The invention provides a method for identifying true and false beef based on a micromolecule
fingerprint spectrum, and relates to the technical field of micromolecule
fingerprint spectrums.The method comprises the following steps of sample pretreatment and micromolecule extraction, specifically, a
beef sample to be detected is subjected to
standardization treatment, and after surface impurities are removed, the
beef sample is frozen and ground into homogenate; acquiring
mass spectrum data and constructing a spectrum; generating a three-dimensional micro-
molecular fingerprint spectrum; screening characteristic molecular markers; identification model construction and training: training the marker
data set; and performing model
verification test. According to the method, low-content adulterated meat can be stably recognized through non-
targeted screening, multi-marker collaborative judgment and combination of a
machine learning dynamic threshold value, the accuracy rate is still kept in a high-accuracy-rate state after cooking, and compared with a traditional method, the sensitivity is greatly improved; through
lipidome-
metabolome multi-
dimensional analysis, animal source adulteration and
plant source substitutes can be synchronously distinguished, a larger range of adulteration types can be covered, and the blind area of the traditional technology is solved.