189 results about "Amniotic Sheet" patented technology

The invention relates to an amniotic membrane mirror manufacturing method which comprises the following steps: S1, performing transparency and shape fixing treatment on an amniotic membrane to obtain a spherical amniotic membrane area; s2, the super oxygen permeable material is subjected to casting plasticity to obtain a transition area and a landing area which are integrated, and the landing area surrounds the transition area; wherein the transition area is aspheric and the vertical projection of the transition area is annular, and the landing area is spherical and the vertical projection of the landing area is annular; s3, a plurality of fluid channels which are arranged in a crossed mode are etched in the top of the transition area, and the multiple fluid channels form a latticed path body extending in the circumferential direction of the transition area; s4, forming a plurality of uniformly arranged nano structures in each grid region of the path main body to form a connection path; s5, the fluid channel is filled with a biocompatible adhesive, the amniotic membrane area is placed on the transition area, and the bottom edge of the amniotic membrane area abuts against the connecting path; the method has the beneficial effects that on the basis of ensuring the biological characteristics of the amniotic membrane area, the stable connection between the amniotic membrane area and the transition area is realized.

The invention discloses a cutting device for amniotic membrane pretreatment, which comprises a supporting assembly, a transmission assembly, a feeding assembly, a cutting assembly and a cutting box, the supporting assembly comprises a supporting leg and a supporting sleeve, the two supporting sleeves are respectively arranged at the tops of the supporting legs in a mirror image manner; the feeding assembly is rotationally arranged on the opposite faces of the two supporting sleeves. The two transmission assemblies are arranged in the two supporting sleeves in a mirroring mode correspondingly, and the transmission assemblies are connected with the moving end of the cutting assembly and the transmission end of the feeding assembly correspondingly. According to the amniotic membrane cutting device, irregular amniotic membranes can be cut, subsequent use and storage of the amniotic membranes are facilitated, the efficiency is high, and the cutting precision is high.

The invention provides an amnion-loaded contact lens and a preparation method and application thereof. According to the invention, the bonding mode of the amniotic membrane and the contact lens is innovated, the previous mode of simply bonding the amniotic membrane and the contact lens by using medical biological glue is changed, the bonding procedure of the amniotic membrane and the contact lens is simplified, and the method is suitable for industrial application; the problems that the mechanical supporting force is reduced, the amniotic membrane is broken and the coverage is uneven due to the fact that the ocular surface coverage time of a traditional suture type amniotic membrane is prolonged are solved; in the aspect of material optimization and selection, the basic requirement of non-toxicity and harmlessness of the adhesive is met, and meanwhile, the requirements of biocompatibility and the oxygen permeability of the cornea on the contact lens are also considered; the amniotic membrane is more comfortable in the using process, complex operation procedures are not needed, and stimulation caused by no suture is avoided; the design scheme that the contact lens is used as the amniotic membrane support enables the amniotic membrane lens to provide better support and protection for the covered ocular surface.

The present invention relates to an incubation system for liquid-based incubation of prematurely born infants, comprising: —an inner chamber forming an amniotic basin comprising amniotic fluid, said basin being configured for holding said infant and being made from a flexible material configured for expanding said inner chamber volume in correspondence with the growth of said infant; —an outer chamber enclosing said inner chamber and comprising a temperature regulation fluid, —a fetal connection port, arranged for connecting with the umbilical cord of said infant, said umbilical cord providing a port in said inner chamber to said infant for providing dialyzation and nutrition compounds to said infant via said umbilical cord; —a fetal control unit, connected to said fetal connection port for control of said dialyzation and control of said provided nutrition by monitoring and controlling one or more of a pressure, flow and temperature thereof; an amniotic fluid circulation unit, arranged for connecting with an inlet / outlet port of said inner chamber and comprising a pump for circulating said amniotic fluid from said inner chamber and through a purification system located outside said inner chamber; and —a temperature regulation fluid control unit, arranged for connecting with an inlet / outlet port of said outer chamber and comprising a pump for circulating said temperature regulation fluid from said outer chamber and through a heat exchanger system located outside said inner chamber.

The invention discloses application of amniotic mesenchymal stem cells in preparation of a medicine for improving and / or treating premature ovarian failure. The invention finds that the amniotic mesenchymal stem cells can significantly improve the granular cell proliferation ability and hormone secretion ability, and the premature ovarian failure animal model proves that the amniotic mesenchymal stem cells have leading treatment effects in the aspects of restoring the ovarian functions (hormone secretion, ovary volume and follicle number of each level) of POF mice and improving fertility again. And a foundation is laid for optimizing a treatment strategy of premature ovarian failure and promoting clinical application of mesenchymal stem cell treatment.

