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Method for enhancing 4-valent epidemic meningococcal polysaccharide protein bonder immunogenicity

An epidemic meningitis, polysaccharide protein technology, applied in the direction of carrier binding antigen/hapten components, antibacterial drugs, pharmaceutical formulations, etc., can solve problems such as weak immunogenicity

Active Publication Date: 2014-10-15
KANVAX BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The clinical application of the 4-valent meningococcal polysaccharide conjugate vaccine has achieved results, however, some groups are still weakly immunogenic and need to be updated with the development of more immunogenic products

Method used

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  • Method for enhancing 4-valent epidemic meningococcal polysaccharide protein bonder immunogenicity
  • Method for enhancing 4-valent epidemic meningococcal polysaccharide protein bonder immunogenicity
  • Method for enhancing 4-valent epidemic meningococcal polysaccharide protein bonder immunogenicity

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Embodiment Construction

[0031] The specific implementation method of the present invention is illustrated below, but not limited to the following examples.

[0032]The present invention uses the method of gene recombination to express and purify the CRM197A protein carrier and the CRM197A protein carrier with the universal epitope peptide P2 in the expression system of Escherichia coli engineering bacteria; Polysaccharides (Men for short) were covalently linked to the P2CRM197A protein carrier to prepare a Men‐P2CRM197A conjugate; after mixing four monovalent polysaccharide-protein conjugates to prepare a vaccine, mice were immunized, and blood was collected to obtain immune serum; detected by ELISA Polysaccharide-specific antibody titers in mouse sera to assess the immunogenicity of polysaccharide-protein conjugates.

[0033] Step 1: Preparation of protein carrier and meningococcal capsular polysaccharide

[0034] In order to illustrate the effectiveness of the present invention, two carrier protei...

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Abstract

The invention discloses a method for enhancing 4-valent epidemic meningococcal polysaccharide protein bonder immunogenicity, and the method is as follows: adding all-powerful epitope peptide (P2) into CRM197A (A chain of diphtheria toxin variant 197), using genetic recombinant Escherichia coli to produce a P2-containing protein carrier P2CRM197A of the A chain of the diphtheria toxin variant 197 (CRM197); and connecting 4 different serogroups (comprising A, C, W135 and Y) capsular polysaccharides to the P2-containing protein carrier P2CRM197A by covalent bonds to form a 4-valent epidemic meningococcal polysaccharide-P2CRM197A bonder; compared with a 4-valent epidemic meningococcal polysaccharide-CRM197A bonder obtained by a corresponding protein carrier CRM197A which does not contain the all-powerful epitope peptide (P2), the immunogenicity of the 4-valent epidemic meningococcal polysaccharide-P2CRM197A bonder obtained by the method is increased by 3-5 times than that of a contrast.

Description

technical field [0001] The invention relates to a method for enhancing the immunogenicity of a polysaccharide-protein conjugate of 4-valent epidemic meningococcus. Background technique [0002] When the polysaccharide is covalently linked to the protein carrier, the polysaccharide of the hapten can be converted into a whole antigen, so that the immunogenicity of the polysaccharide is enhanced. The polysaccharide-protein conjugated vaccine synthesized by this method has been widely used in children, successfully preventing bacterial infections including pneumococcus, meningococcus and Haemophilus influenzae type b. [0003] There are many protein carriers used to synthesize polysaccharide-protein conjugates, such as tetanus toxoid, diphtheria toxoid, diphtheria toxin variant CRM197, and Haemophilus epidemic surface protein D produced by genetic recombination technology; however, due to different The immunogenicity of the protein, the immunogenicity of polysaccharide-protein ...

Claims

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Application Information

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IPC IPC(8): A61K39/385A61K47/48A61P31/04
Inventor 李建平
Owner KANVAX BIOPHARM
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