bacterial strain is a gram staining reaction negative strain MXX-04 which is identified to be paracoccussp. The main biological properties are G-, short rod shape, no spores, no motility and positive oxidase and catalase. The strain provided by the invention can grow by taking bromoxynil as a unique nitrogen source and mineralize the bromoxynil. Under the condition of a shake flask in a laboratory, the strain can degrade 95.3% of 100mg / L bromoxynil within 24 hours, thus the problem that bromoxynil is hard to degrade biologically in the environment is solved.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a bromoxynil-degrading Paracoccus MXX-04 and an application thereof. Background technique [0002] The extensive use of herbicides has had a huge impact on the environment. First of all, the use of chemical herbicides not only effectively controls weeds, but also reduces the number of microorganisms in the soil and the activity of some enzymes in the soil to a certain extent, thereby affecting soil quality and fertilizer supply capacity, and the greater the amount of application, the greater the impact. Big. Second, herbicides can pollute groundwater, thereby affecting human health. [0003] Bromoxynil is widely used abroad as a contact herbicide for selective post-emergence stem and leaf treatment in the form of its octanoate, sodium salt, and potassium salt. It is mainly absorbed by the leaves, and rapidly causes tissue necrosis by inhibiting the various...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/01A62D101/04A62D101/26
Inventor 张永乐蔡双梅邱沪生顾乔梅
Owner JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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