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95results about How to "Reduced risk of cross-contamination" patented technology

Method for constructing double-stem-loop structure DNA template to detect nucleic acid based on ligation reaction

The invention discloses a method for constructing a double stem loop structure DNA template to detect nucleic acid based on a ligation reaction. The method includes the steps that ligase is used for connecting a probe containing a stem-loop structure and complemented with nucleic acid to be detected to form a double-stem-loop structure DNA template, the template can guide fast and efficient loop-mediated isothermal amplification reaction to achieve high-sensitivity nucleic acid detection, and meanwhile the method can specifically distinguish nucleic acid with single-base difference. It is provided for the first time that double stem loop structure DNA is constructed through the high-specificity ligation reaction, the specificity template is provided for the loop-mediated isothermal amplification reaction in the next step, fluorescence labeling is not needed in the method, cost is low, the precise heat cycle process in the PCR process is avoided, and fast amplification of the nucleic acid to be detected can be realized at constant temperature. The method can be used for quantitative analysis, methylation detection, SNP detection and the like on RNA or DNA and provides a new strategy for high-sensitivity nucleic acid analysis, early cancer diagnosis and other researches.
Owner:SHAANXI NORMAL UNIV

Oxaliplatin lyophilized powder injection and preparing method thereof

The invention relates to oxaliplatin freeze-dried injection, which is characterized in that the invention is prepared by the method that aqueous solution is freeze-dried. The aqueous solution contains oxaliplatin, mannitol and citrate, wherein, the concentration of the oxaliplatin in the aqueous solution is 2.5 to 6.25 mg / ml; the concentration of the mannitol in the aqueous solution is 25 to 200 mg / ml; and the concentration of the citrate in the aqueous solution is 2 to 20 mg / ml. And sodium citrate can also be added into the aqueous solution so as to adjust the pH of the aqueous solution. The preparation processes are as following: the oxaliplatin is placed inside the container, 80 percent amount of water for injection is added, and then the water for injection is mixed so that the oxaliplatin can be dissolved and mixed evenly in the water for injection; after that, the mannitol and the citrate are added into the water for injection, and then the water for injection is mixed so that the mannitol and the citrate can be dissolved and mixed evenly in the water for injection; the content of the intermediate is measured; if the content of the intermediate is qualified, the volume of the water for injection is fixed to full amount; in the aseptic condition, the water for injection is filtered until to be clear by a microporous membrane of 0.22 microns; the filtered solution is filled into an aseptic silin bottle; part of the aseptic silin bottle is plugged with a butyl rubber closure; and then the aseptic silin bottle is filled in the tray to be sent into the freeze dryer to be freeze-dried; the mouth of the aseptic silin bottle is rolled; the quality of the filtered solution is inspected; and the aseptic silin bottle is packaged.
Owner:JIANGSU AOSAIKANG PHARMA CO LTD

Plate chain type conveyor

The invention discloses a plate chain type conveyor. The plate chain type conveyor comprises racks, two pairs of chain wheels supported at the two ends of the racks, and conveyor belts which are wound on the two pairs of chain wheels, wherein each conveyor belt is composed of two conveying chains and one group of material plate. The plate chain type conveyor is characterized in that a support lug is arranged at one edge, close to a chain plate on one side of the material plate, of each of the two conveying chains in a manner of extending outwards; a connection hole is formed in one front end of each support lug along the movement direction of the conveying chains, and a spring pin is arranged at the other end of each support lug; the spring pins are fixedly arranged on the outer sides of the support lugs and nail rods of the pins penetrate through the support lugs to enter the inner sides of the support lugs; when the conveying chains are at a stretched state, the distances from each connection hole to the adjacent two spring pins are equal; when the conveyor belts are located on the upper sides of the chain wheels, one end of each material plate is hinged with the connection holes in the support lugs of one pair of chain sections, and the other end of each material plate is supported on one rear spring pin adjacent to the connection holes. Unloading control devices are arranged on the rack close to the rail ends of the upper and lower layers of conveyor belts respectively.
Owner:GUANGZHOU UNIVERSITY

