46 results about "Tissue Grafting" patented technology

A method of preparing and / or delivering an osteochondral plug may include, positioning a tissue graft on a cutting guide, wherein the tissue graft may have been removed from a larger body of tissue to define a shaped surface. The tissue graft may include a top surface having a cartilage surface. The method may further include advancing a first cutter along a cutting direction toward the tissue graft to cut the tissue graft to form the osteochondral plug having an external wall parallel to the cutting direction and transverse to the top surface. Positioning the tissue graft on the cutting guide may include placing the shaped surface on a registration surface of the cutting guide. The registration surface may have a reference shape that corresponds to a shape of at least part of the shaped surface.

This invention is directed to a multi-layered tissue graft comprising a collagen layer and at least one separated and washed placental tissue component and / or umbilical cord component, wherein the collagen is human collagen substantially free of non-human antigens.

A system and method for loading a suture construct on a soft tissue graft with a graft assembly. The graft assembly includes a suture construct having a closed end and an open end; a first tail of suture and a second tail of suture positioned adjacent to the open end; a first closed loop positioned at the closed end formed from the first and second tails of suture; a first splice formed by the first tail in the second tail between the closed loop and the open end; a second closed loop formed from the first and second tails of suture between the splice and the open end; and a second splice formed by the first tail in the second tail between the second closed loop and the open end.

A tape and suture construct assembly is provided. The tape and suture construct assembly is configured to be secured at its first end to a looped end of a button implant assembly, and secure to a tendon, ligament, or tissue graft at its second end by means such as suturing or whipstitching. According to an embodiment, the tape support can be made of a textile reinforcement material and comprise two limbs of suture extending therefrom. The first suture limb can be a solid limb. The second suture limb can also be solid but additionally comprise a needle portion which has a separate eyelet connected thereto.

The present invention provides tissue grafts having pre-fabricated attachment points (mounting points). [Solution] One or more pre-fabricated attachment points include pre-fabricated markings and / or pre-fabricated suture holes. The membrane tissue graft may be in the shape of a tube. Alternatively, the membrane tissue graft may be in the shape of a rectangle and can be used in nerve repair by wrapping around a severed or damaged nerve. The membrane tissue graft is suitable for repairing severed nerves that have a short gap of less than 5 mm between the severed ends, or nerves that do not have a gap. Therefore, a damaged or severed nerve can be repaired by implanting a membrane tissue graft onto it.

A method for preserving a tissue graft includes submerging the tissue graft in a solution containing one or more antimicrobial and / or stabilizing agents and storing the tissue graft in the solution for a period of time of at least 24 hours while the solution is in an unfrozen state. A tissue preservation system includes a solution containing one or more antimicrobial and / or stabilizing agents and a tissue graft in the solution.

Methods and devices for tissue graft fixation include fixation devices attached to an adjustable fixation loop of suture without compromising the graft or requiring additional material to complete the repair. Other fixation devices are attachable to an independent adjustable suture system. Adjustable fixation loops minimize slip / creep of the suture within the loop.

In some embodiments the present invention provides perfusion bioreactors and cell culture scaffolds suitable for the preparation of tissue grafts, such as bone tissue grafts. In some embodiments, the perfusion bioreactors comprise a graft chamber and / or a graft chamber insert configured to hold a tissue graft having a certain shape and / or certain dimensions, and / or to allow culture of such tissue grafts under press-fit direct perfusion conditions. In some embodiments, the perfusion bioreactors comprise an equilibration chamber.

The present invention relates to a mould (1) for forming a tissue graft, preferably an autologous tissue graft for transplantation and suturing. The mould comprises a receptacle (10) and a pressure plate (30). The mould is adapted to contain layers of biological material, providing pressure to the biological material and drain out excess fluid, thus providing a fast and effective means for building a tissue graft. The receptacle (30) is adapted with a mass m, the mass adjusted to provide the specific pressure, relative to the area of a bottom surface (11) of the receptacle, wherein the tissue graft is to be manufactured. In some embodiments, the mould may further be provided with a weighted element, the weighted element adapted with a mass correlating to an area of the receptacle, thus providing a specific amount of force and thus pressure to the biological material arranged in the mould during use.

A fixture includes a first ring member and a second ring member. The first ring member has a first open center and has a first tool connector disposed at a first radial position relative to the first open center. The second ring member has a second open center coupled to the first ring member at a joint. The joint is disposed at a second radial position relative to the first open center. The joint has a second tool connector. The joint is configured to enable movement of the first ring member relative to the second ring member.

Exemplary embodiments of apparatus and method for harvesting small portions of tissue (“micrografts”) to form grafts can be provided. For example, a hollow tube can be inserted into tissue at a donor site, where a distal end of the hollow tube can have two or more points or extensions to facilitate separation of the micrografts from the surrounding tissue. The exemplary apparatus can be provided that includes a plurality of such tubes for simultaneous harvesting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, or less than about 0.3 mm, which can promote healing of the donor site and / or viability of the harvested tissue. The micrografts can be approximately cylindrical or strip-shaped, and can be placed in a biocompatible matrix to form a graft or directly into tissue at the recipient site. Such exemplary micrografts can be obtained from skin or other types of tissue, e.g., various internal organs.

