121 results about "Amniotic Sheet" patented technology

The present invention relates to an incubation system for liquid-based incubation of prematurely born infants, comprising: —an inner chamber forming an amniotic basin comprising amniotic fluid, said basin being configured for holding said infant and being made from a flexible material configured for expanding said inner chamber volume in correspondence with the growth of said infant; —an outer chamber enclosing said inner chamber and comprising a temperature regulation fluid, —a fetal connection port, arranged for connecting with the umbilical cord of said infant, said umbilical cord providing a port in said inner chamber to said infant for providing dialyzation and nutrition compounds to said infant via said umbilical cord; —a fetal control unit, connected to said fetal connection port for control of said dialyzation and control of said provided nutrition by monitoring and controlling one or more of a pressure, flow and temperature thereof; an amniotic fluid circulation unit, arranged for connecting with an inlet / outlet port of said inner chamber and comprising a pump for circulating said amniotic fluid from said inner chamber and through a purification system located outside said inner chamber; and —a temperature regulation fluid control unit, arranged for connecting with an inlet / outlet port of said outer chamber and comprising a pump for circulating said temperature regulation fluid from said outer chamber and through a heat exchanger system located outside said inner chamber.

The invention discloses application of amniotic mesenchymal stem cells in preparation of a medicine for improving and / or treating premature ovarian failure. The invention finds that the amniotic mesenchymal stem cells can significantly improve the granular cell proliferation ability and hormone secretion ability, and the premature ovarian failure animal model proves that the amniotic mesenchymal stem cells have leading treatment effects in the aspects of restoring the ovarian functions (hormone secretion, ovary volume and follicle number of each level) of POF mice and improving fertility again. And a foundation is laid for optimizing a treatment strategy of premature ovarian failure and promoting clinical application of mesenchymal stem cell treatment.

The invention discloses a decellularized amniotic membrane matrix composite scaffold, a preparation method thereof and application of the decellularized amniotic membrane matrix composite scaffold in orbital bone defect repair, and relates to the technical field of biomedical engineering and biomedical materials. In order to solve the limitation of an existing orbit implant material, methacrylic anhydride (MA) is grafted to HAM, methacrylate HAMMA hydrogel is synthesized, and then the decellularized amnion matrix composite scaffold (HAMMA / HAP / BMP-2) is prepared by combining a simulated body fluid biomimetic mineralization method with 3D printing and ultraviolet curing. The composite scaffold material prepared by the invention can regulate the release of BMP-2 and maintain the effective drug concentration of a bone defect area so as to regulate the immune microenvironment of an early bone defect repair area, collect osteoblasts and accelerate the repair of bone defects.

The invention relates to the technical field of tissue repair, in particular to a placenta amnion decellularized material as well as a preparation method and application thereof. The amnion is subjected to decellularization treatment by adopting a high-salt freeze-thaw combined composite enzymolysis method, and the result proves that the amnion decellularization material prepared by the invention is a high-activity bioremediation material, and the natural three-dimensional structure and bioactive components of an amnion extracellular matrix are reserved to the greatest extent; and the prepared filling material has better biocompatibility and tissue inductivity.

The invention discloses an amniotic epithelial stem cell screening method and device based on microscopic image feature extraction, relates to the technical field of microscopic image analysis and cell screening, and is used for solving the problem that group topology and internal activity in amniotic epithelial stem cell lossless screening are difficult to collaboratively quantify. The method comprises the following steps: firstly, performing enhancement processing on a phase difference microscopic image, and extracting a kernel centroid and a global gradient energy diagram; then, constructing a Voronoi jurisdiction, and coupling a cytoplasm texture entropy and a gray scale attenuation gradient to generate a cytoplasm compactness factor; meanwhile, calculating a cell gap topology consistency index based on mapping of the Voronoi boundary and the gradient map; and finally, weighting and correcting the compactness factor by using a topological index to obtain a dryness maintenance confidence coefficient, and outputting a target coordinate. Through dual verification of geometric topology and physical gradient, combined constraint screening of internal and external characteristics of cells is realized.

