Method for extracting iopromide efficient degrading bacterium
A technology for efficiently degrading bacteria and iopromide, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of drinking water safety hazards, non-removal, entry into surface runoff, groundwater, and even drinking water
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[0055] (1) The activated sludge taken from the secondary settling tank of the urban sewage treatment plant is inoculated in the inorganic salt medium by 5% of the access amount, wherein the inorganic salt medium is K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 Cl 2.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.03g, distilled water 1000mL, pH=6.8-7.0. Acclimate by gradient pressure acclimatization method, iopromide concentration is 10, 50, 80, 100, 130, 150mg / L, at 30°C, 160r / min, 150mL Erlenmeyer flask filling capacity of 100mL shaker culture conditions The total domestication time is 2 months.
[0056] (2) Take the sample after 2 months of acclimation, let it stand for 30 minutes, take 1mL of the upper layer water sample, and make 10 -1 ~10 -10 Gradient dilution. Take 1mL of bacterial solution for each dilution concentration in a petri dish, then take 1mL of bacterial solution for each dilution concentration and spread it on a solid medium, and ...
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