Fluorescent label amplification kit for simultaneously amplifying human 27 STR loci and application of fluorescent label amplification kit

Abstract

The invention provides a fluorescently label amplification kit for simultaneously amplifying human 27 STR loci. The fluorescently label amplification kit comprises 24 autosomal STR loci, one Y-chromosome STR locus, and two specific amplified primer pairs for gender-identified STR loci. The loci are D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, D2S1338, D6S1043,D22S1045, D19S433, D1S1656, D12S391, D10S1248, D2S441, vWA, D8S1179, TPOX , FGA, SE33, Penta D, DYS391, Y-INDEL and Amelogenin. The loci contain 20 core loci and four preferred loci as prescribed by the Ministry of Public Security, all sites of mainstream kits currently on the market are covered, the risk of incorrect gender identification due to the deletion of the Y-chromosome can be effectivelyprevented, and the advantages of high discrimination power and high probability of paternity exclusion are achieved.

Description

technical field [0001] The invention belongs to the field of molecular genetics, in particular to a fluorescent marker amplification kit for simultaneously amplifying 27 human STR gene loci and its application. Background technique [0002] STR (short tandem repeats, short tandem repeats), also known as microsatellite sequence, is a short tandem repeat sequence widely present in human genome DNA, and the repeat unit is 2 to 6 nucleotides. Due to its high polymorphism and stability, and compared with AMP-FLP and VNTR genotyping methods, the amplified product length of STR genotyping method is much smaller (less than 500 bp), so the requirement for template quality is relatively high. Low, even degraded DNA templates can be analyzed. In addition, STR typing is applicable to DNA purified by various DNA purification methods, but the amount of DNA obtained by these purification methods is often not enough for Southern blot analysis. In view of the above characteristics, STR typ...

AI Technical Summary

Problems solved by technology

At present, most of the kits on the market only contain 20 core loci, and the kits of each company are different in terms of the optimal loci. It is extremely inconvenient for the police to use it. If they want to use more loci to commit crimes The determination of suspects usually requires the combined use of multiple kits, which also takes more time

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