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Epidemic encephalitis B/forest encephalitis hybrid virus and application of virus

A technology of forest encephalitis virus and forest encephalitis, applied in the field of genetic engineering, achieves good application prospects, high safety, and stable attenuation characteristics

Inactive Publication Date: 2013-10-16
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, cases of encephalitis caused by inoculation of YF-17D vaccine strains have occurred from time to time, indicating that the safety of chimeric vaccines based on this vaccine needs to be further improved

Method used

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  • Epidemic encephalitis B/forest encephalitis hybrid virus and application of virus
  • Epidemic encephalitis B/forest encephalitis hybrid virus and application of virus
  • Epidemic encephalitis B/forest encephalitis hybrid virus and application of virus

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1, constructing the Japanese encephalitis / forest encephalitis chimeric virus with the attenuated strain of Japanese encephalitis virus as the gene backbone

[0042] In this example, the attenuated JE virus SA14-14-2 strain is used as the gene backbone to construct the JE / forest encephalitis chimeric virus, wherein the cDNA sequence corresponding to the genomic RNA of the JE virus attenuated strain SA14-14-2 As shown in sequence 4 in the sequence listing, the 1st-95th position from the 5' end is 5'UTR; the 96th-476th position is the coding gene of capsid protein C (C); the 477th-2468th position is the deletion of E The gene encoding the prM-E protein with 3 amino acid residues at the carboxy-terminal of the protein (prM-EΔ3); the 2469-2477th position is the 3-amino acid residue encoding gene at the carboxy-terminal end of the E protein (E3); the 2478th-10394 The position is the coding gene (NS) of the non-structural protein NS; the 10395-10977 position is the ...

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Abstract

The invention discloses an epidemic encephalitis B / forest encephalitis hybrid virus and an application of the virus. The epidemic encephalitis B / forest encephalitis hybrid virus takes an epidemic encephalitis virus attenuated strain as a gene skeleton, and is a recombinant virus obtained by replacing a deoxyribonucleotide fragment of an encoded epidemic encephalitis virus prM-E delta 3 protein in epidemic encephalitis virus genome RNA (Ribonucleic Acid) with a deoxyribonucleotide fragment of an encoded forest encephalitis virus prM-E delta 3 protein in forest encephalitis virus genome RNA. The epidemic encephalitis B / forest encephalitis hybrid virus has the advantages that the virus is high in safety, and strong in stability, and can be induced to generate a good immune response to a forest encephalitis virus envelope protein, and protect an animal from being attacked by the exogenous forest encephalitis virus of lethal dose. The epidemic encephalitis B / forest encephalitis hybrid virus has a good application prospect in preventing and / or treating forest encephalitis virus infection as a vaccine.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and relates to a JE / forest encephalitis chimeric virus and its application, in particular to a JE / forest encephalitis chimeric virus with an attenuated JE virus strain as a gene skeleton and its application . Background technique [0002] Forest encephalitis virus, also known as tick-borne encephalitis virus (TBEV), is the pathogen of forest encephalitis. The virus is related to Yellow Fever virus (YFV), Japanese Encephalitis virus (JEV), Dengue virus (DENV) and West Nile virus (TBEV). are important members of the genus Flavivirus in the family Flaviviridae. Forest encephalitis virus is transmitted through tick bites and can cause neurological diseases after infecting humans. The virus is divided into European type, Siberian type and Far Eastern type, and the fatality rate of the Far Eastern subtype is as high as 30-40%. Forest encephalitis has become the most serious tick-borne viral disea...

Claims

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Application Information

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IPC IPC(8): C12N7/01A61K39/12A61P31/14C12Q1/70C12N15/85
CPCY02A50/30
Inventor 秦成峰李晓峰王洪江邓永强赵慧
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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