Pediococcus pentosaceus and application thereof

A technology of Pediococcus pentosaceus and ribose, applied to Pediococcus pentosaceus and its application field, can solve problems such as limited application, and achieve the effects of improving liver function, reducing the degree of liver damage, and widening the antibacterial spectrum

Active Publication Date: 2014-01-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Pediococcus pentosaceus is mainly used for food fermentation and the currently used strains are mainly derived from food. There are few reports on the strains derived from human or animal guts, which limits the application of this strain in the field of animal and human health

Method used

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  • Pediococcus pentosaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1. Isolation and purification of Pediococcus pentosacea

[0035] 1) Take 0.5 grams of fresh feces samples from different healthy people, add them to 10 ml of Pediococcus pentosaceae liquid medium, mix well, and dilute 10,000 times with the above liquid medium. The composition of the Pediococcus pentosaceae liquid medium is: 5 grams of peptone, 3 grams of yeast extract, 5 grams of wort extract, 5 grams of glucose, 1.0 grams of Tween 80, 1.5 grams of dipotassium hydrogen phosphate, acetic acid trihydrate 2.0 grams of sodium, 1.0 grams of diammonium citrate, 0.2 grams of magnesium sulfate heptahydrate, 0.05 grams of manganese sulfate tetrahydrate, pH 6.2, 960 ml of distilled water.

[0036] 2) Take 100 microliters of the above dilution, spread it on the solid culture medium plate of Pediococcus pentosacea, at 37 0 C. Under anaerobic conditions, cultivate for 72 hours. The composition of the Pediococcus pentosaceae solid medium is: 5 grams of peptone, 3 grams...

Embodiment 2

[0039] Example 2. Screening of acid-tolerant Pediococcus pentosaceae

[0040] Prepare Pediococcus pentosaceae culture medium with pH 2.0, 2.5, 3.0, 4.0, 5.0, 6.4, inoculate Pediococcus pentosaceae that has been cultured for 18 hours according to 1%, at 37 0C. Under anaerobic conditions, culture for 0, 1, 2, 3 h, 24 h, dilute to an appropriate ratio, smear on a plate, culture for 24 counts, calculate and compare the growth of Pediococcus pentosaceae under different pH conditions. Pediococcus pentosacea Pediococcus pentosaceus LI05 etc. have good acid resistance and can grow at pH 4.0.

Embodiment 3

[0041] Example 3. Screening of bile salt-tolerant Pediococcus pentosaceae

[0042] Prepare Pediococcus pentosaceae culture medium containing 0.5%, 1.0%, 1.5%, 2.0%, and inoculate the Pediococcus pentosaceae bacterial liquid that has been cultivated for 18 hours, the inoculation amount is 1%, and at 37 0 C. Cultivate for 24 hours under anaerobic conditions. Observe the turbidity of the culture medium; at the same time, take cultures of different concentrations of bile salts, dilute them in an appropriate ratio, and smear them on a plate for counting. Pediococcus pentosacea Pediococcus pentosaceus LI05 has good anti-bile salt performance, and 2% bile salt has almost no effect on its growth rate.

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Abstract

The invention discloses pediococcus pentosaceus and application thereof. The Latin name of the pediococcus pentosaceus is Pediococcus pentosaceus LI05, and the pediococcus pentosaceus is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No.7049. The pediococcus pentosaceus disclosed by the invention has the morphological characteristics that the thallus is rod-shaped, dose not generate spores, does not have motility, and has masculine Gram staining. The whole-cell fatty acid of the pediococcus pentosaceus disclosed by the invention comprises the following main components in percentage by weight: about 1.45% of 14:0, about 3.26% of 16:1w7c/16:1w6c, about 31.25% of 16:0, about 6.31% of 18:1w9c, about 38.59% of 18:1w7c, about 1.37% of 18:0 and about 14.59% of un18.846/19:1w6c. The invention also discloses a complete sequence of 16S ribosome DNA (16SrDNA) of the pediococcus pentosaceus. The pediococcus pentosaceus and a preparation thereof disclosed by the invention can be used for adjusting the micro-ecological balance of intestinal tracts of human or animals, promoting digestive absorption and slowing down the happening and development of liver failure of experimental animals.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a Pediococcus pentosacea and application thereof. Background technique [0002] There are a large number of colonization in the human intestine (10 14 ), complex structure (more than 1000 species of bacteria) microbial community (approximately 1.5 kg). In the process of symbiosis and co-evolution between the intestinal flora and the host, it plays an important role in regulating the host's digestion and absorption, immune response, metabolism, etc., and has been paid more and more attention as the most important micro-ecosystem of the human body. More and more research results show that the intestinal microbiome plays an important role in the pathogenesis of various diseases, such as neonatal necrotizing colitis, asthma, eczema, inflammatory bowel disease, obesity, atherosclerosis, insulin Drug resistance and tumors etc. [0003] Lactic acid bacteria are a general term for a class ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P1/14A61P1/16A61P3/04A61P31/04A61P37/02C12R1/01A61K35/744
CPCY02A50/30
Inventor 李兰娟吕龙贤胡新俊钱贵荣
Owner ZHEJIANG UNIV
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