Automatic sample injection system based on micro-fluidic chip

Abstract

The invention discloses an automatic sample injection system based on a micro-fluidic chip. The automatic sample injection system comprises a micro-fluidic chip and a sample injection device for inputting samples into the micro-fluidic chip, and further comprises a negative pressure control device for generating negative pressure, wherein the sample injection device comprises a porous plate for accommodating samples; a suction needle is connected to the sample injection port of the micro-fluidic chip in a sealed manner; the negative pressure control device generates air pressure from the porous plate to the micro-fluidic chip, so that the samples in the porous plate are sucked into the micro-fluidic chip through the suction needle. According to the automatic sample injection system, the samples are directly led into the micro-fluidic chip through the suction needle by virtue of the negative pressure, manual sample adding is avoided, the detection automation degree is improved, and the cross contamination risk is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an automatic sampling system based on a microfluidic chip. Background technique [0002] Microfluidics is a technology to precisely control and manipulate micro-scale fluids. This technology originated in the 1980s, and has been rapidly developed and widely used in gene chip (DNA Microarray), point-of-care (POCT) and laboratory-on-a-chip (LOC). The microfluidic chip is also called "lab on a chip". It is the main platform for the realization of microfluidic technology. It can integrate the basic operation units such as sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis. The entire analysis process is automatically completed on a micron-scale chip. Microfluidic chips have the advantages of less sample consumption, fast detection speed, easy operation, multi-functional integration, small size, and portabilit...

AI Technical Summary

Problems solved by technology

Among them, the migration speed of positive and negative ions in the electric field is inconsistent during the electrokinetic sampling process, resulting in a different concentration of the sample from the sample solution, and the change in the surface properties of the chip capillary will lead to a difference in the size of the electroosmotic flow, which greatly reduces the accuracy of the injection volume; The sample uses a syringe pump to drive the sample in the sample cell into the separation channel. In order to reduce the separation efficiency caused by the diffusion and dilution of the sample in the buffer, it is usually necessary to use multiple syringe pumps or PC-controlled solenoid valves to form a stable sample, which increases the system Cost and Difficulty of Operation

[0005] It can be seen that the microfluidic chip sampling system must rely on external pumps or different types of valve bodies to cooperate, resulting in most of the various analysis and detection instruments being in a manual and semi-automatic state.

For example, when using an ordinary microfluidic capillary electrophoresis chip for DNA fragment analysis, it is still necessary to manually add the PCR-amplified sample to the chip, which is complicated to operate, restricts the detection throughput and increases the risk of cross-contamination

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