The invention relates to the technical field of tissue repair, in particular to an amnion decellularized matrix for a filling material and a preparation method of the amnion decellularized matrix. The amnion decellularized matrix with the tissue repair function is separated and prepared from amnion tissue, specific processes such as enzymolysis are adopted in the preparation method of the amnion decellularized matrix, and the content of growth factors, collagen, glycosaminoglycans and other components in the amnion decellularized matrix can be effectively increased; the material can meet the repairing function of different types of tissues, is suitable for being used as a personalized biological filling material, and has a wide application prospect.

A multi-part processed human amniotic composition configured to treat Peyronie's disease in a subject in need thereof by intracorporeal injection of the composition into the corpus cavernosum of the subject to reduce plaque size associated with Peyronie's disease. The multi-part processed human amniotic composition includes a micronized human amnion composition; and an aqueous human amniotic fluid filtrate configured to reconstitute and suspend the micronized human amnion composition therein. In certain aspects, the multi-part processed human amniotic compositions are not processed with exogenous enzymes during production thereof and do not include exogenous enzymes, such as collagenase, added thereto.

The present invention belongs to the technical field of image processing, and discloses a three-dimensional face imaging method, device, equipment and storage medium. The method includes: acquiring original three-dimensional volume data of a tissue to be measured; determining the face position and amniotic membrane position of the tissue to be measured according to the original three-dimensional volume data and a preset threshold; determining a target positioning point profile according to the face position and the amniotic membrane position; performing transparency clearing on the original three-dimensional volume data according to the target positioning point profile to obtain three-dimensional face data of the tissue to be measured; and completing three-dimensional face imaging display of the tissue to be measured according to the three-dimensional face data. By the above method, a target positioning point profile is determined based on the amniotic membrane position and the face position, so as to identify the tissue information before the face of the tissue to be measured. Finally, transparency clearing is performed on the original three-dimensional volume data according to the target positioning point profile to complete three-dimensional face imaging display of the tissue to be measured, thereby automatically obtaining a clear, comprehensive and complete face image of the object to be measured, and improving the image rendering effect.

The present invention relates to an amnionoscope and a fitting method thereof, wherein the amnionoscope comprises an amnion area, a transition area and a landing area; the transition area is connected to the landing area, and in a top-down perspective, the landing area surrounds the transition area; a connection path is provided on the top of the transition area around its circumference, and the amnion area is installed on the transition area through the connection path; the connection path comprises a plurality of fluid channels and a plurality of connection components; the plurality of fluid channels are cross-arranged to form a grid-shaped path body, and the plurality of connection components are located one by one in a plurality of grid areas of the path body; the fluid channel is filled with a biocompatible adhesive, the transition area is connected to the amnion area through the biocompatible adhesive, and the amnion area abuts against the plurality of connection components, and the plurality of connection components form physical adsorption on the amnion area, and the beneficial effect is that the transition area forms a main connection and an auxiliary connection with the amnion area through the connection channel, and a stable connection between the amnion and the transition area is achieved without affecting the biological characteristics of the amnion, thereby ensuring the subsequent use effect of the amnionoscope.

An amniotic membrane insert configured for self-retaining, sutureless, clear-cornea application to an ocular surface. The insert has an outer edge and an inner edge inside the outer edge. The inner edge defines an opening sized to extend around a circumference of a cornea. The outer edge of the insert has a shape that is non-circular in which a height of the insert is greater than a width of the insert. Related devices, systems, and methods are provided.

The utility model belongs to the technical field of membrane rupturing devices, and particularly relates to an amniotic membrane rupturing device for gynaecology and obstetrics, which comprises a handle, one end of the handle is fixedly connected with a membrane rupturing hook, and the membrane rupturing hook is arranged towards the other end of the handle; the front face and the back face of the handle are each provided with a liquid guide groove formed in the length direction of the handle, the handle is provided with penetrating holes corresponding to the liquid guide grooves, and the penetrating holes are all formed in the ends, away from the membrane breaking hooks, of the liquid guide grooves. A handheld part is arranged at one end, far away from the membrane rupture hook, of the handle, and an annular groove is further formed in the handle and is formed between the handheld part and the liquid guide groove; the utility model provides the amniotic membrane rupture device for the obstetrics and gynecology department, which can be used for guiding amniotic fluid.

