41 results about "Bacillidium" patented technology

The invention provides a biological organic fertilizer prepared by aerobic fermentation of a biogas residue and a preparation method thereof. The preparation method comprises the steps: mixing the biogas residue and a swelling agent to form a fermentation raw material, inoculating a compound microbial agent in the fermentation raw material, uniformly mixing for fermenting, and adding urea, diammonium phosphate, a potassium chloride inorganic salt, brown nitrogen-fixing bacteria, mycorrhiza fungi, silicate bacteria, photosynthetic bacteria acetic bacteria, bifidobacterium, and saccharomycetes after the fermentation is completed to prepare the biological organic fertilizer, wherein the compound microbial agent comprises strains of bacillus, pseudomonas, staphylococcus, streptomyces, penicillium, aspergillus and trichoderma. The compound microbial agent prepared by using the biological organic fertilizer has a pertinence to aerobic fermentation of the biogas residue, and is capable of effectively increasing the fermentation rate of the biogas residue, shortening the fermentation time and realizing high-additional value production, innocent treatment and recycling of the biogas residue; and the problem of resources and environment of villages and small towns is solved, and the rapid development of construction of new countryside and towns and cities can be promoted.

Compositions and methods are provided for benefiting plant growth. The compositions contain isolated bacterial or fungal strains having properties beneficial to plant growth and development that can provide beneficial growth effects when delivered in a liquid fertilizer in combination with a soil insecticide to plants, seeds, or the soil or other growth medium surrounding the plant or seed. The beneficial growth effects include one or a combination of improved seedling vigor, improved root development, improved plant health, increased plant mass, increased yield, improved appearance, improvedresistance to osmotic stress, improved resistance to abiotic stresses, or improved resistance to plant pathogens. The isolated bacterial strains include those of the Bacillus species including speciessuch as Bacillus pumilus, Bacillus licheniformis, and Bacillus subtilis.

The invention discloses a high-temperature-resisting lactic acid strain HT1 and a method for modulating silage, belonging to the technical field of microorganism application and silage modulation and process. The strain is HT1, which is identified to be lactobacillusrhamosussp, the collection number of the strain is CGMCC4483, the Genbank accession number of 16SrDNA of the strain is JF414107, and the strain has the main biological characteristics of Gram positive bacilli, homofermentative glucose, acidproof property, quick growth speed, wide breeding temperature range and special high temperature resistance.

The invention relates to screening of phosphorus and potassium decomposing bacillus JJMSH1 and application of the phosphorus and potassium decomposing bacillus to improvement on greenhouse vegetable soil hardening. The strain has a good phosphorus and potassium decomposing function. The strain is screened from rhizosphere soil of a plant in a vegetable greenhouse; the soil is enriched through an organic phosphorus culture medium and then subjected to screening through an inorganic phosphorus culture medium and a silicate culture medium successively, and a molybdenum and antimony resistant colorimetric method and a flame photometry are used for detecting the contents of effective phosphorus and rapidly available potassium in sequence, thus finally selecting the JJMSH1 as an ideal strain. The JJMSH1 is authenticated by gram staining and spore staining to be gram-positive bacillus. When the strain disclosed by the invention is used for treating hardened soil in the greenhouse, the contents of the effective phosphorus and the rapidly available potassium in the soil are relatively obviously increased, and the OD600 reaches 1.5; after being diluted 100 times, a microbial inoculum is applied to the soil, so that the content of the effective phosphorus is increased by 35.7 percent, and the content of the rapidly available potassium is increased by 26.1 percent.

A xyloglucanase having a relative xyloglucanase activity of atleast 50% at pH7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from e.g. Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from e.g. B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

The invention provides a biological emulsifier, which is prepared from the Bacillus licheniformis XS2-450 strain with the preservation number of CGMCC No.9430. The biological emulsifier obtained by the invention is used for treating oily sludge contaminated with high viscosity and heavy hydrocarbons. The emulsifying activity of the emulsifier obtained by the invention can reach 83.2%, and the emulsifying stability to liquid paraffin still maintains 72.8% after 30 days. At the same time, the biological emulsifier has a good deoiling effect when used in the treatment of high-viscosity and heavy hydrocarbon-contaminated sludge, which can reduce the oil content in the sludge from 26.2% to the current 1.5%, and the oil content is reduced by 94%. Above, compared with physical and chemical methods, the cost can be reduced by 30%, which shows great economic benefits and potential for large-scale application while saving costs and protecting the environment.

The present invention relates to the cloning and application of a novel glutamic acid decarboxylase gene. The glutamic acid decarboxylase obtained from Bacillus is induced and expressed in Escherichia coli and its properties are studied. It is found that the enzyme has a pH of 4.0 It has higher activity at ~pH6.0, the optimum temperature is between 45℃~60℃, Vmax is 150~200 U / mg, and Km is 7~9 mmol / L. The recombinant Escherichia coli containing the enzyme was transformed into whole cells, and 500 g / L L-glutamic acid was added by fed-batch feeding, at 37°C, in disodium hydrogen phosphate-sodium dihydrogen phosphate buffer, pH 7.0 After 12 h of conversion, 347.8 g / L of γ-aminobutyric acid was finally produced, and the molar conversion rate was 99.4%. Compared with the existing glutamic acid decarboxylase, the novel glutamic acid decarboxylase has higher catalytic efficiency and wider pH range, and is more suitable for industrial application.

