Ethinyl estradiol high-efficiency degrading strain and preparation method thereof

A technology for ethinyl estradiol and highly efficient degrading bacteria, which is applied in the field of estrogen degrading bacteria and its preparation, can solve problems such as inability to effectively remove, achieve the effect of simple and fast separation and purification methods, ensure ecological safety, and have broad application prospects

Inactive Publication Date: 2012-02-15
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ethinyl estradiol is an aromatic heterocyclic compound, and the current traditional sewage treatment methods (such as adsorption, activated sludge, biofilm, ultraviolet, photocatalytic degradation, etc.) cannot be effectively removed, so it is frequently detected and has Certain ecotoxicity, is currently one of the most concerned estrogenic pollutants

Method used

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  • Ethinyl estradiol high-efficiency degrading strain and preparation method thereof
  • Ethinyl estradiol high-efficiency degrading strain and preparation method thereof
  • Ethinyl estradiol high-efficiency degrading strain and preparation method thereof

Examples

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Embodiment 1

[0026] (1) The activated sludge taken from the secondary settling tank of the urban sewage treatment plant is inoculated in the inorganic salt medium by 10% of the access amount, wherein the inorganic salt medium is K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 Cl 2.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.03g, distilled water 1000mL, pH=6.8-7.0. Acclimate by gradient pressure acclimation method, the concentration of ethinyl estradiol is 3, 5, 10, 15, 20, 25, 30mg / L, under the conditions of 30°C, 160r / min, and 100mL filling volume in a 250mL Erlenmeyer flask Domestication and cultivation, the total domestication time is 2 months;

[0027] (2) Take the sample after 2 months of acclimation, let it stand for 30 minutes, take 1mL of the upper layer water sample, and make 10 -1 ~10 -10 Gradient dilution. For each dilution concentration, take 1 mL of the bacterial solution into a petri dish, pour the enriched medium into it, put the petri dish on...

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Abstract

The invention relates to an ethinyl estradiol high-efficiency degrading strain and a preparation method thereof. The thallus length of the strain is 0.4-0.8um; and the bacterial colony has a diameter of 1-2.5mm, is round in shape, has regular edges and a low convex surface, is golden yellow in color with no luster and no transparency, and appears as negative through Gram staining. The preparation method comprises the following steps: carrying out directional domestication and culture on active sludge, enriching, separating, and purifying to obtain the ethinyl estradiol high-efficiency degrading strain. The ethinyl estradiol high-efficiency degrading strain can effectively degrade ethinyl estradiol, has an ethinyl estradiol degradation rate up to 80% or above, has no resistance to common antibiotics, and can be inactivated easily, thereby ensuring the ecological safety in use. Besides, the ethinyl estradiol high-efficiency degrading strain has the advantages of easy acquisition of sources and convenient separation and purification method.

Description

technical field [0001] The invention belongs to the field of estrogen-degrading bacteria and its preparation, in particular to a highly efficient ethinyl estradiol-degrading bacteria and its preparation method. Background technique [0002] With the improvement of environmental analysis technology and the enhancement of people's environmental awareness, synthetic estrogen, as a new pollutant, has attracted more and more attention. Although the concentration of such substances in the environment is not high, even a small amount of estrogens will cause great harm to the environment and human health. [0003] There are many types of synthetic estrogens reported, ethinyl estradiol (17α-ethynylestradiol, EE2) (molecular structure such as figure 1 shown) as an active ingredient in many contraceptives has been frequently detected in lakes, rivers and even soil around the world in recent years. However, ethinyl estradiol is an aromatic heterocyclic compound, and the current tradit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 陈东辉黄敏黄满红韩潇丁丽娜孙彦刘冲刘鹏程张泽光肖斌夏昆
Owner DONGHUA UNIV
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