The invention provides an amniotic membrane mirror with low foreign body sensation and a preparation method thereof.The amniotic membrane mirror is composed of a large ring, a small ring and a biological amniotic membrane, the inner side of the large ring is provided with a weft groove and a warp groove, the biological amniotic membrane is located between the large ring and the small ring, the small ring is embedded into the weft groove of the large ring to clamp and fix the biological amniotic membrane to the inner side of the large ring, and the small ring is embedded into the warp groove of the large ring to clamp and fix the biological amniotic membrane to the inner side of the large ring. The large ring is made of soft materials, the small ring is made of hard materials, after the biological amniotic membrane is clamped and fixed through the large ring and the small ring, a patient wears the biological amniotic membrane in an amniotic membrane mirror mode, the large ring with the largest contact area with the ocular surface is made of the soft materials, foreign body sensation after wearing can be greatly reduced, and the biological amniotic membrane has wide application prospects and is beneficial to application and popularization.

The application provides an amniotic membrane stem cell and a separation method and a hydrogel 3D culture method thereof, relates to the technical field of cell culture, and comprises the following steps: placing amniotic membrane fragments in a pre-digestion buffer in sequence and incubating for 10-20 min, placing the amniotic membrane fragments in a mixed enzyme solution and performing digestion treatment, filtering, and obtaining a filtrate and residual amniotic membrane fragments; centrifuging the filtrate to obtain a cell precipitate, and performing differential adhesion culture to obtain amniotic membrane epithelial stem cells; adding a culture medium to resuspend the residual amniotic membrane fragments so that the residual amniotic membrane fragments are in contact with a culture container and are in a non-suspended state, performing adhesion culture, and obtaining amniotic membrane mesenchymal stem cells that have climbed out. Compared with the number of cells and the cell survival rate of cells that are subjected to digestion treatment of other mixed enzymes, the number of cells obtained by digestion treatment of the mixed enzyme is significantly increased, and the cell survival rate is higher; compared with a step-by-step digestion strategy, the mixed enzyme digestion one-step method is more simple, and efficient and synchronous separation is achieved. The technical problem of a significant decrease in cell activity caused by the step-by-step digestion separation strategy in the prior art is solved.

The invention belongs to the technical field of biological medicines, and particularly relates to amniotic mesenchymal stem cells for treating gonitis as well as a preparation method and application of the amniotic mesenchymal stem cells. The preparation method of the amniotic mesenchymal stem cells comprises the following specific steps: (1) separating to obtain amniotic tissues; (2) digesting, filtering and centrifuging the amniotic membrane tissues to obtain cell precipitates; (3) precipitating and resuspending the cells, inoculating the cells to a culture medium for culture, digesting, centrifuging, and discarding supernatant to obtain P0-generation amniotic mesenchymal stem cells; and (4) inoculating the P0-generation amniotic mesenchymal stem cells into a culture medium for subculture to obtain the amniotic mesenchymal stem cells. By adding teprenone and schaftoside into the basic culture medium, the cell dryness of the amniotic mesenchymal stem cells can be maintained, and the osteogenic differentiation capability of the amniotic mesenchymal stem cells is improved, so that the gonitis is effectively treated. Meanwhile, fetal calf serum is not added into the basic culture medium, so that the safety of clinical application is improved.

