29 results about "Bacillidium" patented technology

The invention discloses preparation of a microbial compound element-based fertilizer and application of the microbial compound element-based fertilizer in saline-alkali soil treatment, and relates to the technical field of saline-alkali soil improvement. The preparation method comprises the following steps: inoculating lactic acid bacteria, rhodopseudomonas, phosphate-solubilizing bacillus, nitrogen-fixing bacillus, halophilic bacillus and halomonas into a composite culture medium in proportion, and carrying out stepped sequential fermentation culture under the conditions that the pH value is 7.5 and the temperature is 28 DEG C until the bacterial count is greater than or equal to 1010 CFU / mL. According to the preparation of the fertilizer based on the microbial compound elements and the application of saline-alkali soil treatment, a synergistic system of the compound functional flora, the chelated mineral substances and the intelligent organic carrier is constructed, so that multi-dimensional improvement of saline-alkali soil is realized, the limitation of a traditional single improvement mode is broken through, the salinity and pH value of the soil are effectively reduced, and the soil quality is improved. The soil fertility and biological activity are remarkably improved, the salt stress resistance of crops is enhanced, the effective period is prolonged, and the preparation cost is low.

The application provides a method for treating and recycling iron-containing waste sulfuric acid in titanium dioxide production, which is based on screening of a strain capable of treating iron-containing waste water in a low pH environment, and establishes a method for treating iron-containing waste sulfuric acid, which can effectively realize resource recycling. The waste water treatment bacteria used are Bacillus QD-QJ-062 strain, and the preservation number is CGMCC No.33725. The QD-QJ-062 strain of Bacillus screened by the application can effectively treat the iron-containing waste water in a low pH environment, promote the generation of ferrous oxalate precipitation, and generate ferrous oxalate by-products. The generated ferrous oxalate can be converted into ferrous sulfate by sulfuric acid, and oxalic acid can be recycled, thereby reducing the operation cost. The sulfuric acid solution after iron removal can be neutralized by limestone to generate high-purity gypsum, thereby realizing resource recycling and treatment.

The invention discloses a method for preparing ganoderan rich in a mannogalactan structure from a Chryseobacterium bacterium GS-7 and Ganoderma lucidum YJ0724 and application of the ganoderan rich in the mannogalactan structure, and relates to the technical field of microbial fermention.The method comprises the following steps that a Chryseobacterium bacterium GS-7 seed solution is prepared, and a Ganoderma lucidum YJ0724 seed solution is prepared; the method comprises the following steps: a, simultaneously inoculating a seed solution of a Chryseobacterium bacterium GS-7 and a seed solution of Ganoderma lucidum YJ0724 into a fermentation medium for co-culture to obtain a fermentation solution containing Ganoderma lucidum polysaccharide rich in a mannan galactan structure; or b, filtering and degerming the fermentation liquor of the Chryseobacterium bacterium GS-7, and inoculating the fermentation liquor and the ganoderma lucidum YJ0724 seed solution into the culture medium for co-culture to obtain the fermentation liquor containing the ganoderma lucidum polysaccharide rich in the mannogalactan structure. The obtained ganoderma lucidum polysaccharide rich in the mannogalactan structure can improve the immunomodulatory activity.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The application provides a high-salt and high-nitrate nitrogen wastewater denitrification method, which comprises the following steps: S1, adjusting the pH of the high-salt and high-nitrate nitrogen wastewater to the range of 5.5-7.5, and adopting a primary full-mixed denitrification tank for denitrification, wherein a composite microbial inoculum is put into the primary full-mixed denitrification tank at one time, and the composite microbial inoculum comprises Marinobacter, Azoarcus, Paracoccus, Bacillus and Pseudomonas; S2, adopting a secondary full-mixed denitrification tank for denitrification of the effluent of step S1; during the operation of the secondary full-mixed denitrification tank, a mud-water mixture is backflowed to the primary full-mixed denitrification tank; S3, the effluent of step S2 is introduced into a degassing tank for degassing; S4, the effluent of step S3 is precipitated in a precipitation tank, and then the effluent is discharged. The composite microbial inoculum is used for treating the high-salt and high-nitrate nitrogen wastewater, and the problems of large treatment facility area and high cost caused by concentrated evaporation or dilution of wastewater and then biochemical treatment in traditional treatment are avoided.

