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Automatic staining instrument with Gram staining two-step method

A Gram dyeing and dyeing machine technology, applied in the field of automatic dyeing machines, can solve problems such as complicated operation, and achieve the effect of strong continuity, friendly interface and high efficiency

Inactive Publication Date: 2011-10-19
叶元康
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The 4-step staining effect of Gram staining is very good, but the operation is more complicated. Modern laboratories are busy with work, and there are requirements for simplicity in various technical methods in order to complete more tasks in a limited time.

Method used

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  • Automatic staining instrument with Gram staining two-step method
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A two-step automatic staining instrument for gram staining, its structure is as follows figure 1 Shown. The dyeing machine is composed of the first dyeing tank 1, the second dyeing tank 2, the cleaning tank 3, the warm air drying tank 4, the manipulator 5, the track 6 and the display screen 7. The first dyeing tank 1, the second dyeing tank 2, the cleaning The pool 3 and the warm air drying pool 4 are arranged in sequence in the front of the dyeing machine. The dye in the first dyeing tank 1 is a 0.5wt% crystal violet aqueous solution, and the dye in the second dyeing tank 2 is an ethanol solution of basic fuchsin, iodine and potassium iodide, in which the concentration of basic fuchsin is 0.3wt%, The concentration of iodine is 0.1wt%, and the concentration of potassium iodide is 0.6wt%. The overflow drainage through the cleaning tank 3 will not cause the overflow of dye liquor and environmental pollution during dyeing. The temperature in the heating drying tank 4 is 50...

Embodiment 2

[0041] A two-step automatic dyeing instrument for gram dyeing. The dyeing instrument is composed of a first dyeing tank, a second dyeing tank, a cleaning tank, a warm air drying tank, a manipulator, a track, and a display screen. The pool, the cleaning pool and the warm air drying pool are arranged in sequence in the front of the dyeing machine. The dye in the first dyeing tank is a 1.0wt% crystal violet aqueous solution, and the dye in the second dyeing tank is an ethanol solution of basic fuchsin, iodine and potassium iodide. The concentration of basic fuchsin is 0.2wt%, and the concentration of iodine is 0.1wt%, the concentration of potassium iodide is 0.4wt%, the overflow drain through the cleaning tank, during dyeing, will not cause the overflow of the dye solution and environmental pollution, the temperature in the heating flow drying tank is 50℃, without flame fixation and natural drying. The manipulator is set on the upper part of the dyeing machine. The front end of th...

Embodiment 3

[0044] A two-step automatic dyeing instrument for gram dyeing. The dyeing instrument is composed of a first dyeing tank, a second dyeing tank, a cleaning tank, a warm air drying tank, a manipulator, a track, and a display screen. The pool, the cleaning pool and the warm air drying pool are arranged in sequence in the front of the dyeing machine. The dye in the first dyeing tank is a 0.5wt% crystal violet aqueous solution, and the dye in the second dyeing tank is an ethanol solution of basic fuchsin, iodine and potassium iodide. The concentration of basic fuchsin is 0.4wt% and the concentration of iodine is 0.2wt%, the concentration of potassium iodide is 0.6wt%. The overflow drainage through the cleaning tank will not cause the overflow of dye liquor and environmental pollution during dyeing. The temperature in the heating flow drying tank is 60°C, without flame fixation and natural drying. The manipulator is set on the upper part of the dyeing machine. 24 sheets to be dyed are...

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Abstract

The invention relates to an automatic staining instrument with a Gram staining two-step method. The staining instrument comprises two staining pools, a cleaning pool, a hot air flow drying pool, a manipulator, a rail and a display screen, wherein the first staining pool, the second staining pool, the cleaning pool and the hot air flow drying pool are sequentially arranged at the front part of the staining instrument, the manipulator is arranged at the upper part of the staining instrument, slices to be stained or quality control slices can be placed in the front end of the manipulator, the back end of the manipulator is connected with the rail, and the display screen is arranged at one side of the top of the staining instrument. Compared with the prior art, the instrument has the advantages that the protective technology and the operation flow are simple, special requirements to water quality are free, no harmful organisms are introduced and produced during the production and detection processes, more quick and easily-standardized experiment results can be provided, the result is easy to observe and judge, and the safety and reliability are provided.

Description

Technical field [0001] The invention relates to an automatic staining instrument, in particular to a two-step automatic staining instrument for gram staining. Background technique [0002] Gram staining is a widely used differential staining method in bacteriology. It was founded in 1884 by the Danish physician Gram. Bacteria are dyed with basic dye crystals first, and after mordanting with iodine solution, they are decolorized with alcohol. Under certain conditions, some bacteria will not be stripped off, and some can be stripped off. Therefore, bacteria can be divided into two categories The former is called Gram-positive bacteria (G+), and the latter is Gram-negative bacteria (G-). For the convenience of observation, a red dye such as basic safranin is used for counterstaining after decolorization. Positive bacteria are still purple, while negative bacteria are stained red. Bacillus with spores and most cocci, as well as all defensive bacteria and fungi show a positive Gram...

Claims

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Application Information

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IPC IPC(8): C12M1/34G01N1/31
Inventor 叶元康倪语星
Owner 叶元康
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