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Kit for evaluating prognostic risks of breast cancer

A kit and breast cancer technology, applied in the direction of microbial determination/testing, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of high cost and price, high cost, low miRNA content, etc., and achieve relative quantitative improvement Accuracy, fast and convenient detection, and high prediction accuracy

Active Publication Date: 2015-12-30
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The content of miRNA in paraffin specimen tissue is low, which requires a very sensitive and specific detection method; the miRNA primer sequence is short, primer design is difficult, and there is no targeted software available, so primers and systems need to be optimized
At present, the high-throughput detection of miRNA generally uses chip technology, which has high cost, limited specificity and sensitivity, and cannot meet the real-time detection needs of sporadic specimens in clinical practice; the real-time detection of a small number of miRNAs mostly uses Taqman probe method, and some of them have been commercialized kits, but the cost and selling price are high

Method used

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  • Kit for evaluating prognostic risks of breast cancer
  • Kit for evaluating prognostic risks of breast cancer
  • Kit for evaluating prognostic risks of breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] An embodiment of the kit for assessing the prognostic risk of breast cancer according to the present invention, the kit includes hsa-miR-21-5p, hsa-miR-22-5p, hsa-miR-7-5p, hsa -miR-125b-5p, hsa-miR-93-5p, hsa-miR-182-5p, hsa-miR-200a-5p, hsa-miR-200b-5p, hsa-miR-200c-5p, miR-30c Amplification primers for -5p, miR-181a-5p and hsa-let-7g-5p; amplification primers for internal reference U6; reagents for extracting RNA; reagents for preparing reverse transcription reaction systems; Reagents for PCR reaction system.

[0028] The amplification primer sequence of the hsa-miR-21-5p is shown in SEQIDNo.1 and SEQIDNo.2, the amplification primer sequence of the hsa-miR-22-5p is shown in SEQIDNo.3 and SEQIDNo.4, The amplification primer sequences of the hsa-miR-7-5p are shown in SEQIDNo.5 and SEQIDNo.6, the amplification primer sequences of the hsa-miR-125b-5p are shown in SEQIDNo.7 and SEQIDNo.8, The amplification primer sequences of the hsa-miR-93-5p are shown in SEQIDNo.9 and...

Embodiment 3

[0114] Embodiment 3: the effect evaluation of kit of the present invention

[0115] figure 1 In order to adopt the kit of the present invention to jointly detect 12 miRNAs (miR-21, miR-22, miR-7, miR-125b, miR-93, miR-182, miR-200a, miR-200b, miR-200c, miR-30c , miR-181a and Let-7g) and the ROC curve of TNM staging, the results showed that compared with traditional TNM staging, the 12-miRNA-based model had better sensitivity and specificity.

[0116] The RiskScore calculation formula, 12 miRNAs correlation coefficients and cut-off values ​​of samples detected by the kit of the present invention are as follows. Among them, miR-200c, miR-7, miR-22, miR-200b, miR-200a were negatively correlated with the prognosis of breast cancer, and the remaining miRNAs were positively correlated with the prognosis of breast cancer. Calculated by the RiskScore calculation formula, individual patients whose risk value is greater than or equal to 5.44 are high-risk patients, and those less than...

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Abstract

The invention provides a kit for evaluating prognostic risks of breast cancer. Big-data clinical validation test results show that 12 miRNAs are combined to be used as a molecular marker, and the molecular marker has an outstanding advantage on the aspect of evaluation of prognostic risks of ER / PR(+)HER2(-) breast cancer. The molecular marker formed by combining the 12 miRNAs is firstly applied to development of the miRNA detection kit of ER / PR(+)HER2(-) breast cancer. The breast cancer miRNA detection kit can realize prognosis evaluation of ER / PR(+)HER2(-) breast cancer on the basis of a real-time fluorogenic quantitative PCR method, and can screen patients with high relapse risks, guide clinical individualized treatment and improve treatment accuracy.

Description

technical field [0001] The invention relates to a kit, in particular to a breast cancer stem cell-related miRNA detection kit for evaluating the prognosis risk of ER / PR(+)HER2(-) breast cancer. Background technique [0002] Breast cancer is the most common malignant tumor in women, and there are about 2 million new breast cancer patients worldwide every year. Breast cancer is a type of highly heterogeneous tumor at the molecular level. There are great differences in histomorphology, immunophenotype, biological behavior and treatment response. Patients with the same traditional pathological TNM staging have great influence on clinical treatment. Response and prognosis can vary widely. [0003] ER / PR(+)HER2(-) type is the most common molecular subtype of breast cancer, with an incidence rate of about 65% to 70% in the population. The prognosis is the best in breast cancer, and there are more patients in the early stage. Compared with other molecular subtypes of breast cance...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 宋尔卫龚畅谭维格
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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