Blood collection tube labeling and assembling automatic connecting device

ActiveCN104150206ARealize automatic and orderly transmissionReduce direct contactConveyor partsBlood Collection TubeUltimate tensile strength
The invention discloses a blood collection tube labeling and assembling automatic connecting device which comprises a transfer device A (1), a lifting section (2), a transfer device B (3) and a reverse removing section (4). Each transfer device comprises a carrying platform and a rotating disc, wherein vacuum material suction devices are evenly distributed on the lower surface of the rotating disc circumferentially, and the rear end of the carrying platform is located at the lower portion of the rotating disc. The transfer device A (1) conveys materials outputted by a labeling machine (6) to a discharge bin A (7). The discharge port of the discharge bin A (7) is located at the upper portion of a lifting conveying belt (8). The transfer device B (3) conveys materials outputted by the lifting conveying belt (8) to the reverse removing section (4) and a horizontal conveying belt (12). A detecting device (14) and a removing device (15) are arranged on one side of the horizontal conveying belt (12). The blood collection tube labeling and assembling automatic connecting device has the advantages that full automation is achieved in the production process from labeling to assembling, personnel for arranging, transferring and placing products are omitted, work efficiency is improved, labor intensity is lowered, and production cost is saved.
Owner:江西科伦医疗器械制造有限公司

Method for suspending or re-suspending particles in a solution and apparatus adapted thereto

A method for suspending or re-suspending magnetically attractable particles is provided. Thereby at least a mixing vessel (10) is provided filled at least partially with a mixture (30) containing magnetically attractable particles (40) at least partially precipitated at the bottom (11) of the mixing vessel (10). An effective magnetic field acting at least in the front end area (3) of the mixing bar (1) is switched on by the magnetic field generating apparatus (4) while the mixing bar (1) is immersed in the mixture (30). Subsequently, the magnetic field is moved away from the bottom (11) of the mixing vessel (10) along with the mixing bar, whereby the movement of the magnetic field along with the mixing bar is carried out such that at least a part of the magnetically attractable particles (40) is raised from the bottom (11) of the mixing vessel (10) and the portion of the particles sticking to the bar is minimized. The magnetic field is switched off in a predefined distance from the bottom which is greater than the distance from the bottom at the time when the magnetic field is switched on. Thereafter, repeated mixing movements of the mixing bar (1) are carried out until the magnetically attractable particles present in the mixture (30) are sufficiently suspended or re-suspended whereby a magnetic field which is switched on does not exist at the front end (3) of the mixing bar (1).
Owner:QIAGEN GMBH

System and method for collinearly preparing multi-batch biological products

The invention relates to a system and a method for collinearly preparing multi-batch biological products. The system comprises a feed module, a discharge module, an inspection module, a transfer passage, a culture module and a plurality of liquid operating modules, wherein the feed module feeds raw materials and consumables required during preparation; the liquid operating modules separate and sort the raw materials and purify the raw materials before production; the culture module prepares cells; the inspection module detects and checks the prepared cells; the discharge module discharges thecells; the transfer passage transfers the cells among the feed module, the discharge module, the inspection module, the liquid operating modules and the culture module; air pressure control modules are arranged inside the feed module, the discharge module, the inspection module, the transfer passage, the culture module and the plurality of liquid operating modules respectively; the air pressure ofthe transfer passage is higher than those of the liquid operating modules. Through the system and the method, a cross-contamination risk during collinear production of non-finally sterilized biological preparations between different batches is lowered and the production efficiency is improved.
Owner:朱丹

Preparation method for single-phase crosslinked sodium hyaluronate gel

ActiveCN108395552AReduce the risk of useAvoid situations where it is difficult to mix evenlyPharmaceutical non-active ingredientsCross-linkButanediol
The invention discloses a preparation method for a single-phase crosslinked sodium hyaluronate gel. The method specifically comprises the following steps: sodium hyaluronate alkali treatment-a 1,4-butanediol diglycidyl ether cross-linking reaction-dialysate dialysis-preliminary granulation-and colloid mill grinding granulation. The method provided by the invention controls a crosslinking degree ofa crosslinking agent and HA through designing reaction conditions, so that a gel with suitable viscoelastic properties is obtained, a gel particle diameter suitable for secondary granulation of a colloid mill is obtained by subsequent preliminary crushing, and finally the crosslinked sodium hyaluronate gel with a target diameter is obtained by selection of appropriate colloid mill granulation parameters; and since the final product has suitable rheological properties, small particle sizes and uniform distribution, artificial operation in the production process can be reduced, the risk of cross contamination is reduced, the production filling process can be met without adding lubricants, the production cycle is shortened, the situation that two-phase cross-linked products are difficult tomix uniformly is avoided, the risk of clinical use is reduced, and product quality is improved.
Owner:ZHEJIANG JINGJIA MEDICAL TECH CO LTD

