59 results about "Legionella pneumophila" patented technology

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention provides a kit for jointly detecting respiratory tract pathogen through a multiple fluorescent PCR method. The kit comprises six components: reaction liquid A, reaction liquid B, reaction liquid C, enzyme mixed liquid, positive control and negative control, and comprises 11 common respiratory tract pathogen detections (general type of influenza virus A, influenza virus B, respiratory syncytial virus, 1/2/3 type of human parainfluenza virus, adenovirus, mycoplasma pneumoniae, chlamydia pneumonia, legionella pneumophila, streptococcus pneumonia, haemophilus influenza, A streptococcal); the amplification is performed through three reaction buffers, and each reaction buffer contains four fluorescent channels, 90% pathogen infection on the clinic can be checked.

The invention provides a gene chip for detecting pathogens of lower respiratory tract, which comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises a sequence selected from one or more DNA sequences of staphylococcus aureus, 16s-23s rDNA intergenic spacer region of Klebsiella pneumoniae or Acinetobacter baumannii, 16S gene of pseudomonas aeruginosa or haemophilus influenzae, gyrB gene of streptococcus pneumoniae or legionella pneumophila and copB gene of moraxella catarrhalis or the complementary DNA or RNA sequence. The invention further provides a reagent kit containing the gene chip. The utilization of the gene chip and the reagent kit can detect the pathogens of the lower respiratory tract; furthermore, the operation is simple, the accuracy is high and the repeatability is strong.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia pneumoniae, haemophilus influenzae, mycoplasma pneumoniae, pneumoniae streptococcus and legionella pneumophila. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms (1) contacting said microorganisms of said sample with at least one repair compound and a growth medium, and (2) incubating the product of steps (1), and (3) detecting and quantifying said viable microorganisms, in which the microorganisms are of the species Legionella pneumophila, and in which the repair compound directly or indirectly causes an effect on the metabolism to reduce the oxidative stress of the microorganism. The invention also includes a kit for more accurately detecting and enumerating viable microorganisms of the species Legionella pneumophila in a sample suspected of containing said microorganisms.

The invention provides a method and kit for detecting multiple high-pathogenicity pathogenic bacteria, particularly a method for simultaneously detecting multiple high-pathogenicity pathogenic bacteria, which comprises the following steps: in a polymerase reaction system, carrying out PCR (polymerase chain reaction) on high-pathogenicity pathogenic bacteria by using a specific primer set, thereby obtaining an amplification product; and detecting with a specific probe or probe microsphere. The invention also provides a corresponding kit. The invention can sensitively and conveniently detect and identify multiple high-pathogenicity pathogenic bacteria, including Bacillus anthracis, Yersinia pestis, Clostridium botulinum, Brucella, Streptococcus suis, Vibrio cholerae, Francisella tularensis, Pseudomonas mallei (or Pseudomonas pseudomallei), Coxiella burnetii and Legionella pneumophila.

The invention discloses a quick detection kit for legionella pneumophila. A pair of legionella specific primers is used for amplifying a region on a 16S rRNA (ribonucleic acid) gene; a pair of hybridization probes is designed in the amplified fragment aiming at a legionella pneumophila specific sequence; an FAM fluorophore is labeled at an anchoring probe end 3'; a BHQ1 is labeled at a quenching probe end 5'; the end 3' is phosphorylated; the legionella pneumophila in a sample is detected through real-time fluorescence PCR (polymerase chain reaction) dissolving curve analysis. The detection kit mainly comprises a DNA (deoxyribonucleic acid) extracting solution, a fluorescence PCR reaction solution, a negative contrast and a positive contrast. The detection method comprises three steps of DNA extraction, fluorescence PCR reaction detection and dissolving curve analysis. The detection kit is operated simply and quickly, intuitive in result, high in sensitivity, high in specificity and high in stability and can be widely applied to quick identification of the legionella pneumophila in clinical and environment water samples.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

Provided are a method and a kit for accurately and rapidly detecting ten types of targeting pneumonia bacteria: Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Klebsiella pneumoniae, Pseudomonas aeruginosa, Moraxella catarrhalis, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus aureus. A set of primer pairs directed to their respective target regions contained in the DnaJ gene, etc., of the ten types of pneumonia causative bacteria is designed for the ten bacterial strains and used to amplify gene products. A set of bacterial strain-specific probe pairs is further designed for the ten bacterial strains such that the probe pairs hybridize with the amplification products via sequences in the respective target regions differing from the sequences hybridized by the set of primer pairs. A first probe-bound labeled high molecular carrier in which plural types of first probes for the pneumonia bacteria are bound to a labeled high molecular carrier and a solid-phase second probe-carrying developing support are used as the set of probe pairs to perform nucleic acid chromatography.

