Kit for detecting various pathogens and use method thereof

Abstract

The invention discloses a kit for detecting mycoplasma pneumoniae, chlamydia pneumoniae and legionella pneumophila. The kit comprises a detecting tube containing a detection reagent, a positive quality control material and Rnase-free water; the detection reagent is sub-packaged in single tubes and is dry powder including primers of specific conserved sequences of the mycoplasma pneumoniae, the chlamydia pneumoniae and the legionella pneumophila and Taqman fluorescence probes; the mycoplasma pneumoniae; the chlamydia pneumoniae and the legionella pneumophila can be quickly and accurately detected, and the kit is simple and easy to use in various environments, thereby guaranteeing the timeliness, the specificity and the sensitivity of the detection; the mycoplasma pneumoniae, the chlamydia pneumoniae and the legionella pneumophila can be detected and distinguished by designing the specific primer probes for the conserved sequences of the mycoplasma pneumoniae, the chlamydia pneumoniae and the legionella pneumophila; the problems of low-temperature preservation and tedious use operation are solved, the detection reagent can be preserved at low temperature of 4 DEG C or normal temperature, and can be used for detecting on a machine as long as an extracted nucleic acid sample is added.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a kit for detecting mycoplasma pneumoniae, chlamydia pneumoniae and legionella pneumophila and a use method thereof. Background technique [0002] Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (CP) and Legionella pneumophila (Legionella pneumoniae, LP) infections are important infectious diseases in the world, which can occur in children of all ages, and respiratory tract infections are the most common , the infection site can run through the entire respiratory tract, and has certain infectivity. MP, CP, and LP are common pathogenic bacteria in children with acute upper respiratory tract infection, and they are also the main pathogenic bacteria that cause community-acquired pneumonia. Mycoplasma pneumoniae pneumonia, Chlamydia pneumoniae pneumoniae pneumonia and Legionella pneumophila pneumonia caused by the three pathogenic bacteria are atypical pneumonia, which are non-spec...

AI Technical Summary

Problems solved by technology

[0008] Immunoassay detection reagents are aimed at the antigens in the sample or the specific antibodies produced by the body caused by pathogens, and there is a window period for antibody detection, so it is easy to miss the diagnosis in the early stage of the disease; the sensitivity and specificity of the immunoassay are low, and the samples are easy to be polluted and interfering substances Many, when the concentration is too high or too low, it is easy to cause misjudgment of the results, and repeated detection and re-examination are required to confirm the diagnosis, and the reliability of the detection is poor; the detection by culture method takes a long time, the culture effect is poor, and many pathogens cannot be cultured , delaying the optimal treatment time

[0009] Although ordinary fluorescent PCR detection reagents have good sensitivity and specificity, the kits are generally composed of multiple tubes of liquid reagents, which usually need to be stored in an environment of -20°C. During operation, each tube of reagents needs to be mixed in proportion. There are high requirements for transportation and storage conditions, operation methods, etc., and it is easy to cause unreliable test results due to improper storage and operation errors

In addition, the mixed infection of Mycoplasma pneumoniae, Chlamydia pneumoniae and Legionella pneumophila is increasing day by day, but the products on the market mainly detect a single pathogen, which cannot meet the needs of rapid detection of three pathogens

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