PCR primers for MLST (multilocus sequence typing) of Legionella pneumophila, typing method and application

A technology of Legionella pneumophila and typing method, which is applied in the field of PCR primers and typing for multi-locus sequence typing of Legionella pneumophila, can solve the problems of complicated operation, high economic cost, time-consuming and labor-consuming, and achieve The effect of high resolution, improved efficiency, and easy operation

Active Publication Date: 2017-12-01
南京金域医学检验所有限公司
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Problems solved by technology

However, the number of genetic loci that these two methods need to detect is relatively large, 53 and 100 respectively, and the operation is complicated, time-consuming and labor-intensive, and the economic cost is also high

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  • PCR primers for MLST (multilocus sequence typing) of Legionella pneumophila, typing method and application
  • PCR primers for MLST (multilocus sequence typing) of Legionella pneumophila, typing method and application
  • PCR primers for MLST (multilocus sequence typing) of Legionella pneumophila, typing method and application

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[0029] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of ...

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Abstract

The invention discloses PCR primers for MLST (multilocus sequence typing) of Legionella pneumophila, a typing method and an application. The PCR primers totally comprise 5 primer pairs for partial gene segments of cca, trpA, lssD, lspE and icmK, the five genetic loci cca, trpA, lssD, lspE and icmK have more single nucleotide polymorphism loci on Legionella pneumophila and have higher relative nucleotide variability, so that higher resolution can be obtained during sequence typing of the Legionella pneumophila after sequencing, and molecular epidemiological investigation and study are facilitated. When the PCR primers for MLST of the Legionella pneumophila and the typing method are applied to MLST of the Legionella pneumophila, fewer genes are needed to be detected, the sequence typing method is high in resolution and simple and convenient to operate, and the efficiency of the MLST method can be increased.

Description

technical field [0001] The invention relates to the field of microbial molecular epidemiology, in particular to a PCR primer for multi-site sequence typing of Legionella pneumophila, a typing method and an application. Background technique [0002] Legionella is an intracellular gram-negative bacillus that is ubiquitous in soil and water systems. At present, there are 59 species of Legionella, including more than 70 serotypes, and new strains are constantly isolated from the environment. In recent years, it has been found that about half of Legionella is related to human diseases, and about 90% of Legionella infections are caused by Legionella pneumophila. The life cycle of Legionella pneumophila includes two parts: the amoeba in the natural water environment (including rivers, lakes and ponds, etc.) is its main parasitic place and storage, and then it migrates from the natural water environment to The artificial water environment (including artificial fountains, air-condi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6869C12Q1/689C12Q2600/156C12Q2531/113C12Q2537/165Y02A50/30
Inventor 詹晓勇朱庆义
Owner 南京金域医学检验所有限公司
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