The bacterial strain Arasana bacillus (CECT 30655) belongs to Gram-positive bacterial flora and bacillus subtilis, and is a stimulant capable of stimulating adaptive metabolism of plants to resist water stress, an improving agent for plant yield and nutrition and an improving agent for polyphenol content. The strain is separated from rhizosphere soil of pinus coast, is cultured on a nutrient agar culture medium (PCA), and is identified as a new strain from the aspects of morphology, biochemistry and genetics for identification through whole genome sequencing. Under a water stress condition, the strain can enhance CO2 fixation and transpiration, optimize photosynthesis energy capture and reduce oxidative stress, so that the strain can be used for increasing yield; as a biological fertilizer, nutrient absorption can be promoted no matter whether water stress exists or not; and the content of antioxidant polyphenols in plant species with medicinal or edible values can also be improved, so that the fruit quality is improved.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The invention relates to the field of soil pollution remediation, and discloses a micro-ecological reconstruction and remediation method for micro-plastic polluted soil, which comprises the following steps: firstly, screening and enriching micro-plastic degradation functional florae which have the advantages of pseudomonas and bacillus, and the bacillus can secrete lipase and protease; the preparation method comprises the following steps: mixing biomass charcoal prepared by pyrolysis of corn straws with a functional bacterium suspension for adsorption, and carrying out sodium alginate-calcium chloride crosslinking to prepare an immobilized composite repairing agent; applying a repairing agent accounting for 1-5% of the mass of the soil, uniformly mixing the repairing agent with the soil, regulating the soil humidity to 20-30% and the carbon-nitrogen ratio to (15-25): 1, and maintaining the environment temperature of 15-35 DEG C; microplastics are removed through the synergistic effect of biomass charcoal adsorption and functional flora enzymatic degradation, beneficial microorganisms are enriched synchronously, and functional gene expression is activated; the problems that in the prior art, functional flora planting is difficult, the degradation efficiency is low, micro-ecological restoration is deficient, and the environmental adaptability is poor are solved.

A surface treatment composition that includes from about 1×102 to about 1×109 CFU / g of the composition of Bacillus spores and from about 0.01% to about 5% by weight of the composition of a cationic polyglucan.

The invention discloses an efficient carbon sequestration bacillus cereus from an oil-containing solid waste residue source and application of the efficient carbon sequestration bacillus cereus. The bacillus cereus is any one of bacillus cereus S13 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2024515 and bacillus cereus S29 with the preservation number of CCT CC NO: M 2024516. The bacillus cereus strain is obtained by taking bacillus cereus S10 separated from oil-containing solid waste residues of an oil field as an original strain through ARTP mutagenesis and screening, has excellent characteristics of reducing pollution and sequestering carbon, is relatively strong in stress resistance, can be used for deeply recycling the oil-containing solid waste residues, can be used for improving the carbon sequestration capacity of petroleum-polluted soil and can be used for preparing the oil-containing solid waste residues. The method has a wide application prospect in the fields of petroleum-polluted soil remediation and the like, and has great significance in pollution reduction and convergence increase in petroleum and petrochemical industries under the background of supporting double carbon.

The invention relates to the technical field of water-preserved mining of coal mines, and discloses a system and method for repairing overlying strata water flowing fractures through microbial mineralization, and the system comprises a mineralization repairing agent preparation unit which is used for preparing a mineralization repairing agent based on a microbial bacterial solution, a mineralization precursor solution, a carbon source solution, a nutrient solution and / or a water chemical adjusting solution, the microorganisms adopt bacillus microorganisms with urea hydrolysis or carbonate mineralization capability; and the fracture injection unit is used for injecting the mineralization repairing agent into the overlying strata water diversion fracture zone between the mining coal seam and the shallow underground water. A biological-mineral composite flexible water-blocking structure can be formed in a repairing area, has interface adhesiveness and certain flexibility, can cooperatively deform along with slow sinking of overlying strata and the earth surface and stress adjustment, reduces secondary cracking and seepage channel reactivation, weakens the water guiding capacity among an underlying goaf, a working face and an overlying aquifer, and meanwhile, improves the water-blocking performance of the working face and the overlying aquifer. The water loss of shallow groundwater is reduced, and reliable technical support is provided for water-preserved mining under the aquifer.