The invention discloses a decellularized amniotic membrane matrix composite scaffold, a preparation method thereof and application of the decellularized amniotic membrane matrix composite scaffold in orbital bone defect repair, and relates to the technical field of biomedical engineering and biomedical materials. In order to solve the limitation of an existing orbit implant material, methacrylic anhydride (MA) is grafted to HAM, methacrylate HAMMA hydrogel is synthesized, and then the decellularized amnion matrix composite scaffold (HAMMA / HAP / BMP-2) is prepared by combining a simulated body fluid biomimetic mineralization method with 3D printing and ultraviolet curing. The composite scaffold material prepared by the invention can regulate the release of BMP-2 and maintain the effective drug concentration of a bone defect area so as to regulate the immune microenvironment of an early bone defect repair area, collect osteoblasts and accelerate the repair of bone defects.

The invention relates to the technical field of tissue repair, in particular to a placenta amnion decellularized material as well as a preparation method and application thereof. The amnion is subjected to decellularization treatment by adopting a high-salt freeze-thaw combined composite enzymolysis method, and the result proves that the amnion decellularization material prepared by the invention is a high-activity bioremediation material, and the natural three-dimensional structure and bioactive components of an amnion extracellular matrix are reserved to the greatest extent; and the prepared filling material has better biocompatibility and tissue inductivity.

The invention discloses an amniotic epithelial stem cell screening method and device based on microscopic image feature extraction, relates to the technical field of microscopic image analysis and cell screening, and is used for solving the problem that group topology and internal activity in amniotic epithelial stem cell lossless screening are difficult to collaboratively quantify. The method comprises the following steps: firstly, performing enhancement processing on a phase difference microscopic image, and extracting a kernel centroid and a global gradient energy diagram; then, constructing a Voronoi jurisdiction, and coupling a cytoplasm texture entropy and a gray scale attenuation gradient to generate a cytoplasm compactness factor; meanwhile, calculating a cell gap topology consistency index based on mapping of the Voronoi boundary and the gradient map; and finally, weighting and correcting the compactness factor by using a topological index to obtain a dryness maintenance confidence coefficient, and outputting a target coordinate. Through dual verification of geometric topology and physical gradient, combined constraint screening of internal and external characteristics of cells is realized.

The invention relates to the technical field of tissue repair, in particular to an amnion decellularized matrix for a filling material and a preparation method of the amnion decellularized matrix. The amnion decellularized matrix with the tissue repair function is separated and prepared from amnion tissue, specific processes such as enzymolysis are adopted in the preparation method of the amnion decellularized matrix, and the content of growth factors, collagen, glycosaminoglycans and other components in the amnion decellularized matrix can be effectively increased; the material can meet the repairing function of different types of tissues, is suitable for being used as a personalized biological filling material, and has a wide application prospect.

A multi-part processed human amniotic composition configured to treat Peyronie's disease in a subject in need thereof by intracorporeal injection of the composition into the corpus cavernosum of the subject to reduce plaque size associated with Peyronie's disease. The multi-part processed human amniotic composition includes a micronized human amnion composition; and an aqueous human amniotic fluid filtrate configured to reconstitute and suspend the micronized human amnion composition therein. In certain aspects, the multi-part processed human amniotic compositions are not processed with exogenous enzymes during production thereof and do not include exogenous enzymes, such as collagenase, added thereto.

The present invention belongs to the technical field of image processing, and discloses a three-dimensional face imaging method, device, equipment and storage medium. The method includes: acquiring original three-dimensional volume data of a tissue to be measured; determining the face position and amniotic membrane position of the tissue to be measured according to the original three-dimensional volume data and a preset threshold; determining a target positioning point profile according to the face position and the amniotic membrane position; performing transparency clearing on the original three-dimensional volume data according to the target positioning point profile to obtain three-dimensional face data of the tissue to be measured; and completing three-dimensional face imaging display of the tissue to be measured according to the three-dimensional face data. By the above method, a target positioning point profile is determined based on the amniotic membrane position and the face position, so as to identify the tissue information before the face of the tissue to be measured. Finally, transparency clearing is performed on the original three-dimensional volume data according to the target positioning point profile to complete three-dimensional face imaging display of the tissue to be measured, thereby automatically obtaining a clear, comprehensive and complete face image of the object to be measured, and improving the image rendering effect.

The present invention relates to an amnionoscope and a fitting method thereof, wherein the amnionoscope comprises an amnion area, a transition area and a landing area; the transition area is connected to the landing area, and in a top-down perspective, the landing area surrounds the transition area; a connection path is provided on the top of the transition area around its circumference, and the amnion area is installed on the transition area through the connection path; the connection path comprises a plurality of fluid channels and a plurality of connection components; the plurality of fluid channels are cross-arranged to form a grid-shaped path body, and the plurality of connection components are located one by one in a plurality of grid areas of the path body; the fluid channel is filled with a biocompatible adhesive, the transition area is connected to the amnion area through the biocompatible adhesive, and the amnion area abuts against the plurality of connection components, and the plurality of connection components form physical adsorption on the amnion area, and the beneficial effect is that the transition area forms a main connection and an auxiliary connection with the amnion area through the connection channel, and a stable connection between the amnion and the transition area is achieved without affecting the biological characteristics of the amnion, thereby ensuring the subsequent use effect of the amnionoscope.