The present invention provides an amniotic membrane-loaded contact lens, its preparation method, and its application. This invention innovates the bonding method between amniotic membrane and contact lens, replacing the previous method of simply bonding the amniotic membrane and contact lens with medical biological glue. This simplifies the bonding process between amniotic membrane and contact lens, making it suitable for industrial application. It also addresses the issues of traditional sutured amniotic membranes, which suffer from decreased mechanical support, rupture, and uneven coverage over time as they cover the ocular surface. The optimization and selection of materials not only meet the basic requirements of a non-toxic and harmless adhesive, but also take into account biocompatibility and the cornea's requirements for contact lens oxygen permeability. The use of amniotic membranes is more comfortable, eliminating the need for complex surgical procedures and the irritation associated with sutures. The design of using a contact lens as an amniotic membrane support allows amniotic lenses to provide superior support and protection for the covered ocular surface.

The invention relates to the technical field of medical tools, in particular to an artificial membrane rupturing structure which comprises a barrel and a suction cup mounted at the top end of the barrel and further comprises a guide seat, a membrane rupturing device, a membrane rupturing device and a membrane rupturing device, the guide seat is mounted in the barrel, and a through hole is formed in the guide seat; the adsorption mechanism is installed in the cylinder body and connected with the guide seat, and the adsorption mechanism enables the suction cup to be adsorbed to the surface of the amniotic membrane; the artificial membrane rupturing structure is different from the prior art, in the using process of medical staff, the amniotic membrane can be clamped and fixed only by attaching the suction cup to the amniotic membrane and then pulling the hollow push rod, then puncturing is automatically conducted, the flow speed after membrane rupturing is stable, the membrane rupturing can be easily completed even though the medical staff with less experience, and operation is easy; the amniotic fluid after rupture of the membrane automatically flows to the outside through the drainage tube from the inside of the cylinder, and the amniotic fluid is not in contact with the uterus of the pregnant woman, so that the probability of infection in the uterus of the pregnant woman is greatly reduced, and the turbidity of the amniotic fluid flowing out can be directly observed.

The invention relates to the technical field of obstetrical nursing, in particular to an obstetrical nursing membrane rupture midwifery device with a negative pressure balancing function, which comprises an inserting cylinder, an endoscope is fixedly mounted at the upper part of the rear end in the inserting cylinder, and a membrane rupture assembly is arranged at the rear end in the inserting cylinder. The film breaking assembly comprises a partition plate fixed to the rear end of the interior of the inserting barrel, a plurality of film guiding holes are formed in the partition plate, a cutter groove is formed in the rear end face of the partition plate, and a film breaking blade is movably installed in the cutter groove. According to the amniotic membrane rupturing device, a baffle can block a fetus in an amniotic membrane, so that the soft outer wall of the amniotic membrane enters a membrane guiding hole, the fetus in the amniotic membrane cannot be damaged during rupture of the amniotic membrane, and meanwhile, a membrane rupturing blade is located in an inserting cylinder, so that when rupture of the amniotic membrane is carried out, the fetus can be ruptured conveniently. And the membrane rupture blade does not hurt a fetus or a puerpera.

The invention relates to the technical field of medical instruments, in particular to a frameless amniotic membrane mirror device, a manufacturing method and a using method, and the frameless amniotic membrane mirror device comprises an amniotic membrane tissue layer and a biocompatible thin film layer formed by spraying a mixed solution of polymethyl acrylate and polyvinyl alcohol; the overall diameter of the device is 14 mm, the rise of the device is 6.2 + / -0.1 mm, and the device can be directly implanted into the ocular surface to cover the cornea without a frame support. The manufacturing method comprises the steps of amniotic membrane treatment, model eye forming, mixed solution preparation, membrane spraying and forming, trimming and sterilization. The application method relates to implantation after surface anesthesia, fitting observation and subsequent taking-out. Through the frameless conformal fitting design, the problems that an existing framed amniotic membrane mirror is displaced and cannot be worn by a patient with strong foreign body sensation and shallow conjunctival sac are solved, and meanwhile secondary trauma of a suture operation is avoided. And the residual film after the amniotic membrane is dissolved can continuously provide protection, and the amniotic membrane has the advantages of good fitness, high patient comfort, simplicity and convenience in operation and good safety.