The invention discloses a bacillus cereus strain and application thereof, a phylogenetic tree is constructed through whole genome sequencing according to housekeeping genes, the phylogenetic tree is determined to belong to bacillus cereus (Bacillus cereus), and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.34800. The strain B2 obtained through separation can secrete indoleacetic acid (IAA), IAA serves as auxin and is beneficial for stimulating plant root development and promoting root growth, so that the water and nutrient absorption capacity of plants is enhanced, the adaptability of the plants in a stress environment is improved, meanwhile, the strain B2 can regulate and control sodium and potassium ion transport in pepper plants, and the yield of the pepper plants is increased. Specifically, the expression level of the key ion transporter gene in the pepper is up-regulated. The bacillus cereus strain B2 is an efficient microbial resource and can be used for developing a microbial inoculant or a biological fertilizer so as to promote growth of crops such as capsicum in salinized soil, improve the salt resistance of the crops and guarantee and improve the sustainable production capacity of capsicum in the salinized soil.

The invention discloses a uniform and rapid pressure relief and permeability increase method for a deep coal seam, which aims at the complex geological conditions of high ground stress and high gas pressure of the deep coal seam and realizes efficient pressure relief and permeability increase through the synergistic effect of hydraulic slotting, hydraulic fracturing and biological degradation and elimination technologies. Firstly, through geological analysis and intelligent optimization design of drill hole arrangement, uniform coverage is ensured; then, high-pressure water is used for slotting to form a guide slot, and slag removal is facilitated; then, section-by-section fracturing is assisted through packing, a crack network is expanded, and the direction is controlled; bacillus type anaerobic bacteria are added into the fracturing fluid, micropores are opened, and a gas channel is established; and finally, the subsequent drilling position is determined through intelligent analysis, and slotting and fracturing are repeated. According to the method, a large-area fracture network with good continuity is formed, the permeability is improved, the gas extraction efficiency is remarkably improved, the limitations of fast fracture closing, random distribution, difficult pore forming and the like in the traditional technology are overcome, and the method is suitable for a deep coal seam with the ground stress of 20-40 MPa and the gas pressure of 1.5-3 MPa, safe to operate and environmentally friendly.

The invention provides a method for treating and recycling iron-containing waste sulfuric acid in titanium dioxide production, which is a method for treating iron-containing waste sulfuric acid on the basis of screening strains for treating iron-containing waste water in a low-pH environment, and can effectively realize resource recycling. The used wastewater treatment bacterium is a bacillus QD-QJ-062 strain, and the preservation number of the bacillus QD-QJ-062 strain is CGMCC (China General Microbiological Culture Collection Center) No. 33725. The screened bacillus QD-QJ-062 strain can be used for effectively treating iron-containing wastewater in a low-pH environment, the generation of ferrous oxalate precipitate is promoted, and a ferrous oxalate byproduct is generated. The generated ferrous oxalate can be converted into ferrous sulfate through sulfuric acid, oxalic acid is recycled, the operation cost is reduced, the sulfuric acid solution subjected to iron removal can be neutralized through limestone, high-purity gypsum is generated, and resource recycling treatment is achieved.

