Fluorescent label amplification kit for simultaneously amplifying human 27 STR loci and application of fluorescent label amplification kit

A technology of fluorescent markers and gene loci, applied in the field of molecular genetics, can solve the problems of time-consuming and inconvenient use by public security

Inactive Publication Date: 2019-12-24
百特元生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the kits on the market only contain 20 core loci, and the kits of each company are different in terms of the optimal loci. It is extremely inconvenient for the police to use it. If they want to use more loci to commit crimes The determination of suspects usually requires the combined use of multiple kits, which also takes more time

Method used

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  • Fluorescent label amplification kit for simultaneously amplifying human 27 STR loci and application of fluorescent label amplification kit
  • Fluorescent label amplification kit for simultaneously amplifying human 27 STR loci and application of fluorescent label amplification kit
  • Fluorescent label amplification kit for simultaneously amplifying human 27 STR loci and application of fluorescent label amplification kit

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Experimental program
Comparison scheme
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Embodiment 1

[0126] Embodiment 1: Application of the kit of the present invention in identification of kinship

[0127] Direct multiplex amplification was performed on two hairs from suspected father and son to detect 27 loci. The amplification method adopts the method of direct amplification: take a section of hair with hair follicles and put it directly into the amplification system so that the hair follicles are completely immersed in the liquid. The amplification procedure adopts the standard amplification procedure of the present invention, and the amplification instrument adopts ABI Proflex PCR instrument, genetic analyzer adopts 3500xl genetic analyzer, analysis software adopts GeneMapper-ID-X analysis software. The operation steps of this embodiment are as follows:

[0128] ①Use scissors to cut short the hair at 0.5cm above the hair follicle, and put the short hair with hair follicle into a 200ul PCR tube. The hair used for augmentation must have hair follicles. If there are no h...

Embodiment 2

[0136] Embodiment 2: the application of kit of the present invention in forensic medicine

[0137] The invention is mainly used in the field of forensic medicine for the construction of the DNA database of criminals and identification of the identity of criminal suspects.

[0138] The national forensic DNA database is a national DNA database of criminals established by the Ministry of Public Security of my country. Now the database has exceeded 50 million data and is the main tool for the public security system to detect cases. The database is usually built by the method of direct amplification and detection of blood samples. The identification of illegal and criminal suspects is usually complicated, and the method of amplification and detection after DNA extraction is used for detection. The invention can take into account two different inspection materials, and facilitates the construction of a national DNA database and identification of the identity of criminal suspects. T...

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Abstract

The invention provides a fluorescently label amplification kit for simultaneously amplifying human 27 STR loci. The fluorescently label amplification kit comprises 24 autosomal STR loci, one Y-chromosome STR locus, and two specific amplified primer pairs for gender-identified STR loci. The loci are D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, D2S1338, D6S1043,D22S1045, D19S433, D1S1656, D12S391, D10S1248, D2S441, vWA, D8S1179, TPOX , FGA, SE33, Penta D, DYS391, Y-INDEL and Amelogenin. The loci contain 20 core loci and four preferred loci as prescribed by the Ministry of Public Security, all sites of mainstream kits currently on the market are covered, the risk of incorrect gender identification due to the deletion of the Y-chromosome can be effectivelyprevented, and the advantages of high discrimination power and high probability of paternity exclusion are achieved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, in particular to a fluorescent marker amplification kit for simultaneously amplifying 27 human STR gene loci and its application. Background technique [0002] STR (short tandem repeats, short tandem repeats), also known as microsatellite sequence, is a short tandem repeat sequence widely present in human genome DNA, and the repeat unit is 2 to 6 nucleotides. Due to its high polymorphism and stability, and compared with AMP-FLP and VNTR genotyping methods, the amplified product length of STR genotyping method is much smaller (less than 500 bp), so the requirement for template quality is relatively high. Low, even degraded DNA templates can be analyzed. In addition, STR typing is applicable to DNA purified by various DNA purification methods, but the amount of DNA obtained by these purification methods is often not enough for Southern blot analysis. In view of the above characteristics, STR typ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/16
Inventor 冉凌飞蒿杰
Owner 百特元生物科技(北京)有限公司
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