Screening of phosphorus and potassium decomposing bacillus and application of phosphorus and potassium decomposing bacillus to improvement on greenhouse vegetable soil hardening
A technology of bacillus and soil compaction, applied in the application, soil conditioning materials, bacteria and other directions, can solve the problems of soil compaction, affecting the sustainable development of greenhouse vegetables, improper fertilization technology, etc.
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Embodiment 1
[0006] Embodiment one, strain screening
[0007] Bacillus JJMSH1 was isolated from the soil of vegetable greenhouses. In order to realize this invention, organic phosphorus medium was used for primary screening to enrich, and then inorganic phosphorus medium was used for rescreening, and then the strains with phosphorus-solubilizing function were spot-connected to silicate bacterial medium . Therefore, strains that may have the effect of dissolving phosphorus and potassium can be obtained at the same time.
[0008] Firstly, use organic phosphorus medium for enrichment, take 1g greenhouse vegetable rhizosphere soil sample in 100ml enrichment medium, enrich at 28°C and 200r / min for 2 days. Afterwards, dilute the gradient with sterile normal saline to 10-8, then spread the three gradients of -6, -7, and -8 on the inorganic phosphorus medium, cultivate at 30°C, observe the growth every day, observe the hydrolysis circle of the inorganic phosphorus medium, and Record the diameter...
Embodiment 2
[0015] Embodiment two, the determination of available phosphorus and available potassium
[0016] The present invention uses the molybdenum antimony anti-colorimetric method to measure the available phosphorus content, and the flame photometry to measure the available potassium content
[0017] Determination of available phosphorus by molybdenum antimony anti-colorimetric method
[0018] The principle of molybdenum antimony anticolorimetric method: 2,4-dinitrophenol is used as indicator, and soluble phosphate can react with molybdenum antimony antichromogenic agent to generate phosphomolybdenum blue. The colorimetric determination is carried out at a wavelength of 660nm, according to the standard The curve calculates the content of available phosphorus, and the concentration of available phosphorus indicates the level of microbial phosphorus-dissolving ability.
[0019] Commonly used reagents for molybdenum antimony anti-colorimetric method
[0020] 1. Molybdenum antimony an...
Embodiment 3
[0033] Embodiment three, bacterial strain JJMSH1 strain identification
[0034]The surface of the colony is rough, opaque, and the color is milky white and slightly yellowish, similar to the colony morphology of certain strains of Bacillus
[0035] Gram stain and spore stain
[0036] Gram staining: fix the smear, stain with crystal violet for 1 minute, rinse with tap water, be careful not to wash off the bacteria, mordant with iodine solution for about 1 minute, wash with water, absorb water with absorbent paper, 1 drop of 95% alcohol, 15~ After 20 seconds, wash with water, absorb the water, counterstain with safranin staining solution for 1 minute, rinse with tap water, and observe with an oil microscope.
[0037] The bacterial strain of the present invention is identified as a positive bacterium through Gram staining, and the bacterium is long rod-shaped, and similar uncolored spores are occasionally produced.
[0038] Spore staining
[0039] Smear, dry, and fix, add 3-5 ...
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