The invention discloses a multifunctional soil conditioner based on agricultural and forestry waste fermentation and a preparation method thereof, and relates to the field of soil conditioner preparation. The multifunctional soil conditioner based on agricultural and forestry waste fermentation is prepared from the following raw materials in parts by volume through two-stage fermentation: 60-80 parts of a fermentation main material, 10-25 parts of a functional auxiliary material, 5-15 parts of a complex microbial inoculant and 3-8 parts of a fermentation synergist, the fermentation main materials comprise seaweed residues, waste edible mushroom sticks and common agricultural and forestry wastes; the fermentation synergist is a compound of diatomite activated by a citric acid solution and humic acid raw powder; the complex microbial inoculant comprises a bacillus strain and a trichoderma strain in a specific ratio. Through the innovative compatibility of the seaweed residues and the waste mushroom sticks, the introduction of the fermentation synergist and the synergistic effect of the two-stage precise fermentation process, the defects in the prior art are effectively overcome, the fermentation efficiency and the product stability are remarkably improved, and efficient resource utilization of the agricultural and forestry residues is achieved.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention relates to a process for producing clean soil for planting from heavy metal-containing sludge, including the following steps: (i) Pre-treatment to remove large impurities from the sludge to obtain pre-treated sludge; (ii) Separation of heavy metals from the pre-treated sludge; (iii) Dehumidification and sterilization of harmful microorganisms in the sludge after heavy metal separation by high temperature; (iv) Mixing the sludge obtained from step (iii) with straw / rice husk, organic fertilizer, and microbial preparations containing Trichoderma spp. and Bacillus spp. to obtain clean soil for planting. The process described in the invention is environmentally friendly, low-cost, and easily applicable at the local level. The resulting clean soil meets agricultural soil standards, helps regenerate resources, and reduces pollution.

The invention relates to the technical field of organic solid waste resource utilization and microorganisms, and discloses a hermetia illucens sand staged matrix treatment method based on a special microbial agent and application of the hermetia illucens sand staged matrix treatment method. According to the method, hermetia illucens sand serves as a main treatment object, after the carbon nitrogen ratio and the water content are regulated and controlled, a special complex microbial inoculant composed of at least two microorganisms of bacillus, lactobacillus and azotobacter is inoculated, and under the condition that a continuous forced aeration device is not arranged, the hermetia illucens sand is subjected to aeration treatment for 20-30 min, and the hermetia illucens sand is obtained. The treatment process is divided into a microbial community construction stage and a low-disturbance stable transformation stage, and matrix treatment is completed through staged stirring regulation and control. According to the method, a microbial community establishment state is taken as a stage switching basis, high-frequency mechanical disturbance and continuous oxygen supply requirements are reduced, and conversion and humification of organic matters in the hermetia illucens sand and nitrogen stabilization are realized. And the obtained matrix product is stable in structure and relatively high in decomposition degree, and can be used for preparing a seedling substrate, an organic fertilizer or a soil conditioner.

The invention discloses a rice endophyte for endowing rice with low cadmium accumulation and rice blast resistance and application of the rice endophyte, and relates to the related technical fields of agricultural microorganisms, rice cadmium toxicity and rice disease control. The rice endophyte is bacillus, is named as bacillus velezensis # 16 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20252423. The rice endophyte provided by the invention is derived from a rice body, has high affinity with rice, can stably breed and grow in rice tissues, enhances the resistance of rice to rice blast, and reduces the accumulation of cadmium in rice grains; the strain can produce IAA and has the capability of promoting the growth and development of rice. The bacterium can be applied to cadmium-polluted and rice blast-damaged farmlands, is green and efficient in work, has disease-resistant and cadmium-reducing functions, can reduce the use of chemical pesticides and reduce the harm of environmental pollution to human bodies, and meets the development requirements of green agriculture.

