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ACY W135 group meningococcal polysaccharide type-b haemophilus influenzae conjugate vaccine

A technology of Haemophilus influenzae and meningococcus, applied in the direction of antibacterial drugs, bacterial antigen components, antibody medical components, etc., to achieve the effect of preventing epidemic meningitis, reducing endotoxin content, and reducing pain

Inactive Publication Date: 2009-12-02
长春长生生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The incidence of meningococcal meningitis in my country is mainly group A, so the vaccines developed are all group A or A+C bivalent vaccines. However, epidemic meningitis broke out in Guizhou in 2000 and Hebei in 2004, causing panic in the local area. Epidemiological surveys proved that the strains that caused the epidemic were group C strains, and W strains were once isolated in some areas. 135 Groups and Y groups, although not yet epidemics, have also attracted the attention of disease control departments

Method used

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  • ACY W135 group meningococcal polysaccharide type-b haemophilus influenzae conjugate vaccine
  • ACY W135 group meningococcal polysaccharide type-b haemophilus influenzae conjugate vaccine
  • ACY W135 group meningococcal polysaccharide type-b haemophilus influenzae conjugate vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 200901 Batch ACYW 135 Preparation of Group Meningococcal Polysaccharide Vaccine-Haemophilus Influenza Type B Conjugated Vaccine

[0049] 1. Take group A meningococcus 29201 strain, C group meningococcus 29205 strain, Y group meningococcus 29028 strain, W 135 Group meningococcus 29037 strain, inoculated on ordinary agar medium containing 10% sheep blood, meningococcus does not grow at 25 ℃. Cultivate in a carbon dioxide environment at 35-37°C for 16-20 hours, grow smooth, moist, off-white colonies, the bacterial lawn is easy to remove, and appears a uniform suspension in physiological sodium chloride solution. Staining microscopic examination should show Gram-negative diplococci and monococci.

[0050]2. Use culture tanks for liquid culture. During the cultivation process, samples were taken for pure bacteria inspection, and smears were used for Gram staining microscopy. If any contaminated bacteria were found, they should be discarded. The culture was terminated at ...

Embodiment 2

[0060] 200902 Batch ACYW 135 Preparation of Group Meningococcal Polysaccharide Vaccine-Haemophilus Influenza Type B Conjugated Vaccine

[0061] 1. Take group A meningococcus 29201 strain, C group meningococcus 29205 strain, Y group meningococcus 29028 strain, W 135 Group meningococcus 29037 strain, inoculated on ordinary agar medium containing 10% sheep blood, meningococcus does not grow at 25 ℃. Cultivate in a carbon dioxide environment at 35-37°C for 16-20 hours, grow smooth, moist, off-white colonies, the bacterial lawn is easy to remove, and appears a uniform suspension in physiological sodium chloride solution. Staining microscopic examination should show Gram-negative diplococci and monococci.

[0062] 2. Use culture tanks for liquid culture. During the cultivation process, samples were taken for pure bacteria inspection, and smears were used for Gram staining microscopy. If any contaminated bacteria were found, they should be discarded. The culture was terminated at...

Embodiment 3

[0072] 200903 Batch ACYW 135 Preparation of Group Meningococcal Polysaccharide Vaccine-Haemophilus Influenza Type B Conjugated Vaccine

[0073] 1. Take group A meningococcus 29201 strain, C group meningococcus 29205 strain, Y group meningococcus 29028 strain, W 135 Group meningococcus 29037 strain, inoculated on ordinary agar medium containing 10% sheep blood, meningococcus does not grow at 25 ℃. Cultivate in a carbon dioxide environment at 35-37°C for 16-20 hours, grow smooth, moist, off-white colonies, the bacterial lawn is easy to remove, and appears a uniform suspension in physiological sodium chloride solution. Staining microscopic examination should show Gram-negative diplococci and monococci.

[0074] 2. Use culture tanks for liquid culture. During the cultivation process, samples were taken for pure bacteria inspection, and smears were used for Gram staining microscopy. If any contaminated bacteria were found, they should be discarded. The culture was terminated at...

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Abstract

The invention provides an ACY W135 group meningococcal polysaccharide type-b haemophilus influenzae conjugate vaccine. A frozen-dry ACY W135 meningococcal polysaccharide vaccine is prepared by using the technology of removing endotoxin through ultracentrifugation so as to ensure that the endotoxin content of ACY W135 group meningococcal polysaccharide stock solution is greatly reduced before centrifugation. The conjugate vaccine can prevent disease infection of epidemic encephalitis for a patient by one dosage of injection, reaches and exceeds the preventing effect of the prior five dosages of injection, reduces the pain of the patient, and lowers the prevention cost.

Description

Technical field: [0001] The present invention provides an ACY W 135 The meningococcal polysaccharide-type b Haemophilus influenzae conjugate vaccine is used for the immune prevention of important cerebrospinal meningitis diseases, and belongs to the technical field of vaccine production and preparation. Background technique: [0002] The main incidence of meningococcal meningitis (meningococcal meningitis) is infants and young children, ACY W 135 Meningococcal polysaccharide vaccines are mainly used for the prevention of children, especially infants. In the 1980s, with the successful development of meningococcal polysaccharide vaccines and their inclusion in immunization programs, the number of people vaccinated worldwide has increased year by year. The incidence of meningococcal meningitis in my country is mainly group A, so the vaccines developed are all group A or A+C bivalent vaccines. However, epidemic meningitis broke out in Guizhou in 2000 and Hebei in 2004, causing ...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61K39/095A61K39/102A61P31/04
Inventor 杨宗辉孙惠军牟盈盈梁艳婷李冲巨爽
Owner 长春长生生物科技股份有限公司
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