The invention relates to a nucleotide specific to wzt of Legionella pneumophila serogroup 1 and application thereof. The nucleotide is a nucleotide shown as SEQ ID NO: 1 and/or a nucleotide shown as SEQ ID NO: 2 and is complementary with the nucleotides. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Legionella pneumophila serogroup 1, a gene chip or a micro-array. The nucleotide specific to the wzt of the Legionella pneumophila serogroup 1 provided by the invention as well as the PCR kit, the gene chip or the micro-array including the nucleotide have the advantages of strong practical applicability, simple preparation method of the PCR kit, short detection period, high speed, strong maneuverability, easiness for industrial production, low detection cost, high accuracy and high sensitivity.

The invention provides a reagent for legionella pneumophila detection according to an in-vitro nested loop-mediated isothermal amplification method. The reagent comprises an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP, a loop primer Loop-f, a loop primer Loop-r, a nested primer FNP and a nested primer BNP. The invention further provides a novel efficient in-vitro isothermal amplification based legionella pneumophila detection method adopting the reagent. Quickness and accuracy in legionella pneumophila detection are realized by adoption of the method, sample detection can be finished in 30-45 minutes approximately, LAMP (loop-mediated isothermal amplification) reaction time is shortened by about 30%-50% as compared with that of a method with loop primers added, and great practical significance to quick disease diagnosis is achieved.

The invention discloses a kit for detecting a plurality of pathogens on the basis of a microfluidic chip and a using method of the kit. The kit comprises a multiflux microfluidic nucleic acid detection chip for actively controlling a flow passage, a preloaded dry powder detection reagent and a positive quality control material, wherein the preloaded dry powder detection reagent contains a primer and a Taqman fluorescent probe of a specific conserved sequence of mycoplasma pneumoniae, chlamydia pneumoniae and legionella pneumoniae. According to the kit, a microfluidic chip technology is adoptedand all operations after sample loading are completed by the apparatus, so that convenience for operation and high speed are achieved, detection can be completed within 30 to 60min and pollutions areavoided; a Taqman probe fluorescence PCR (polymerase chain reaction) technology is adopted for detection of the mycoplasma pneumoniae, the chlamydia pneumoniae and the legionella pneumoniae, the sequence of the primer and the probe is designed to be conserved in genes of the mycoplasma pneumoniae, the chlamydia pneumoniae and the legionella pneumoniae and high in specificity, a detection result can be obtained within 2 hours and the sensitivity can reach 10copies/mu L.

The invention provides a rapid identification method for legionella pneumophila. The rapid identification method comprises the following steps: adopting a pair of legionella pneumophila specific primers to amplify a region on a 16S rRNA (ribosomal Robonucleic Acid) gene; designing a pair of hybridization probes in an amplified segment aiming at specific sequences of the legionella pneumophila specific primers; anchoring a probe 3'-end marked FAM fluorescent gene and quenching a probe 5'-end marked BHQ1 (Black Hole Quencher 1); carrying out phosphorylation on the 3' end; analyzing the legionella pneumophila in a sample by real-time fluorescence PCR (Polymerase Chain Reaction) melting curve analysis. The detection method mainly comprises the following three steps: extracting a sample DNA (Deoxyribose Nucleic Acid), carrying out real-time fluorescence PCR and analyzing a melting curve. The method is simple and rapid to operate, is intuitive in result, is high in specificity and good in stability, and can be widely applied to rapid identification of the legionella pneumophila in clinical and environmental water samples.

The invention relates to the technical field of fluorescent PCR detection, in particular to a kit for simultaneously detecting streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis. A fluorescent PCR detection system comprises primers of specific conserved sequences of streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis and a TaqMan fluorescent probe, and the DNA sequence of the fluorescent PCR detection system is SEQ ID NO: 1-9. The kit has the advantages that multiple fluorescent quantitative PCR detection technologies are utilized, the purpose ofsimultaneously detecting three respiratory tract pathogens including streptococcus pneumoniae, legionella pneumophila and Moraxella catarrhalis is is achieved, the detection method is rapid and accurate, timeliness, specificity and sensitivity of detection are guaranteed, and the kit has important application value in the fields of disease monitoring, clinical diagnosis and the like.

The invention belongs to the field of microbial molecule detection and particularly relates to a rapid detection kit and method for a legionella pneumophila ST1 strain. A primer for rapid detection of the legionella pneumophila ST1 strain, provided by the invention, comprises a legionella specific primer, a legionella pneumophila specific primer and a legionella pneumophila ST1 strain specific primer. The primer provided by the invention has the advantages of being strong in specificity and high in accuracy; when multiple PCR is performed by utilizing four primers, whether legionella exists or not can be detected in a reaction system, and the detected legionella can be divided into legionella pneumophila or non legionella pneumophila. The detected legionella pneumophila can also be identified as a high-pathogenicity ST1 type epidemic strain. The rapid detection method has the benefits that multiple gel electrophoresis analysis is not required, the cost is reduced, the operation is simple and convenient, and detection results are accurate. The rapid detection kit and method for the legionella pneumophila ST1 strain is ideal.