The invention discloses a bacillus Bwzl 19 strain and an application of the bacillus Bwzl 19 strain. The bacillus sp. Bwzl 19 strain is separated from tobacco leaves, is preserved in Guangdong Microbial Culture Collection Center on November 8, 2024, has a preservation number of GDMCC NO: 65445, and is a new species of bacillus if the consistency of the whole genome average nucleotide of any reference strain in the bacillus does not reach a threshold value (gt, 95%) for judging that the reference strain is the same species. The Bwzl 19 strain does not carry virulence genes, can rapidly grow with starch as a unique carbon source and is high in nicotine tolerance, the content of starch in tobacco leaves can be reduced by spraying a fermentation culture solution of the Bwzl 19 strain to the tobacco leaves, and the Bwzl 19 strain can be used for improving the quality of the tobacco leaves. In addition, the Bwzl 19 strain can also effectively inhibit plant pathogenic fungi such as colletotrichum hijinensis and the like, and can be prepared into a microbial agent for preventing and treating related plant fungal diseases.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to a method for efficient secretory expression of pichia pastoris for recombinant bacillus lipase LIPBST and BSLA. The method comprises the following steps: respectively constructing recombinant secretory expression vectors containing LIPBST and BSLA mature peptide coding sequences, transforming the recombinant secretory expression vectors into a pichia pastoris host, and realizing efficient secretory expression of the two lipases by optimizing respective inducible expression conditions (the LIPBST is 2% v / v methanol concentration and induction start OD600 is equal to 3, and the BSLA is 1% v / v methanol concentration and induction start OD600 is equal to 1). Through fermentation culture and affinity chromatography purification, the high-purity recombinase is obtained, the LIPBST yield reaches 230 mu g / mL, and the enzyme activity is 195 U / mL; the yield of the BSLA reaches 125 mu g / mL, and the enzyme activity is 126 U / mL. The high-yield expression of the LIPBST and the BSLA is realized in the pichia pastoris for the first time, and the obtained enzyme preparation has excellent alkaline tolerance and medium-temperature activity and is suitable for the fields of detergents, food processing, biological catalysis and the like.

The application relates to the technical field of microbial remediation methods, and specifically discloses an ex-situ microbial remediation method for river sewage, aiming to solve the multiple contradictions of redundancy of existing ex-situ microbial remediation technology, single function of bacterial flora, weak environmental adaptability and difficulty in giving consideration to high efficiency and engineering simplicity. The method comprises the following steps: guiding the river sewage out and pretreating the river sewage to remove large-particle impurities; introducing a biological reactor to inoculate a composite functional bacterial flora composed of the genus Raoultella, the genus Pseudomonas and the genus Bacillus in proportion; implementing gradient dissolved oxygen regulation to realize simultaneous nitrification and denitrification; adding iron-manganese modified biochar as a microbial carrier and an electron mediator; and recycling the biochar after solid-liquid separation and reaction. Through the above technical scheme, the application can realize multi-target and simultaneous high-efficiency removal of ammonia nitrogen, refractory organic matter and trace heavy metals, simplify a process flow, improve system stability and low-temperature adaptability, and support modular deployment and intelligent operation and maintenance.

The present invention relates to the field of protein nanofibrils originating from Bacillus sp. provided in a composition as additive to a biologically active substance, in particular to a microbial substance such as a pesticide or plant beneficial agent. More specifically, the present invention relates to a composition comprising an agriculturally bioactive substance and Alpha(helical) Endospore appendage (A-ENA) protein nanofibril materials, more precisely wherein said A-ENA protein nanofibrils are recombinantly produced and exogenously added to said bioactive substance of the composition, thereby functioning as coformulant or additive, and preferably increasing the biological activity of said substance. In particular, the composition of the invention comprises B. thuringiensis spores or cells and self-assembled (engineered or functionalized) A-ENA protein nanofibrils as coformulant, providing for an improved efficacy and / or application potential as pesticide or insecticide. The present invention further relates to a method for controlling plant growth and protection of plants or parts thereof from pest damage, in particular by applying said composition of the invention in an effective amount or formulation, wherein the presence of said A-ENA protein nanofibril coformulant or additive results in increased efficacy and / or virulence of the bioactive substance of said composition, in particular for bioactive substances comprising Bacillus sp. strains.

The invention provides D-lactic dehydrogenase, an engineering strain containing the D-lactic dehydrogenase as well as construction and application of the D-lactic dehydrogenase. The invention discloses a d-lactic dehydrogenase (d-lactic dehydrogenase), which has unique properties and is derived from thermodesulfurization bacillus, and the d-lactic dehydrogenase has very good thermophilic property and thermal stability. The invention further discloses a preparation method of the d-lactic dehydrogenase. By utilizing the d-lactic acid dehydrogenase and the genetic engineering modification method, a fermentation product of the modified bacillus licheniformis is redirected from naturally produced 2, 3-butanediol to high-yield optically pure d-lactic acid, and the optical purity of the produced d-lactic acid reaches 99.9%; raw materials used for fermentation are low in price, and the fermentation state is between anaerobic fermentation and micro-aerobic fermentation. The method for producing d-lactic acid through high-temperature fermentation can save the cost and improve the production efficiency, and has a wide industrial application prospect.

