Method for extracting and detecting frisee polyphenols (levan)

Abstract

The invention belongs to the field of analysis and detection, and relates to a method for extracting and detecting frisee polyphenols (levan). An inventor adopts a GracePure<TM> SPE C18-Max column produced by GRACE Company to filter a sample in the extraction of frisee plant samples. The column can remove the polar matters from a water solution, nearly cannot absorb sugar, and can effectively reduce the detection background. A common HPLC (high performance liquid chromatography) instrument is adopted, but a PREVAIL Carbohydrate ES special sugar column produced by the GRACE Company is adopted and has good sugar separation function. The sugar column is an amino sugar column bonded by polystyrene and divinylbenzene, so the problem of disturbing other amino columns is avoided, the chemical degradation is avoided, and the stability is realized at room temperature and under the gradient elution condition; an ELSD (evaporative light-scattering detector) is used for the sugar detection, and one complete set of extraction and detection flow of the polyphenols (levan) of the frisee plant samples is firstly established.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and relates to a method for extracting and detecting Jerusalem artichoke fructan. Background technique [0002] Plant fructan is a general term for a class of water-soluble carbohydrates that are mainly composed of multiple fructosyl groups connected by glycosidic bonds. Although fructans can be found in 15% of angiosperms, the total amount of fructans in plants is generally low, only in the tubers of Jerusalem artichoke (Helianthus tuberosus, also known as devil ginger, Jerusalem artichoke); chicory (Cichoriumintybus ), burdock (Arctium lappal), and chicken feet (Dactylis glomeratus); bulbs of lilies (Liliumbrownii) and onions (Allium cepa); stems and leaves of ryegrass (Lolium perenne), barley (Hordeumvulgare), and wheat (Triticum aestivum) Relatively high content in the tissues of a few species of plants (VanLaere and Van Den Ende, 2002; Ritsema and Smeekens, 2003a). [0003] Inulin (In...

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Problems solved by technology

[0008] In traditional HPLC, although the RID detector can also be used to detect carbohydrates, the RID detector is based on the continuous measurement of the change in the refractive index of the effluent from the chromatographic column, and is only suitable for the determination of carbohydrates with weak ultraviolet (UV) absorption ; At the same time, RID is extremely sensitive to temperature, making the baseline very unstable, incompatible with gradient elution, and the detection sensitivity is very low

Therefore, the use of RID for the detection of carbohydrates is quite limited

[0009] Although the previous studies also used the HPLC-ELSD method to determine sugars in plants, there are certain defects, which are specifically reflected in (1): some studies use ordinary silica gel-based amino columns, which are not durable (due to moisture can cause self-hydrolysis of the amino stationary phase), column life is not long

At the same time, in the sample extraction, simple heating and centrifugation are performed, and non-sugar substances interfere with the detection; (2): Previous studies have mostly analyzed and detected small molecules such as monosaccharides, disaccharides, or sugar alcohols, etc. For oligosaccharides and Less research on sugars with higher degrees of polymerization

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