Exemplary embodiments of apparatus and method for obtaining one or more portions of biological tissue (“micrografts”) to form grafts are provided. For example, a hollow tube can be inserted into tissue at a donor site, and a pin provided within the tube can facilitate controlled removal of the micrograft from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. The exemplary apparatus can include a plurality of such tubes and pins for simultaneous harvesting and / or implanting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, which can promote healing of the donor site and / or viability of the harvested tissue.

The utility model discloses a soft tissue graft fixator and a tendon weaving system base, and relates to the technical field of medical instruments. The soft tissue graft fixator comprises a main body piece, an auxiliary body piece, a connector and a fastening piece. The at least one connector is connected to the main body piece, and the connector is configured to be detachably connected with a soft tissue clamp or a hook column; the main body piece is provided with a main body clamp face, and the auxiliary body piece is provided with an auxiliary body clamp face. The fastening piece penetrates through the main body piece and is in threaded connection with the auxiliary body piece. The fastening piece is configured to be capable of rotating so as to drive the auxiliary body piece to be close to or away from the main body piece, and then the auxiliary body clamp face is close to or away from the main body clamp face. The tendon braiding system base includes a soft tissue graft fixture. The utility model aims to provide the soft tissue graft fixator and the tendon weaving system base, the functions of the soft tissue graft fixator and the tendon weaving system base meet the surgical requirements, the size is small, the structure is relatively simple, the manufacturing cost and the selling price can be effectively reduced, the soft tissue graft fixator and the tendon weaving system base can be placed together during cleaning and sterilization, and the sterilization cost can be effectively reduced.

The present invention relates to a composition for fat grafting containing adipose tissue treated with nervonic acid. According to the present invention, during fat tissue graft surgery, the survival rate and engraftment rate of fat included in grafted adipose tissue may be increased, thereby improving efficiency of fat tissue grafting.

To provide tissue grafts with pre-made attachment points (points of attachment).SOLUTION: One or more pre-made attachment points may include pre-made markings and / or pre-made suture holes. The membranous tissue grafts can be in the form of a tube. The membranous tissue grafts can also be rectangular in shape and can be used in a nerve repair by wrapping the severed or damaged nerve. The membranous tissue grafts are suitable for repairing severed nerves that have a short gap or no gap with a gap of less than 5 mm between the severed stumps. Accordingly, a damaged or severed nerve can be repaired by implanting the membranous tissue grafts on to the damaged or severed nerve.SELECTED DRAWING: Figure 1

Exemplary embodiments of apparatus and method for obtaining one or more portions of biological tissue (“micrografts”) to form grafts are provided. For example, a hollow tube can be inserted into tissue at a donor site, and a pin provided within the tube can facilitate controlled removal of the micrograft from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. The exemplary apparatus can include a plurality of such tubes and pins for simultaneous harvesting and / or implanting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, which can promote healing of the donor site and / or viability of the harvested tissue.

An enriched amnion derived tissue graft has a stromal matrix layer and an epithelium layer. A stromal matrix layer may contain a basement membrane layer, a compact layer, a fibroblast layer, and an intermediate layer. An amnion derived tissue graft may have a plurality of tissue layers and the layers may be configured with the epithelium layers toward the inside and the stromal matrix layers toward the outside surface. An enriched portion may be coated onto or be configured within a tissue layer and have an increased concentration of cells, of proteins, growth factors, seed cells, pharmaceuticals or any combination thereof. A carrier fluid such as amniotic fluid may be used to produce an enrichment composition for coating the tissue graft. The cells within the enriched portion may be stem cells from the amnion, or cells from other tissue and may be seed cells selected based on the treatment location.

In a method of creating a modified tissue graft, at least one exterior surface of a graft is modified by compressing, cutting and / or removing one or more portions thereof to create designed surface features which cause the tissue graft to have characteristics for a specific anatomical area. Medicants are associated with the surface features of the tissue graft, or with a second graft or layer which is associated with a base tissue graft. The tissue graft may be modified by pressing a specially configured template or die having blades thereon into the tissue graft to create a pattern of partial depth cuts.

System and methods include an implant device with processing circuitry configured to receive electrical signals from a free tissue graft surgically attached to a nerve of a subject through multiple electrodes attached to the free tissue graft. The processing circuitry is configured to generate processed signal data corresponding to electrical signals received from each of the electrodes, and to transmit the processed signal data to a prosthetic controller. The prosthetic controller is configured to control a prosthetic device, based on the processed signal data transmitted from the processing circuitry, to perform different functions based on processed signal data generated based on electrical signals received from each of the plurality of electrodes.

Devices and systems are provided for associating secondary materials with tissue grafts, both ex-situ and in-situ. The secondary materials may be medicants, an external cellular matrix factor, a cell, an enzyme, or an element or compound, such as oxygen. In a method, different secondary materials are delivered to a tissue graft via a delivery device at different times, such as at different stages of a healing process.

