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Method for extracting iopromide efficient degrading bacterium

A technology for efficiently degrading bacteria and iopromide, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of drinking water safety hazards, non-removal, entry into surface runoff, groundwater, and even drinking water

Inactive Publication Date: 2012-01-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, iopromide is a nitrogen-containing aromatic heterocyclic compound, and the conventional process of urban sewage treatment plants has almost no removal effect on it, so that it enters surface runoff, groundwater, and even drinking water with the effluent of sewage treatment plants
Some European countries such as Germany have frequently detected iopromide in various water bodies, sometimes with concentrations as high as 10 μg / L, causing potential harm to drinking water safety

Method used

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  • Method for extracting iopromide efficient degrading bacterium
  • Method for extracting iopromide efficient degrading bacterium
  • Method for extracting iopromide efficient degrading bacterium

Examples

Experimental program
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specific Embodiment approach

[0055] (1) The activated sludge taken from the secondary settling tank of the urban sewage treatment plant is inoculated in the inorganic salt medium by 5% of the access amount, wherein the inorganic salt medium is K 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 Cl 2.1g, MgSO 4 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.01g, CaCl 2 2H 2 O 0.03g, distilled water 1000mL, pH=6.8-7.0. Acclimate by gradient pressure acclimatization method, iopromide concentration is 10, 50, 80, 100, 130, 150mg / L, at 30°C, 160r / min, 150mL Erlenmeyer flask filling capacity of 100mL shaker culture conditions The total domestication time is 2 months.

[0056] (2) Take the sample after 2 months of acclimation, let it stand for 30 minutes, take 1mL of the upper layer water sample, and make 10 -1 ~10 -10 Gradient dilution. Take 1mL of bacterial solution for each dilution concentration in a petri dish, then take 1mL of bacterial solution for each dilution concentration and spread it on a solid medium, and ...

Embodiment 2

[0061]

[0062]

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Abstract

The invention relates to a method for extracting an iopromide efficient degrading bacterium, which comprises the steps of: carrying out directional acclimatization culture on return sludge of a secondary sedimentation tank of a municipal sewage treatment plant, and then carrying out enrichment separation and purification on a sample subjected to acclimatization culture to obtain the iopromide efficient degrading bacterium. A provenance for extracting the iopromide efficient degrading bacterium is active sludge of the municipal sewage treatment plant, is easy to obtain, and is convenient and rapid for separating and purifying. The iopromide efficient degrading bacterium extracted by the invention can effectively degrade iopromide under the condition of co-metabolism, is easy to inactivate, and is inactivated after being irradiated by ultraviolet rays for 2 hours, thus ecological safety of the use is ensured.

Description

technical field [0001] The invention belongs to the field of extracting degrading bacteria of nitrogen-containing aromatic heterocyclic compounds, and in particular relates to an extracting method of highly efficient iopromide degrading bacteria. Background technique [0002] In recent years, pharmaceuticals and personal care products (PPCPs) have become new trace pollutants that have attracted widespread attention at home and abroad. Although the concentration of such substances in the environment is very low, and some of them usually exist at the level of ng / L~μg / L in the environment, considering the cumulative effect of pollutants in the environment, it cannot be ruled out that their adverse effects on the environment influences. [0003] Among common PPCPs compounds, antibiotics, anti-inflammatory drugs, antiepileptic drugs, and iodinated contrast agents were detected in various environmental media. Iopromide (molecular structure such as figure 1 As shown), it is an i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/38A62D101/28
Inventor 刘亚男刘凯英薛罡刘振鸿石为民杨琦吴瑶冯菲李姗珊杨萍刘琼
Owner DONGHUA UNIV
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