25 results about "Tissue Grafting" patented technology

The present invention provides tissue grafts having pre-fabricated attachment points (mounting points). [Solution] One or more pre-fabricated attachment points include pre-fabricated markings and / or pre-fabricated suture holes. The membrane tissue graft may be in the shape of a tube. Alternatively, the membrane tissue graft may be in the shape of a rectangle and can be used in nerve repair by wrapping around a severed or damaged nerve. The membrane tissue graft is suitable for repairing severed nerves that have a short gap of less than 5 mm between the severed ends, or nerves that do not have a gap. Therefore, a damaged or severed nerve can be repaired by implanting a membrane tissue graft onto it.

A method for preserving a tissue graft includes submerging the tissue graft in a solution containing one or more antimicrobial and / or stabilizing agents and storing the tissue graft in the solution for a period of time of at least 24 hours while the solution is in an unfrozen state. A tissue preservation system includes a solution containing one or more antimicrobial and / or stabilizing agents and a tissue graft in the solution.

Methods and devices for tissue graft fixation include fixation devices attached to an adjustable fixation loop of suture without compromising the graft or requiring additional material to complete the repair. Other fixation devices are attachable to an independent adjustable suture system. Adjustable fixation loops minimize slip / creep of the suture within the loop.

The present invention relates to a mould (1) for forming a tissue graft, preferably an autologous tissue graft for transplantation and suturing. The mould comprises a receptacle (10) and a pressure plate (30). The mould is adapted to contain layers of biological material, providing pressure to the biological material and drain out excess fluid, thus providing a fast and effective means for building a tissue graft. The receptacle (30) is adapted with a mass m, the mass adjusted to provide the specific pressure, relative to the area of a bottom surface (11) of the receptacle, wherein the tissue graft is to be manufactured. In some embodiments, the mould may further be provided with a weighted element, the weighted element adapted with a mass correlating to an area of the receptacle, thus providing a specific amount of force and thus pressure to the biological material arranged in the mould during use.

A fixture includes a first ring member and a second ring member. The first ring member has a first open center and has a first tool connector disposed at a first radial position relative to the first open center. The second ring member has a second open center coupled to the first ring member at a joint. The joint is disposed at a second radial position relative to the first open center. The joint has a second tool connector. The joint is configured to enable movement of the first ring member relative to the second ring member.

Exemplary embodiments of apparatus and method for harvesting small portions of tissue (“micrografts”) to form grafts can be provided. For example, a hollow tube can be inserted into tissue at a donor site, where a distal end of the hollow tube can have two or more points or extensions to facilitate separation of the micrografts from the surrounding tissue. The exemplary apparatus can be provided that includes a plurality of such tubes for simultaneous harvesting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, or less than about 0.3 mm, which can promote healing of the donor site and / or viability of the harvested tissue. The micrografts can be approximately cylindrical or strip-shaped, and can be placed in a biocompatible matrix to form a graft or directly into tissue at the recipient site. Such exemplary micrografts can be obtained from skin or other types of tissue, e.g., various internal organs.

Exemplary embodiments of apparatus and method for obtaining one or more portions of biological tissue (“micrografts”) to form grafts are provided. For example, a hollow tube can be inserted into tissue at a donor site, and a pin provided within the tube can facilitate controlled removal of the micrograft from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. The exemplary apparatus can include a plurality of such tubes and pins for simultaneous harvesting and / or implanting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, which can promote healing of the donor site and / or viability of the harvested tissue.

To provide tissue grafts with pre-made attachment points (points of attachment).SOLUTION: One or more pre-made attachment points may include pre-made markings and / or pre-made suture holes. The membranous tissue grafts can be in the form of a tube. The membranous tissue grafts can also be rectangular in shape and can be used in a nerve repair by wrapping the severed or damaged nerve. The membranous tissue grafts are suitable for repairing severed nerves that have a short gap or no gap with a gap of less than 5 mm between the severed stumps. Accordingly, a damaged or severed nerve can be repaired by implanting the membranous tissue grafts on to the damaged or severed nerve.SELECTED DRAWING: Figure 1

Exemplary embodiments of apparatus and method for obtaining one or more portions of biological tissue (“micrografts”) to form grafts are provided. For example, a hollow tube can be inserted into tissue at a donor site, and a pin provided within the tube can facilitate controlled removal of the micrograft from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. The exemplary apparatus can include a plurality of such tubes and pins for simultaneous harvesting and / or implanting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, which can promote healing of the donor site and / or viability of the harvested tissue.