Oxaliplatin lyophilized powder injection and preparing method thereof

The invention relates to oxaliplatin freeze-dried injection, which is characterized in that the invention is prepared by the method that aqueous solution is freeze-dried. The aqueous solution contains oxaliplatin, mannitol and citrate, wherein, the concentration of the oxaliplatin in the aqueous solution is 2.5 to 6.25 mg / ml; the concentration of the mannitol in the aqueous solution is 25 to 200 mg / ml; and the concentration of the citrate in the aqueous solution is 2 to 20 mg / ml. And sodium citrate can also be added into the aqueous solution so as to adjust the pH of the aqueous solution. Thepreparation processes are as following: the oxaliplatin is placed inside the container, 80 percent amount of water for injection is added, and then the water for injection is mixed so that the oxaliplatin can be dissolved and mixed evenly in the water for injection; after that, the mannitol and the citrate are added into the water for injection, and then the water for injection is mixed so that the mannitol and the citrate can be dissolved and mixed evenly in the water for injection; the content of the intermediate is measured; if the content of the intermediate is qualified, the volume of the water for injection is fixed to full amount; in the aseptic condition, the water for injection is filtered until to be clear by a microporous membrane of 0.22 microns; the filtered solution is filled into an aseptic silin bottle; part of the aseptic silin bottle is plugged with a butyl rubber closure; and then the aseptic silin bottle is filled in the tray to be sent into the freeze dryer to be freeze-dried; the mouth of the aseptic silin bottle is rolled; the quality of the filtered solution is inspected; and the aseptic silin bottle is packaged.
Owner:JIANGSU AOSAIKANG PHARMA CO LTD

Prefilled injector for medicine dissolution and dispensing device

The invention discloses a prefilled injector for medicine dissolution and a dispensing device. The prefilled injector comprises a push rod, a piston, a needle tube and a protective cap, wherein the needle tube is provided with a medicine dissolution joint, the medicine dissolution joint is in matched connection with an external cone joint of a prefilled injector for injection, and the protective cap is in sealed connection to an opening of the medicine dissolution joint. During medicine dispensing, the protective cap of the prefilled injector for the medicine dissolution and a protective cap of the prefilled injector for injection are taken off, then, the medicine dissolution joint is directly in matched connection with the external cone joint of the prefilled injector for injection, pushrods of the two injectors are pushed repeatedly for medicine dispensing, after medicines are sufficiently dissolved, the prefilled injector for medicine dissolution is directly taken down, finally, the needle is mounted at the external cone joint of the prefilled injector for injection for injection. The scheme simplifies the dispensing process, the workload of a medical worker is reduced, and puncture scrap falling and cross pollution can be avoided. The invention also discloses the prefilled injector for injection and the dispensing device for the prefilled injector for medicine dissolution.
Owner:SHANDONG WEGO PREFILLS PHARM PACKAGING CO LTD

Automatic transmission cabinet

The invention relates to an automatic transmission cabinet. The automatic transmission cabinet comprises a cabinet body respectively provided with a front cabinet door and a back cabinet door at the front and back two ends; the front cabinet door and the back cabinet door have the same structure; the front cabinet door includes a front door frame, an automatic transmission line I and a front automatic door sheet; the upper end of the front automatic door sheet is hinged with the front door frame; the automatic transmission line I is arranged above a lower wall plate of the front door frame; a front electric pushing mechanism is fixedly mounted on an upper wall plate of the front cabinet door, and is connected with the upper end of the front automatic door sheet; and cabinet doors on two sides of the front electric pushing mechanism are respectively provided with a photoelectric induction device I and a photoelectric induction device II. The automatic transmission cabinet has the following beneficial effects: when materials are automatically transmitted, mutual isolation of two areas is realized, so that mutual air flowing between the areas is stopped, the risk of cross contamination is reduced, and the saving of energy is facilitated; the labor intensity is relieved; the pollution of external materials to indoor spaces is effectively prevented; and the cabinet doors can be singly used, and can be conveniently matched with other automatic production lines for use.
Owner:贺红宇
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