Devices and systems are provided for associating secondary materials with tissue grafts, both ex-situ and in-situ. The secondary materials may be medicants, an external cellular matrix factor, a cell, an enzyme, or an element or compound, such as oxygen. In a method, different secondary materials are delivered to a tissue graft via a delivery device at different times, such as at different stages of a healing process. The delivery device may be associated with a wound cover or bandage.

Systems and methods include an implant device having processing circuitry configured to receive electrical signals from a free tissue graft via a plurality of electrodes attached to the free tissue graft, the free tissue graft operatively attached to a nerve of a subject. The processing circuitry is configured to generate processed signal data corresponding to the received electrical signals from each of the electrodes, and to transmit the processed signal data to the prosthesis controller. The prosthetic controller is configured to control the prosthetic device based on the processed signal data transmitted from the processing circuitry to control the prosthetic device to perform a plurality of different functions based on the processed signal data, the processed signal data generated based on the received electrical signals from each of the plurality of electrodes.

To provide an apparatus and method for obtaining one or more portions of biological tissue (micrografts) to form grafts.SOLUTION: A hollow tube 1010 can be inserted into tissue at a donor site, and a pin 1040 provided within the tube can facilitate controlled removal of micrografts from the tube. Micrografts can be harvested and directly implanted into an overlying biocompatible matrix through coordinated motion of the tube and pin. A needle can be provided around the tube to facilitate a direct implantation of a micrograft into a remote recipient site or matrix. An exemplary apparatus 1100 can include a plurality of such tubes and pins for simultaneously harvesting and / or implanting a plurality of micrografts. The harvested micrografts can have a small diameter, e.g., less than about 1 mm, which can promote healing of the donor site and / or viability of the harvested tissue.SELECTED DRAWING: Figure 11

Described herein are FasL-engineered biomaterials, as well as methods of making and using such FasL-engineered biomaterials, such as for immunomodulation, such as for inducing immunosuppression and specific immune tolerance, such as for preventing or reducing the risks of rejection of cellular or tissue grafts and / or the treatment of autoimmune disorders such as Type I diabetes. In specific embodiments, the FasL-engineered biomaterials are biotinylated microgels bound to SA-FasL.

The present disclosure provides methods and systems for receiving, with processing circuitry of an implant device, electrical signals from free tissue grafts attached to nerves through bipolar electrode pairs, each having a positive and a negative electrode, in electrical communication with the free tissue grafts. The free tissue grafts are surgically attached to a subject such that the free tissue grafts are entirely surrounded by and in direct contact with non-grafted tissue of the subject, the free tissue grafts being autografts of tissue that is harvested from the subject, devascularized, and deinnervated prior to being surgically attached to the subject. The electrical signals from the free tissue graft have a voltage amplitude of greater than or equal to about 150 microvolts. The processing circuitry processes and wirelessly transmits the electrical signals to a prosthetic controller that controls a prosthetic device.

The described invention provides soft tissue grafts, hard tissue grafts, and composite soft / hard tissue grafts and methods of producing such grafts. The grafts comprise a three-dimensional carrier matrix, a growth factor composition comprising an autologous platelet-rich fibrin and a cell culture composition comprising a culture medium, a population of cells suspended in the culture medium, and cells impregnated on or in a surface of osteoconductive particles.

Methods and apparatus for tissue graft fixation include fixation devices that are attached to adjustable fixation loops of sutures without damaging the graft or requiring additional materials for repair. Other fixation devices may be attached to independent adjustable suture systems. The adjustable fixation loops minimize slippage / peristalsis of the sutures within the loop.

Methods and devices for tissue graft fixation include fixation devices attached to an adjustable fixation loop of suture without compromising the graft or requiring additional material to complete the repair. Other fixation devices are attachable to an independent adjustable suture system. Adjustable fixation loops minimize slip / creep of the suture within the loop.

Exemplary embodiments of an apparatus for harvesting small portions of tissue ("micrografts") to form grafts can be provided. For example, a hollow tube can be inserted into tissue at a donor site, where a distal end of the hollow tube can have two or more points or extensions to facilitate separation of the micrografts from the surrounding tissue. The exemplary apparatus can be provided that includes a plurality of such tubes for simultaneous harvesting of a plurality of micrografts. The harvested micrografts can have a small dimension, e.g., less than about 1 mm, or less than about 0.3 mm, which can promote healing of the donor site and / or viability of the harvested tissue. The micrografts can be approximately cylindrical or strip-shaped, and can be placed in a biocompatible matrix to form a graft or directly into tissue at the recipient site. Such exemplary micrografts can be obtained from skin or other types of tissue, e.g., various internal organs.

This invention concerns a method for producing a tissue construct comprising microtissues suspended or resuspended in a volume of a blood sample, such as a whole blood sample or a processed blood sample, and exposing the suspension to a static acoustic wave field causing aggregates of microtissues to arrange in patterns defined by the waves. The tissue constructs are layers of coagulated blood containing the patterned microtissues which may be used for a variety of purposes, including for tissue grafting or for diagnostic purposes. The constructs may also be layered to compose more complex tissue structures.