In a method of creating a modified tissue graft, at least one exterior surface of a graft is modified by compressing, cutting and / or removing one or more portions thereof to create designed surface features which cause the tissue graft to have characteristics for a specific anatomical area. Medicants are associated with the surface features of the tissue graft, or with a second graft or layer which is associated with a base tissue graft. The tissue graft may be modified by pressing a specially configured template or die having blades thereon into the tissue graft to create a pattern of partial depth cuts.

Systems and methods include an implant device having processing circuitry configured to receive electrical signals from a free tissue graft via a plurality of electrodes attached to the free tissue graft, the free tissue graft operatively attached to a nerve of a subject. The processing circuitry is configured to generate processed signal data corresponding to the received electrical signals from each of the electrodes, and to transmit the processed signal data to the prosthesis controller. The prosthetic controller is configured to control the prosthetic device based on the processed signal data transmitted from the processing circuitry to control the prosthetic device to perform a plurality of different functions based on the processed signal data, the processed signal data generated based on the received electrical signals from each of the plurality of electrodes.

Described herein are FasL-engineered biomaterials, as well as methods of making and using such FasL-engineered biomaterials, such as for immunomodulation, such as for inducing immunosuppression and specific immune tolerance, such as for preventing or reducing the risks of rejection of cellular or tissue grafts and / or the treatment of autoimmune disorders such as Type I diabetes. In specific embodiments, the FasL-engineered biomaterials are biotinylated microgels bound to SA-FasL.

The described invention provides soft tissue grafts, hard tissue grafts, and composite soft / hard tissue grafts and methods of producing such grafts. The grafts comprise a three-dimensional carrier matrix, a growth factor composition comprising an autologous platelet-rich fibrin and a cell culture composition comprising a culture medium, a population of cells suspended in the culture medium, and cells impregnated on or in a surface of osteoconductive particles.

Methods and apparatus for tissue graft fixation include fixation devices that are attached to adjustable fixation loops of sutures without damaging the graft or requiring additional materials for repair. Other fixation devices may be attached to independent adjustable suture systems. The adjustable fixation loops minimize slippage / peristalsis of the sutures within the loop.

Methods and devices for tissue graft fixation include fixation devices attached to an adjustable fixation loop of suture without compromising the graft or requiring additional material to complete the repair. Other fixation devices are attachable to an independent adjustable suture system. Adjustable fixation loops minimize slip / creep of the suture within the loop.

An enriched amnion derived tissue graft has a stromal matrix layer and an epithelium layer. A stromal matrix layer may contain a basement membrane layer, a compact layer, a fibroblast layer, and an intermediate layer. An amnion derived tissue graft may have a plurality of tissue layers and the layers may be configured with the epithelium layers toward the inside and the stromal matrix layers toward the outside surface. An enriched portion may be coated onto or be configured within a tissue layer and have an increased concentration of cells, of proteins, growth factors, seed cells, pharmaceuticals or any combination thereof. A carrier fluid such as amniotic fluid may be used to produce an enrichment composition for coating the tissue graft. The cells within the enriched portion may be stem cells from the amnion, or cells from other tissue and may be seed cells selected based on the treatment location.

Provided herein is a method for producing a tissue graft. The graft may include a living cell and an attachment scaffold. The method may include rinsing a tissue sample; incubating the sample in a hypertonic salt solution to produce a hypertonic solution-treated tissue sample; incubating the treated tissue sample with an ionic detergent to produce a detergent-treated tissue sample; incubating the detergent-treated sample with a DNase to produce a DNase-treated scaffold; incubating the DNase-treated scaffold with a virus-inactivating compound to produce a virus- inactivated scaffold; and contacting the virus-inactivated scaffold with a living cell population to produce a tissue graft.

Systems and methods for assembling a plurality of tissue grafts are provided. A method includes applying a magnetic coating over a surface of a donor site and harvesting the plurality of micro tissue grafts from the donor site, so that an upper surface of each of the plurality of micro tissue grafts contains the coating. The method also includes arranging a magnet over the magnetic coating to induce the plurality of micro tissue grafts to organize in a desired orientation, forming a tissue construct containing the plurality of micro tissue grafts arranged in the desired orientation, and applying the tissue construct to a recipient site.