This disclosure generally relates to grafts having exudate control for treating a wound of a subject and methods thereof. In some embodiments, the grafts comprising a first layer comprising a first portion and a second portion. In some embodiments, the first portion comprises amnion, chorion, or a combination thereof. In some embodiments, the second portion comprises a swellable hydrogel. In other embodiments, the grafts comprise a first layer comprising amnion, chorion, or a combination thereof and a second layer, disposed on the first layer, comprising the swellable hydrogel. Additionally, in some embodiments, the grafts may further comprise one or more additional layers, for example, a third layer, adjacent the second layer, comprising an adsorbent material; and / or an adhesive fourth layer adjacent the third layer for adhering said graft to the skin of a subject. Other embodiments are directed toward methods of bonding two or more layers within the graft.

The invention relates to an in-utero ventriculoamniotic shunting device that includes a composite shunt tube composed of polymer material, e.g., silicone-based material, and metallic wire, having a bend or curve formed in the length of the shunt tube, with one or more anchors composed of super-elastic wire or mesh, e.g., shape memory alloy wire or mesh structures, attached to the shunt tube, and a one-way passive valve composed of a thin polymer membrane. The anchors are effective to prevent migration and dislodgement of the shunting device following its deployment, and the valve is effective to prevent the backflow of amniotic fluid.

The application relates to the field of medical devices, in particular to a support frame for cervical cerclage surgery, which comprises a transport pipe, one end of the transport pipe being provided with a pipe cover used for buckling to close one end of the transport pipe; and a filling bag, which is made of flexible material and the inside of which is communicated with the other end of the transport pipe. The support frame for cervical cerclage surgery can solve the problem that during the cervical cerclage surgery, the amnion bag slides to the vagina through the cervix, thereby pushing against the cervical orifice to make the cervical orifice keep excessively open, which makes it difficult for a doctor to perform a suture operation on the cervical orifice and to pull up the cervical orifice, the support frame is convenient for the doctor to perform the suture operation on the cervical orifice and to pull up the cervical orifice, and the support frame is easy to be collected.

The utility model provides a replaceable amniotic membrane cutting device. The replaceable amniotic membrane cutting device comprises a device body, a tool bit and a base. The device body comprises an upper cutter body and a lower cutter body, the upper cutter body and the lower cutter body are connected through a connecting piece, a groove is formed in the tail end of the upper cutter body, a round hole is formed in the lower portion of the tail end of the upper cutter body, and a first threaded hole is formed in the side face of the tail end of the upper cutter body. The head end of the tool bit is sharp, the tail end of the tool bit is thick, and the tail end of the tool bit is mounted in the groove; the base is located at the tail end of the lower knife body, and an embedding groove is formed in the base; the scalpel head is designed to be fixed in a clamping mode, the upper scalpel body is divided into a plurality of modules, the scalpel head is convenient to disassemble and replace, the diameter of the scalpel head can be changed according to operation requirements, and therefore the functions of high precision, high efficiency, easy operation and the like are achieved.

The application relates to the technical field of biomaterials, in particular to a crosslinked amniotic membrane acellular matrix filling material and a preparation method thereof. The crosslinked amniotic membrane acellular matrix filling material is prepared by using a photo-crosslinking technology, and the specific preparation method is as follows: placing an acellular amniotic membrane matrix in a pretreatment agent phosphate buffer solution to perform pretreatment, then performing functionalization treatment on the acellular amniotic membrane extracellular matrix, preparing a photo-crosslinking solution, immersing the treated acellular amniotic membrane extracellular matrix in the photo-crosslinking solution, permeating at room temperature, and finally performing irradiation under a UV light source to form a stable three-dimensional network structure. The material has high mechanical strength, controllable degradability and excellent biocompatibility, and is suitable for tissue repair and regenerative medicine.

Provided herein is a decellularized amnion-based article and uses thereof. In particular, provided herein is a decellularized amnion patch and uses thereof, for example, in tissue regeneration and repair.

