Preparation method of group A/C meningococcal polysaccharide

A technology of meningococcal and capsular polysaccharides, applied in the field of biomedicine, can solve the problems of phenol environmental hazards, water and air pollution, and achieve the effects of avoiding large-scale use, avoiding environmental damage, and preventing phenol from damaging the environment

Inactive Publication Date: 2017-02-01
CHENGDU OLYMVAX BIOPHARM
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, phenol has serious harm to the environment

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  • Preparation method of group A/C meningococcal polysaccharide
  • Preparation method of group A/C meningococcal polysaccharide

Examples

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Embodiment 1

[0023] Preparation of Group A Meningococcal Polysaccharide

[0024] Open a working seed batch of group A meningococcus, and inoculate it into two test tubes of meningococcal semi-comprehensive medium at a temperature of 35-37°C and cultivate for 16-24 hours, which is the first-generation strain. Each first-generation strain is propagated on 2 bottles of meningococcal semi-comprehensive medium at a temperature of 35-37°C for 16-24 hours, which is the second-generation strain. Inoculate each bottle of second-generation strains on 6 bottles of meningococcal semi-comprehensive medium at a temperature of 35-37°C for 16-24 hours to form third-generation strains. The three generations of bacteria were collected in 500ml of physiological saline solution and mixed well, and then inoculated in a 50L seed tank at a temperature of 35-37°C and a culture time of 4 hours. Then, 50L seed tank culture was inoculated into 500L fermenter for culture, the temperature was 35~37℃, and after 8 hours o...

Embodiment 2

[0029] Preparation of Group C Meningococcal Polysaccharide

[0030] Open 1 working seed batch of group C meningococcus and inoculate it into 2 test tubes of meningococcal semi-comprehensive medium at a temperature of 35~37℃ and cultivate for 16~24 hours, which is the first generation of bacteria. Each first-generation strain is propagated on 2 bottles of meningococcal semi-comprehensive medium at a temperature of 35-37°C for 16-24 hours, which is the second-generation strain. Inoculate each bottle of second-generation strains on 6 bottles of meningococcal semi-comprehensive medium at a temperature of 35-37°C for 16-24 hours to form third-generation strains. The three generations of bacteria were collected in 500ml of physiological saline solution and mixed well, and then inoculated in a 50L seed tank at a temperature of 35-37°C and a culture time of 4 hours. Then, 50L seed tank culture was inoculated into 500L fermenter for culture, the temperature was 35~37℃, and after 8 hours ...

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Abstract

The invention discloses a preparation method of group A/C meningococcal polysaccharide. The preparation method comprises the following steps: (1) carrying out fermentation culture on meningococcus, and carrying out sterilization treatment, thus obtaining a sterilized culture solution; (2) preparing meningococcus capsular polysaccharide; and (3) dissolving the rough polysaccharide, carrying out chromatography elution, collecting target peaks containing the polysaccharide, carrying out desalting on the collected polysaccharide solution by adopting an ultrafiltration membrane and adopting water for injection as an ultrafiltrate, thus obtaining the desalted solution, namely, the group A/C meningococcal polysaccharide solution. According to the preparation method, firstly, sterilization treatment is carried out, so that the inactivated culture solution is obtained, then, the capsular polysaccharide is prepared, and then, the capsular polysaccharide is purified by adopting the chromatography method; the use of abundant phenol in the traditional purification method is avoided, on the basis that the purification quality is guaranteed, the destruction of phenol to the environment is effectively avoided, meanwhile, the recovery rate of polysaccharide is approximate to that of the traditional phenol extraction method, in addition, the operation is simple, and the preparation method is suitable for large-scale production.

Description

Technical field [0001] The invention relates to the field of biomedicine, and more specifically to a method for preparing group A and C meningococcal polysaccharides. Background technique [0002] Meningococcal meningitis (meningococcal meningitis for short, the same below) is an acute respiratory infection caused by Neisseria meningitidis. For more than one hundred years, it has been circulating or sporadic all over the world, and infection with pathogenic bacteria can cause sepsis and meningitis. The susceptible population is mainly children, with the highest fatality rate of fulminant type, which can reach 40% to 60%. Today, the incidence rate on all continents of the world is 1 / 100,000 to 10 / 100,000, and the total case fatality rate is 5% to 15%. Up to 20% of meningitis patients will have neurological sequelae, including mental impairment and deafness. According to the type of capsular polysaccharide, the serological classification can be divided into 13 serotypes, among wh...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00C12R1/36
CPCC08B37/0003C12P19/04C12N1/205C12R2001/36
Inventor 吴强马礼耕钟正丹
Owner CHENGDU OLYMVAX BIOPHARM
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