Identification and detection method for yellow fever, Japanese encephalitis, chikungunya fever and west Nile fever, primers and probes

Abstract

The invention discloses an identification and detection method for yellow fever, Japanese encephalitis, chikungunya fever and west Nile fever, primers and probes. The identification and detection method comprises the step of rapidly, qualitatively and quantitatively detecting a yellow fever virus, a Japanese encephalitis virus, a chikungunya virus and a west Nile virus by combining polymerase chain reaction (PCR) and a liquid chip. The four pairs of primers and probes with high specificity, high sensitivity and good repeatability are provided in the method.

Description

technical field [0001] The invention belongs to the technical field of biochips and diagnostic reagents, and relates to a differential detection method for yellow fever, Japanese encephalitis, Chikungunya, and West Nile, as well as primers and probes, specifically a microsphere-based Liquid chip technology for rapid detection of multiple mosquito-borne viruses. Background technique [0002] Yellow fever (Yellow fever virus), Japanese encephalitis (first isolated from patient brain tissue in Japan, so called Japanese encephalitis virus (Japanese encephalitis virus, JEV)), West Nile fever (West Nile fever) ) and Chikungunya fever (Chikungunya fever) are important arboviral infectious diseases that threaten people's health. They are caused by yellow fever virus (YFV), Japanese encephalitis virus (JEV) ), West Nile virus (WNV) and Chikungunya virus (Chikungunya virus, CHIK). [0003] Symptoms at the initial stage of the onset are very similar and difficult to identify; and the...

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Problems solved by technology

However, there are more or less defects in these methods: for example, the isolation and culture method is technically difficult, and it usually takes more than 3 to 5 days to obtain the results of etiological diagnosis and drug susceptibility, and it is difficult to carry out etiological diagnosis and drug resistance early in clinical practice. However, it cannot meet the needs of clinical treatment of critically ill infected patients; when immunological methods are used to detect virus-specific antibodies, it is not only necessary to collect double sera in the acute phase and recovery phase of the patient's infection, but also there may be crossover between antigens and antibodies. In terms of nucleic acid detection methods, PCR, multiplex PCR, RT-PCR methods, and real-time fluorescent PCR methods based on Taqman probe technology have high sensitivity and good specificity, and are suitable for rapid detection. However, most of these methods are based on the detection of a single reaction or a small number of multiple reactions. When testing clinical samples, it takes a lot of labor to detect and exclude dozens of indicators in each sample. and cost

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