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Screening of phosphorus and potassium decomposing bacillus and application of phosphorus and potassium decomposing bacillus to improvement on greenhouse vegetable soil hardening

A technology of bacillus and soil compaction, applied in the application, soil conditioning materials, bacteria and other directions, can solve the problems of soil compaction, affecting the sustainable development of greenhouse vegetables, improper fertilization technology, etc.

Inactive Publication Date: 2017-07-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the accumulation of cultivation time and improper fertilization techniques, soil compaction is easy to occur, which seriously affects the sustainable development of greenhouse vegetables.

Method used

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  • Screening of phosphorus and potassium decomposing bacillus and application of phosphorus and potassium decomposing bacillus to improvement on greenhouse vegetable soil hardening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0006] Embodiment one, strain screening

[0007] Bacillus JJMSH1 was isolated from the soil of vegetable greenhouses. In order to realize this invention, organic phosphorus medium was used for primary screening to enrich, and then inorganic phosphorus medium was used for rescreening, and then the strains with phosphorus-solubilizing function were spot-connected to silicate bacterial medium . Therefore, strains that may have the effect of dissolving phosphorus and potassium can be obtained at the same time.

[0008] Firstly, use organic phosphorus medium for enrichment, take 1g greenhouse vegetable rhizosphere soil sample in 100ml enrichment medium, enrich at 28°C and 200r / min for 2 days. Afterwards, dilute the gradient with sterile normal saline to 10-8, then spread the three gradients of -6, -7, and -8 on the inorganic phosphorus medium, cultivate at 30°C, observe the growth every day, observe the hydrolysis circle of the inorganic phosphorus medium, and Record the diameter...

Embodiment 2

[0015] Embodiment two, the determination of available phosphorus and available potassium

[0016] The present invention uses the molybdenum antimony anti-colorimetric method to measure the available phosphorus content, and the flame photometry to measure the available potassium content

[0017] Determination of available phosphorus by molybdenum antimony anti-colorimetric method

[0018] The principle of molybdenum antimony anticolorimetric method: 2,4-dinitrophenol is used as indicator, and soluble phosphate can react with molybdenum antimony antichromogenic agent to generate phosphomolybdenum blue. The colorimetric determination is carried out at a wavelength of 660nm, according to the standard The curve calculates the content of available phosphorus, and the concentration of available phosphorus indicates the level of microbial phosphorus-dissolving ability.

[0019] Commonly used reagents for molybdenum antimony anti-colorimetric method

[0020] 1. Molybdenum antimony an...

Embodiment 3

[0033] Embodiment three, bacterial strain JJMSH1 strain identification

[0034]The surface of the colony is rough, opaque, and the color is milky white and slightly yellowish, similar to the colony morphology of certain strains of Bacillus

[0035] Gram stain and spore stain

[0036] Gram staining: fix the smear, stain with crystal violet for 1 minute, rinse with tap water, be careful not to wash off the bacteria, mordant with iodine solution for about 1 minute, wash with water, absorb water with absorbent paper, 1 drop of 95% alcohol, 15~ After 20 seconds, wash with water, absorb the water, counterstain with safranin staining solution for 1 minute, rinse with tap water, and observe with an oil microscope.

[0037] The bacterial strain of the present invention is identified as a positive bacterium through Gram staining, and the bacterium is long rod-shaped, and similar uncolored spores are occasionally produced.

[0038] Spore staining

[0039] Smear, dry, and fix, add 3-5 ...

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Abstract

The invention relates to screening of phosphorus and potassium decomposing bacillus JJMSH1 and application of the phosphorus and potassium decomposing bacillus to improvement on greenhouse vegetable soil hardening. The strain has a good phosphorus and potassium decomposing function. The strain is screened from rhizosphere soil of a plant in a vegetable greenhouse; the soil is enriched through an organic phosphorus culture medium and then subjected to screening through an inorganic phosphorus culture medium and a silicate culture medium successively, and a molybdenum and antimony resistant colorimetric method and a flame photometry are used for detecting the contents of effective phosphorus and rapidly available potassium in sequence, thus finally selecting the JJMSH1 as an ideal strain. The JJMSH1 is authenticated by gram staining and spore staining to be gram-positive bacillus. When the strain disclosed by the invention is used for treating hardened soil in the greenhouse, the contents of the effective phosphorus and the rapidly available potassium in the soil are relatively obviously increased, and the OD600 reaches 1.5; after being diluted 100 times, a microbial inoculum is applied to the soil, so that the content of the effective phosphorus is increased by 35.7 percent, and the content of the rapidly available potassium is increased by 26.1 percent.

Description

technical field [0001] The invention is designed in the fields of agriculture and microorganisms, especially the strain has the effect of dissolving phosphorus and potassium, and can improve the phenomenon of soil compaction in greenhouses to a certain extent. Background technique [0002] Both phosphorus and potassium are essential nutrients for plant growth. Phosphorus is widely involved in plant photosynthesis and biochemical reactions in the body, and potassium is closely related to plant metabolism and growth. Phosphorus in the soil mainly exists in the form of organic phosphorus and inorganic phosphorus. Most of the organic phosphorus exists in the form of phytic acid, nucleic acid, and phospholipids, which cannot be absorbed directly. It must be transformed into the form of inorganic phosphorus under the action of microorganisms to be absorbed. More than 95% of the phosphorus in the soil cannot be directly absorbed and utilized by plants. The soluble potassium conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/20C09K17/14C12R1/07C09K101/00
CPCC12N1/02C09K17/14C09K2101/00C12N1/20
Inventor 韩业君丁建军彭小伟
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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