The invention discloses an efficient production process of an umbilical cord mesenchymal stem cell preparation in the field of stem cells, which comprises the following steps: S101, collection and separation of umbilical cord mesenchymal stem cells: under a sterile condition, separating Walton's jelly by adopting a tissue block culture method, taking out an umbilical cord from an umbilical cord bottle, transferring the umbilical cord into a sterile culture dish, and reserving a sample of a protective solution in the umbilical cord bottle for sterile detection; washing the umbilical cord with normal saline, cutting off two ends of the umbilical cord, discarding the umbilical cord, segmenting the umbilical cord for about 3-4cm, washing to remove blood, umbilical cord veins, two arteries and amniotic membranes, collecting the Walton's jelly, and transferring the Walton's jelly into a 50ml centrifugal tube; adding a small amount of the culture medium, cutting the Walton's gum into pieces with a size of about 1mm < 3 > by using scissors, and centrifugally washing for two times by using normal saline; the preparation produced by the invention is negative in detection of hepatitis B virus, hepatitis C virus, human immunodeficiency virus, syphilis antibody, cytomegalovirus and mycoplasma, and is high in extraction efficiency and convenient to popularize.

The invention provides amniotic membrane preserving fluid and application thereof. The amniotic membrane preserving fluid is prepared from the following components: sodium chloride injection, glycerol, trehalose and sodium hyaluronate, the volume ratio of the sodium chloride injection to the glycerol is (8-24): (8-18); the volume mass ratio of the sodium chloride injection to the trehalose is (8-24): (0.5-6); the mass ratio of the trehalose to the sodium hyaluronate is (0.5-6): (0.006-0.15). Compared with an existing amniotic membrane preservation method, the amniotic membrane preservation liquid can be stored at a high temperature, so that the risk that the amniotic membrane is frostbitten can be effectively reduced, even if the amniotic membrane is stored at the high temperature, the amniotic membrane can have high biological activity and multidirectional differentiation potential, and various natural active ingredients of the amniotic membrane can be effectively reserved. Meanwhile, the amniotic membrane preserving fluid is simple in component, has good compatibility with amniotic membrane tissues, does not generate adverse effects on the structure and the function of the amniotic membrane, and has higher biological safety.

An apparatus for filtering amniotic fluid containing particulate matter and amniotic cells is disclosed, comprising a filter for filtering the particulate matter from the amniotic fluid, a chamber enclosing the filter, wherein the chamber comprises a fluid inlet and a fluid outlet, and an inlet connector arranged to form a sealing connection between the fluid inlet and an amniotic fluid sample source, wherein, in use, the amniotic fluid pass the filter when flowing from the fluid inlet to the fluid outlet, whereby particulate matter is deposited on the filter and the amniotic fluid flows through the fluid outlet containing amniotic cells.

The present invention relates to a decellularized placenta-derived amniotic membrane-based hydrogel, a use thereof, and a method for preparing same, the hydrogel being characterized by comprising a decellularized placenta-derived amniotic membrane, wherein the content of collagen in the decellularized placenta-derived amniotic membrane is 9 µg / mg to 15 µg / mg, and the content of glycosaminoglycans (GAGs) is 0.3 µg / mg to 1.0 µg / mg.

The utility model belongs to the technical field of amniotic membrane cleaning supports, particularly relates to a simple and convenient support for cleaning amniotic membranes, and aims to solve the problems that in the prior art, the amniotic membranes are manually clamped by tweezers to shake in a plate for multiple times, a plurality of cleaning plates are replaced to repeatedly shake and clean the amniotic membranes, and the large amniotic membranes are difficult to clean. Four foot pads are arranged at the bottom of the base, two stainless steel supports are fixedly mounted at the top of the base, grooves are formed in the inner sides of the two stainless steel supports, openings are formed in the tops of the grooves, and the stainless steel supports are made of stainless steel; the two protruding blocks are connected with the inner walls of the two grooves in a sliding mode. The amniotic membrane cleaning device is simple in structure and convenient to use, an amniotic membrane can be flatly unfolded and vertically erected on the two stainless steel supports, cleaning and using are convenient, the angle of the double rings can be adjusted, and the amniotic membrane cleaning angle can be adjusted.