The invention discloses a strain TX-4 used for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode and a preparation thereof, belonging to the technical field of preventing and treating diseases of crops and being specially used for biological prevention of the crops. The prevention result of field demonstration shows that the strain has the preventing effect of more than90 percent to the aphelenchoides besseyi disease and has preventing effect of more than 70 percent to the vegetable root knot nematode disease. The strain TX-4 for preventing diseases of aphelenchoides besseyi or vegetable root knot nematode is bacillus with the Latin systematic name of Paenibacillus spp., the strain has been preserved in China Center for Type Culture Collection on March 19, 2009and the preserving number is CCTCC No: M209038.

The invention discloses bio-fertilizer for efficient degradation of atrazine and a preparation method thereof. Wheat straws, plant ash, saw dust and self-made zymophyte fertilizer beneficial for strain survival and growth are compounded and the compound, Exiguobacterium strain BTAH1 and Shinella granuli HBZA0511 are mixed and are fermented to form the bio-fertilizer. The self-made zymophyte fertilizer is prepared from nutrition bases such as cake, wheat bran, corn straws and fish bone powder, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and lactobacillus rhamnosus through fermentation. The bio-fertilizer is sowed in the field so that soil atrazine herbicide residual quantity is fast reduced by 95% or more, is conducive to soil bioremediation, is suitable for green agricultural product production and improves soil organic content.

A stabilizer for heavy metals in the sediment in an aquaculture pond. The stabilizer comprises a microbial carrier and a composite microbial agent loaded on the microbial carrier, the microbial carrier being a mixture of Mailuoning herb residue biomass carbon powder, tea residue powder, Alternanthera philoxeroides powder, and a binder; a composite active bacterial liquid is obtained by mixing a sediment sample and deionized water so as to obtain a supernatant diluent, carrying out culturing so as to obtain a seed solution of Bacillus and yeast, adding an EM active bacterial liquid, and carrying out culturing under the condition of aerobic fermentation. The preparation method comprises: (1) converting Mailuoning herb residues into biomass carbon at a high temperature, and then mixing the biomass carbon with raw materials so as to obtain a microbial carrier; and (2) loading a composite microbial agent onto the microbial carrier. The sediment stacked with the heavy metal stabilizer and then fermented can be used as a base fertilizer and applied to soil, so that the fertility of the soil is improved; the effectiveness of heavy metals in the soil is reduced; the amount of an inorganic fertilizer used is decreased; the yield and quality of crops are improved. Also disclosed are a method for preparing a stabilizer for heavy metals in the sediment in an aquaculture pond, and an application of the stabilizer.

The invention discloses a method for spraying black locust on exposed rock walls of shale for efficient and rapid regreening and improving soil pH, and belongs to the technical fields of ecological restoration and soil improvement. The guest soil spraying method is used to spray mixed microorganisms, organic fertilizers, and soil on the exposed shale rock wall, and the greening plant is black locust, so as to achieve efficient and rapid regreening and improve soil pH. The mixed microorganisms are composed of Koukia X-22, Microbacterium X-26, Bacillus X-28, and Microbacterium X-18, and the mixed microorganisms are added to organic fertilizer and soil as fermentation broth. The weight ratio of the mixed microorganisms, organic fertilizer and soil is 1:1:8. This method realizes the synergy between microbial flora and leguminous nitrogen-fixing bacteria, promotes the rapid growth of Robinia pseudoacacia on the exposed shale rock wall, can significantly increase the organic matter content, available phosphorus content, and pH value of Robinia pseudoacacia soil, improve soil fertility, and reduce the application of chemical fertilizers. It can provide a low-cost, high-efficiency new method for efficient and rapid regreening of shale exposed rock walls.

The present invention relates to isolated polypeptides and isolated nucleic acid sequences encoding the polypeptides, as well as methods for producing and using the polypeptides in animal feed. An example of a polypeptide of the invention is the so-called L12 protein from Bacillus lichen form is ATCC 14580 which improves the Feed Conversion Ratio (FCR) and / or acts as a gut microflora modulator. The invention furthermore relates to the probiotic use of strains of Bacillus which produce proteins related to L12.

A xyloglucanase having a relative xyloglucanase activity of atleast 50% at pH7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from e.g. Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from e.g. B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

The present invention relates to clostridium 'daqing' clostridium and its uses. The present invention solves the problem it is difficult to remove or degrade polypropylene of oil field sewage. The clostridium 'daqing' clostridium -WL is preserved at CGMCC on January 10, 2007, preserving number being CGMCC No. 1911. The 'daqing' clostridium is Gram-positive bacilli, with endo-spore, facultative anaerobic, grows under pH 5.0-11.0,10-65 degree. The strain is variable in length, breadth being 0.4um-0.8um, length being 1.4um-4um, free of flagellum. The inventive 'daqing' clostridium -WL is a new strain, and can use polypropylene as unique carbon source which can be used in polypropylene biodegradation. 10ml 'daqing' clostridium -WL of concentration 6x10<6> per ml is added in oil field sewage with polypropylene concentration being 500mg / L and the polypropylene degradation rate is 35%-76% after 20 days.

The invention relates to a bacillus velezensis XG2 strain for producing phthalide components and application thereof, the strain is obtained by separating, purifying and culturing from rhizosphere soil of angelica sinensis, and is identified as bacillus through microorganism 16S rDNA sequencing. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2021766. A bacillus velezensis strain which is stable in character, simple in culture medium and good in growth is provided for large-scale fermentation preparation of phthalide components, a practical, simple and convenient fermentation culture method and a separation and extraction process thereof are established, and components obtained after fermentation liquor is extracted and concentrated can serve as a new source of active components of angelica sinensis. The bacillus velezensis XG2 can promote the germination of angelica sinensis seeds and improve the plant height and fresh weight of angelica sinensis seedlings.