This invention relates to the field of microbial self-assembled protein fibrils as novel bionanomaterials. More specifically, this invention relates to protein nanofibril materials comprising Bacillus endospore appendage (ENA) proteins, which are spontaneously folded helical hairpin subunits assembled into α-helical multimers (A-ENA) fibrils having a hydrophobic core and covalently linked by one or more isopeptide bonds, providing nanofibrils with high stability and tensile strength. This invention also relates to said protein nanofibrils being engineered or modified to provide functionalized bionanomaterials. In particular, this invention relates to methods for the recombinant preparation of said self-assembled protein nanofibrils, and their use in modifying bacterial endospores and endospore activity or pathogenicity.

The invention relates to the field of organic solid waste resourceful treatment, and provides a hermetia illucens solid waste conversion method based on intestinal pre-planting of exogenous microorganisms and synergistic effect. The method comprises the following steps: in an egg incubation stage or within 0-6 days after larva incubation, enabling eggs or larvae to eat an incubation / brooding substrate containing exogenous microorganisms to obtain larvae subjected to intestinal tract pre-planting treatment; and then inoculating the larvae into an organic solid waste matrix for biotransformation, feeding until at least 50% of the larvae enter a prepupal stage, and then collecting the larva biomass and the residual matrix. Paenibacillus or bacillus microorganisms with corresponding functions can be selected as exogenous microorganisms according to feeding requirements. The embodiment shows that the method can improve the larva biomass and the biological conversion rate, reduce the feed conversion ratio, and improve the survival rate and the polypide protein and grease yield.

This invention belongs to the field of biomedical and analytical technology and provides an early screening model for esophageal cancer based on oral microorganisms and a method for constructing the same. [Solution] The early screening model uses the oral microbiome, including Prevotella, Granulicatella, Rothia, Prevotellamassilia, Blautia, Abiotrophia, Peptostreptococcus, Actinomyces, Burkholderia, Akkermansia, Fudania, Kineothrix, Bacillus, Duncanella, and Eisenbergiella, as markers. By identifying non-invasive biomarkers, asymptomatic esophageal cancer patients can be detected early.

This invention discloses a strain of *Bacillus halophilus* and its application in promoting sesame growth and improving salt and alkali tolerance, belonging to the field of sesame cultivation technology. This strain of *Bacillus halophilus* is... Halobacillus sp. OCR1-S1, with the preservation number CCTCC NO:M 20252396. Experiments showed that inoculation with the OCR1-S1 strain during the sesame germination stage under salt-alkali stress significantly promoted the growth of fresh weight, root length, and shoot length in sesame buds; inoculation with the OCR1-S1 strain during the sesame seedling stage significantly enhanced the sesame's resistance to salt-alkali stress; simultaneously, treatment of sesame roots with the OCR1-S1 strain under normal soil conditions also promoted sesame plant growth and increased phosphorus content. This invention provides a new microbial resource and a reliable solution for sesame to cope with salt-alkali stress, and is of great significance for utilizing my country's large areas of saline-alkali land to meet the needs of sesame industry expansion.

This invention discloses a method for controlling root rot in Panax notoginseng based on a cross-species synthetic community and its application, belonging to the field of agricultural biological control. This cross-species synthetic community consists of a specific ratio of Bacillus bacteria and Trichoderma fungi. Biological agent capsules are prepared and applied as a basal soil treatment before planting. At the time of Panax notoginseng emergence, 5-10 capsules (equivalent to 100-200 mg per plant) are buried in each planting hole. The capsules slowly release the microbial community in the soil for 2-3 months. This synthetic community can significantly reduce the incidence of root rot in Panax notoginseng, promote plant growth, and increase the content of Panax notoginseng saponins. It achieves comprehensive control by inhibiting pathogens, reshaping the rhizosphere microecology, activating plant immunity, and regulating metabolism. It is suitable for green planting of Panax notoginseng and the management of continuous cropping obstacles, possessing both high efficiency and ecological safety.