The invention relates to a novel isothermal loop-mediated legionella pneumophila nucleic acid labeling detection reagent. By labeling a front inner primer in a primer group used for isothermal loop-mediated amplification with one antigen and meanwhile, labeling a rear inner primer with another antigen; a legionella pneumophila specific target gene can be labeled, while the legionella pneumophila specific target gene is amplified. After the legionella pneumophila specific target gene is labeled, an amplification product of the target gene can be rapidly detected by using matched colloidal gold test paper, so that legionella pneumophila is detected. The detection reagent disclosed by the invention is simple and rapid in operation and has strong specificity and high sensitivity.

The invention discloses a novel antibacterial plastic which comprises the following components in percentage by weight: 1-5 percent of nanometer antibacterial particles, 90-98 percent of plastic main ingredient, 1-5 percent of plasticizer, 0-2 percent of coupling agent and 0-2 percent of stabilizer, wherein the nanometer antibacterial particles are nanoscale metal particles, preferably nano zinc particles; the plastic main ingredient is polyvinyl chloride; the plasticizer is an epoxy or citrate plasticizer; the coupling agent is siloxane; and the stabilizer is a phthalate or epoxy stabilizer. The novel antibacterial plastic can generate an effect of killing five common pathogenic bacteria which cause human infections, such as multidrug resistance staphylococcus aureus (MRSA), streptococcus pneumoniae, Candida albicans, Pseudomonas aeruginosa and Aspergillus niger by 99.99 percent and has obvious functions on superbacteria, H1N1 and Legionella Pneumophila. The novel antibacterial plastic has an automatic killing mechanism, is non-toxic and non-irritant to a human body and can be used for preparing catering equipment, toys for children and other products with high antibacterial requirements. Meanwhile, the product also has the advantage of low production cost and has extremely high market promotion value and competitiveness.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and a kit for various respiratory tract pathogenic bacteria. The real-time fluorescent quantitative PCR detection kit comprises a PCR primer probe, wherein the PCR primer probe comprises a forward PCR amplification primer, a reverse PCR amplification primer and a detection probe which respectively aim at klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus, streptococcus pneumoniae, haemophilus influenzae, escherichia coli, staphylococcus hemolyticus and legionella pneumophila, wherein the sequences are respectively shown as SEQ ID NO.1-27. The kit can rapidly detect and screen nine respiratory tract pathogens at the same time, and has highsensitivity.

The invention relates to specific polymerase chain reaction (PCR) primers for detecting various serotype pathogenic bacteria of legionella pneumophilia, a method for quickly detecting by adopting a multi-PCR kit, and application. The kit comprises 10*PCR reaction liquid, MgCl2, dNTP, primers, and DNA polymerase; wherein the sequences of the primers is one or both of (1) and (2), wherein (1) wzt or wzm specific nucleotide sequences of legionella pneumophila serogroups 1, 4, 6, 10 and 13; and (2) complementary DNA sequences of DNA sequences selected from (1). The primers have high practicability for wzt specific nucleotide of common legionella pneumophila serogroups, and a PCR kit, a gene chip or a microarray comprising the nucleotide; and the PCR kit is easy and convenient to prepare, short in detection period, high in speed and accuracy, and strong in operability, detection cost is reduced, and the PCR kit is suitable for industrial production.

The invention discloses an organic solvent tolerant aminopeptidase LapA and a preparation method and application thereof, and belongs to the field of gene engineering, protein expression and purification and enzymological feature research. The aminopeptidase LapA comes from legionella pneumophila and has an amino acid sequence as shown in SEQ ID NO.1 and a nucleotide sequence as shown in SEQ ID NO.2. Serving as an exogenous protease, the aminopeptidase has favorable temperature stability and organic solvent tolerance, can adapt to extreme high-concentration organic solvent reaction conditions,and lays a favorable foundation for industrialization of the aminopeptidase LapA and application of the aminopeptidase LapA in fermented food processing industry.

The invention relates to a method of detecting pathogenic Legionella pneumophila strains by hybridizing genomic DNA of a sample suspected to contain Legionella to two or five specific sequence markers, as identified by MARKER NO. 1 through MARKER NO. 5 or homologues thereof. The invention further relates to a kit of parts comprising an array and reference materials for performing a method of the invention.