Real-time fluorescent quantitative PCR (polymerase chain reaction) detection method and kit for various respiratory tract pathogenic bacteria

Abstract

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and a kit for various respiratory tract pathogenic bacteria. The real-time fluorescent quantitative PCR detection kit comprises a PCR primer probe, wherein the PCR primer probe comprises a forward PCR amplification primer, a reverse PCR amplification primer and a detection probe which respectively aim at klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, staphylococcus aureus, streptococcus pneumoniae, haemophilus influenzae, escherichia coli, staphylococcus hemolyticus and legionella pneumophila, wherein the sequences are respectively shown as SEQ ID NO.1-27. The kit can rapidly detect and screen nine respiratory tract pathogens at the same time, and has highsensitivity.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a real-time fluorescent quantitative PCR detection method and a kit for various respiratory pathogenic bacteria. Background technique [0002] Respiratory tract infection is a common disease worldwide and one of the leading causes of morbidity and mortality worldwide. It is mainly caused by respiratory viruses and some bacteria, mycoplasma, chlamydia, etc. It usually causes severe upper and lower respiratory tract infections in infants, the elderly and immunocompromised patients, leading to asthma, bronchiolitis, pneumonia, etc., and it is very easy to cause epidemics or outbreaks, with high morbidity and mortality. Their clinical manifestations are similar, which brings great challenges to the timely and accurate diagnosis of respiratory infectious diseases and the control of the epidemic. [0003] Respiratory tract infection is divided into upper respiratory tract ...

AI Technical Summary

Problems solved by technology

But there are following disadvantages: 1) time-consuming is long, generally need 3-5 days or longer; 2) diagnosis efficiency is low, can only each bacterium be cultivated separately; 3) false negative result is high, due to the abuse of antibiotics, bacteria in Inhibition of growth during assay results in

But there are following disadvantages: 1) prone to false positives 2) low sensitivity 3) unable to detect multivariate viruses

However, the problem of rapid detection of multiple pneumonia pathogenic bacteria at the same time cannot be solved at present.

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