The invention provides a device for reliable support and / or transport of a tissue graft or implant, which allows for precise microscopic investigation and / or evaluation and quality control of the tissue graft or implant during handling and prior to introduction into a living being. The body of the device is a sleeve or tube housing having a rectangular shape with preferably rounded edges and two opposite openings of different size and shape. The first opening is circular in shape with a diameter suitable for connection to a tube or syringe nozzle. The second opening has an elliptical, circular, lenticular or rectangular shape, preferably with rounded edges in the case of a rectangular shape, the shape of the second opening being small enough to be inserted into a small surgical incision. The transparency of the device body and the preferably circular, rectangular shape allow for microscopic examination of the tissue graft or implant within the device and fixation of the position of the tissue graft or implant during transport and implantation. The elliptical, circular, lenticular or preferably rounded rectangular inner shape of the second opening ensures correct positioning of the tissue graft or implant during surgical implantation, thereby preventing problems such as loss of orientation of the tissue graft or implant. The invention also provides an assembly for loading, storing and / or transporting a tissue graft or implant comprising a device according to the invention. The invention also provides a washing assembly and a method for preparing a tissue graft or implant.

Disclosed herein are three-dimensional bone tissue grafts produced from stacked demineralized bone paper. Also disclosed are methods for treating a subject using tissue grafts produced from the disclosed demineralized bone paper. Also disclosed are assay systems that involves culturing bone-promoting cells on the disclosed demineralized bone paper.

Systems and methods include a muscle cuff tissue graft operatively attached circumferentially about an intact portion of a nerve of a subject; and a dermal tissue graft operatively attached to the tip of the nerve. The muscle cuff tissue graft and the dermal tissue graft are physically separated from each other along the axis of the nerve. A sensing electrode is attached to the muscle cuff graft and a stimulation electrode is attached to the dermal tissue graft. A sensing processing circuit of the implant device receives the electrical signal from the sensing electrode, generates processed motion signal data, and transmits the processed motion signal data to a prosthesis controller that controls the prosthesis device. A stimulation processing circuit of the implantation device receives the perception feedback data from the prosthesis controller, generates processed perception signal data, and transmits an electrical signal corresponding to the processed perception signal data to a stimulation electrode.

A method of preparing and / or delivering an osteochondral plug may include, positioning a tissue graft on a cutting guide, wherein the tissue graft may have been removed from a larger body of tissue to define a shaped surface. The tissue graft may include a top surface having a cartilage surface. The method may further include advancing a first cutter along a cutting direction toward the tissue graft to cut the tissue graft to form the osteochondral plug having an external wall parallel to the cutting direction and transverse to the top surface. Positioning the tissue graft on the cutting guide may include placing the shaped surface on a registration surface of the cutting guide. The registration surface may have a reference shape that corresponds to a shape of at least part of the shaped surface.

In a method of creating a modified tissue graft, at least one exterior surface of a graft is modified by compressing, cutting and / or removing one or more portions thereof, such as to create designed surface features which cause the tissue graft to have characteristics for a specific anatomical area. The modified tissue graft may include a medicated graft, such as by associating medicants with the surface features, or by associating a second graft or layer with a modified base tissue graft layer, where medicants are associated with the second graft or layer. The tissue graft may be modified by pressing a specially configured template or die, such as having blades thereon, into the tissue graft, such as to create a pattern of partial depth cuts.

Described herein is a simplified placement system and method for a tissue graft anchor by which a surgeon may introduce one or more sutures into a hole in a boney tissue, apply a precise amount of tension to the sutures to advance a soft tissue graft to a desired location, and then advance the anchor into the bone, preferably while maintaining the requisite pre-determined suture tension and without introducing spin to the suture. Particularly preferred embodiments allow for the one-handed operation, namely embodiments in which relative axial movement between the inner tensioning device and outer driver device is optionally physically constrained, for example by means of cooperating and / or compressive elements disposed in the respective hub and handle portions, are described herein. Other preferred embodiments of the present invention relate to multi-anchor constructs that may employ threaded implants exclusively, push-in implants exclusively, or a combination of threaded and push-in implants.