Detection method of lincomycin residue amount in soil

A technology of lincomycin and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as accurate detection, and achieve the effect of improving accuracy and high sensitivity

Active Publication Date: 2018-04-24
NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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Problems solved by technology

[0004] The purpose of the present invention is to make up for the defects in the prior art that cannot quickly / accurately detect lincomycin in soil, there is no effective solution to the problem of matrix effect, and to provide a method for extracting lincomycin in soil and determining the residual amount method, in order to provide a method basis for the detection of lincomycin residues in soil

Method used

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  • Detection method of lincomycin residue amount in soil
  • Detection method of lincomycin residue amount in soil
  • Detection method of lincomycin residue amount in soil

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specific Embodiment approach 1

[0023] Specific embodiment one: the detection method of lincomycin residue in a kind of soil of the present embodiment, it is carried out according to the following steps:

[0024] 1. Sample preparation for the test product:

[0025] 1) Take the soil containing lincomycin, add extractant, vortex for 1 min, assisted extraction in ultrasound for 30 min, centrifuge at 3000-4000 rpm for 10 min, and take the supernatant;

[0026] 2) Evaporate the supernatant in a 30°C water bath to nearly dryness, and then redissolve it in ultrapure water to obtain the sample to be treated;

[0027] 3) Pipette methanol and ultrapure water to activate the HLB solid-phase extraction column, add the sample to be treated, pass through the column at a speed of 1mL / min, and rinse with ultrapure water after all the sample liquid flows out. After vacuuming for 5-10 minutes, the analyte was eluted with 80% methanol by volume, the eluate was collected and fixed to volume, shaken evenly, and passed through a...

specific Embodiment approach 2

[0032] Embodiment 2: This embodiment differs from Embodiment 1 in that the mass-volume ratio of lincomycin soil to extractant is 1 g: 10 mL. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0033] Embodiment 3: This embodiment differs from Embodiment 1 in that: the extractant is mixed with phosphate and acetonitrile in a ratio of 3:4 by volume. Others are the same as in the first embodiment.

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Abstract

The invention discloses a detection method of lincomycin residue amount in soil, and relates to a detection method of lincomycin residue valence in soil. The method comprises the steps of preparing reference solution, preparing test solution, adopting high performance liquid chromatography for detection, injecting standard solution and the test solution into liquid chromatograph, and calculating the lincomycin valence according to an external standard method. According to the detection method of the lincomycin residue amount in the soil, by means of optimal selection of chromatographic conditions and testing of the preparation methods of the test solution and the standard solution, a high performance liquid chromatography detection analysis method of the lincomycin in the soil is created;by means of optimization of the chromatographic conditions and purification process, the detection method of lincomycin in soil is created; methodology validation test researches indicate that the created method has high accuracy, strong specificity and good reproducibility, and can effectively detect the residual lincomycin in the soil.

Description

technical field [0001] The invention relates to the technical field of soil detection and testing, in particular to a detection method for lincomycin residual potency in soil. Background technique [0002] Veterinary antibiotics are used to prevent and treat animal diseases, improve feed utilization and promote animal growth. With the expansion of the scale of poultry farming, the use of antibiotics has also increased. Veterinary antibiotics are mainly excreted into the soil in the form of original drugs and metabolites in the form of feces and urine after passing through the animal body, which leads to the pollution of antibiotics in the soil and poses a threat to ecological security and human health. Relevant tests have found that the content of antibiotics in the soil has reached 11-300 μg / kg, mainly sulfonamides, quinolones, and tetracyclines. The establishment of trace detection and analysis methods for different types of antibiotics in soil has become a relatively po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王义张鹏金昌福李宁飞
Owner NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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