Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of tobacco cell extract

An extract and cell technology, applied in the field of engineering cell culture and extraction, can solve the problems of unsatisfied demand for tobacco raw materials, unstable raw material sources, fluctuation of extract quality, etc., and achieves a short processing cycle, reduced stimulation, and effective The effect of high content of ingredients

Active Publication Date: 2016-08-17
HUBEI CHINA TOBACCO IND +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 7) There are many breeding methods and many trial varieties
[0013] 2) A large amount of arable land needs to be sacrificed, and at the same time fertilization of tobacco leaves can easily cause soil compaction, causing serious imbalance of land nutrients;
[0014] 3) Due to the instability of raw material sources, the quality of the extract fluctuates;
[0015] 4) Due to the limited tobacco leaf resources, the demand for tobacco leaf raw materials cannot be met

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of tobacco cell extract
  • Preparation method of tobacco cell extract
  • Preparation method of tobacco cell extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation method of tobacco cell extract, comprises the following steps:

[0040] 1) Acquisition of callus tissue

[0041] Select the bare seeds of conventional flue-cured tobacco varieties, wash them twice with alcohol with a volume fraction of 75%, and then use HgCl with a mass fraction of 0.1%. 2 The solution was treated for 6 minutes, washed with sterile water for 5 times, and then the sterilized seeds were sowed on the sterilized MS solid medium without sucrose and hormones, and cultivated under the light condition of 25±1°C for 3 weeks, then selected Hypocotyls, shoot tips, etc. are used as explants, shredded and transferred to MS induction solid medium, and cultured for 4 weeks under light conditions at 25±1°C to obtain callus; the MS induction medium contains mass fraction 0.05% of naphthaleneacetic acid (NAA), 0.15% of 2,4-dichlorophenoxyacetic acid (2,4-D), 0.01-0.5% of 6-benzylpurine (6-BA ) and sucrose with a mass fraction of 0.1-1.0%.

[0042] 2) P...

Embodiment 2

[0052] The preparation method of tobacco cell extract, comprises the following steps:

[0053] 1) Acquisition of callus tissue

[0054] Select the bare seeds of conventional flue-cured tobacco varieties, wash them twice with alcohol with a volume fraction of 75%, and then use HgCl with a mass fraction of 0.1%. 2 The solution was treated for 10 minutes, washed with sterile water for 5 times, and then the sterilized seeds were sowed on the sterilized MS solid medium without sucrose and hormones, and cultivated under the light condition of 25±1°C for 3 weeks. As explants, hypocotyls and shoot tips were cut into pieces and transferred to MS induction solid medium, and cultured for 6 weeks under the light condition of 25±1°C to obtain callus tissue; among them, the mass fraction of MS induction medium contained 0.05% of naphthalene acetic acid, 0.15% of 2,4-dichlorophenoxyacetic acid, 0.01-0.5% of 6-benzylpurine and 0.1-1.0% of sucrose.

[0055] 2) Preparation of cells

[0056] ...

Embodiment 3

[0065] The preparation method of tobacco cell extract, comprises the following steps:

[0066] 1) Acquisition of callus tissue

[0067] Select the bare seeds of conventional flue-cured tobacco varieties, wash them twice with alcohol with a volume fraction of 75%, and then use HgCl with a mass fraction of 0.1%. 2 The solution was treated for 8 minutes, rinsed with sterile water for 3 times, and then the sterilized seeds were sowed on the sterilized MS solid medium without sucrose and hormones, and cultured for 5 weeks under the light condition of 25±1°C. As explants, hypocotyls, shoot tips, etc., cut into pieces and moved into MS induction solid medium, and cultured for 3 weeks under light conditions at 25±1°C to obtain callus; among them, MS induction medium contains mass fraction 0.3% naphthalene acetic acid, 0.25% 2,4-dichlorophenoxyacetic acid, 0.01-0.5% 6-benzylpurine and 0.1-1.0% sucrose.

[0068] 2) Preparation of cells

[0069] The callus obtained in step 1) was cont...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing tobacco cell extract. The tobacco cell extract is obtained through the five steps of acquisition of calluses, preparation of cells, preparation of protoplast and acquisition of cell clones, collection of clone cells and preparation of the tobacco cell extract. The method has the advantages that effective tobacco resources are not occupied, controllability is high, the processing period is short, investment is low, pollution is avoided, the content of effective ingredients of the prepared tobacco cell extract is high, the fragrant, sweet and faint scent and gentle and delicate smoke can be provided for cigarettes, the smoke concentration is increased, the fragrance amount is increased, the sweet taste is improved, and stimulation is reduced.

Description

technical field [0001] The invention relates to a method for cultivating and extracting engineered cells, in particular to a method for preparing tobacco cell extract. Background technique [0002] Plant tissue culture is plant aseptic culture technology, also known as in vitro culture, which is based on the theory that plant cells are omnipotent, using the isolated organs of plants (such as roots, stems, leaves, stem tips, flowers, fruits, etc.), Tissues (such as cambium, epidermis, cortex, medullary cells, endosperm, etc.) or cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts, under artificial conditions such as sterile and suitable artificial medium and temperature , can induce callus, adventitious buds, adventitious roots, and finally form a complete plant discipline. Plant tissue culture has the characteristics that the culture conditions can be controlled artificially, the growth cycle is short, the reproduction rate is high, and the manageme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A24B15/26C12N5/04
CPCA24B15/26C12N5/04
Inventor 喻世涛熊国玺苗丽坤王萍
Owner HUBEI CHINA TOBACCO IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com