An amniotic membrane insert configured for self-retaining, sutureless, clear-cornea application to an ocular surface. The insert has an outer edge and an inner edge inside the outer edge. The inner edge defines an opening sized to extend around a circumference of a cornea. The outer edge of the insert has a shape that is non-circular in which a height of the insert is greater than a width of the insert. Related devices, systems, and methods are provided.

The utility model belongs to the technical field of membrane rupturing devices, and particularly relates to an amniotic membrane rupturing device for gynaecology and obstetrics, which comprises a handle, one end of the handle is fixedly connected with a membrane rupturing hook, and the membrane rupturing hook is arranged towards the other end of the handle; the front face and the back face of the handle are each provided with a liquid guide groove formed in the length direction of the handle, the handle is provided with penetrating holes corresponding to the liquid guide grooves, and the penetrating holes are all formed in the ends, away from the membrane breaking hooks, of the liquid guide grooves. A handheld part is arranged at one end, far away from the membrane rupture hook, of the handle, and an annular groove is further formed in the handle and is formed between the handheld part and the liquid guide groove; the utility model provides the amniotic membrane rupture device for the obstetrics and gynecology department, which can be used for guiding amniotic fluid.

The present invention belongs to the field of bioengineering technology, and discloses a preparation method and application of an amniotic membrane extract. Fresh amniotic membrane is disinfected, cleaned, and dried in sequence, and then crushed in an ice bath. Then, the crushed liquid is centrifuged and filtered to obtain the amniotic membrane extract. The dry matter of the amniotic membrane extract meets the following requirements: total protein content ≥78.02%, total sugar content ≥10.87%, fat content ≤3.53%, and ignition residue content ≤1.64%. The present invention improves the extraction efficiency of total protein and total sugar in the amniotic membrane extract by changing the existing production process of frozen crushing or liquid crushing, crushing the amniotic membrane in an ice-water mixture, and combining the control of process parameters such as crushing, which is beneficial to the promotion and application of the amniotic membrane extract in the preparation of ocular preparations such as ocular surface lubricating liquid, and improves the clinical use effect. In addition, the amniotic membrane extract can also be used as a functional component of biological amniotic membrane preservation liquid and combined with amniotic membrane covering surgery for the treatment of ocular surface diseases.

The invention provides an amniotic membrane mirror with low foreign body sensation and a preparation method thereof.The amniotic membrane mirror is composed of a large ring, a small ring and a biological amniotic membrane, the inner side of the large ring is provided with a weft groove and a warp groove, the biological amniotic membrane is located between the large ring and the small ring, the small ring is embedded into the weft groove of the large ring to clamp and fix the biological amniotic membrane to the inner side of the large ring, and the small ring is embedded into the warp groove of the large ring to clamp and fix the biological amniotic membrane to the inner side of the large ring. The large ring is made of soft materials, the small ring is made of hard materials, after the biological amniotic membrane is clamped and fixed through the large ring and the small ring, a patient wears the biological amniotic membrane in an amniotic membrane mirror mode, the large ring with the largest contact area with the ocular surface is made of the soft materials, foreign body sensation after wearing can be greatly reduced, and the biological amniotic membrane has wide application prospects and is beneficial to application and popularization.

The invention discloses a low foreign body sensation imbibition split type biological amniotic membrane bandage mirror and a preparation method thereof.The low foreign body sensation imbibition split type biological amniotic membrane bandage mirror comprises an amniotic membrane supporting frame, an amniotic membrane fixing ring and an amniotic membrane, the amniotic membrane fixing ring is made of a soft material, the amniotic membrane supporting frame comprises an amniotic membrane supporting area, a landing area, a pressing attachment area and an imbibition fixing ring, and the amniotic membrane supporting area is made of a hard material; the landing area and the pressing attachment area are both made of soft materials, the amniotic membrane supporting area is used for supporting the landing area and the pressing attachment area, the lower end of the pressing attachment area is fixedly connected with the upper end of the amniotic membrane supporting area, the upper end of the landing area is fixedly connected with the lower end of the amniotic membrane supporting area, and the amniotic membrane is attached to the inner surface and the outer surface of the amniotic membrane supporting frame and fixed through the amniotic membrane fixing ring. The device can reduce contact with the cornea, prevent the cornea from being crushed, reduce oppression, reduce foreign body sensation, improve comfort, prevent pus from flowing back to infect the cornea, is beneficial to treatment and recovery of the cornea, is easy to operate, can be directly removed from eyes, does not need too many auxiliary instruments, and greatly reduces the infection probability.

The application provides an amniotic membrane stem cell and a separation method and a hydrogel 3D culture method thereof, relates to the technical field of cell culture, and comprises the following steps: placing amniotic membrane fragments in a pre-digestion buffer in sequence and incubating for 10-20 min, placing the amniotic membrane fragments in a mixed enzyme solution and performing digestion treatment, filtering, and obtaining a filtrate and residual amniotic membrane fragments; centrifuging the filtrate to obtain a cell precipitate, and performing differential adhesion culture to obtain amniotic membrane epithelial stem cells; adding a culture medium to resuspend the residual amniotic membrane fragments so that the residual amniotic membrane fragments are in contact with a culture container and are in a non-suspended state, performing adhesion culture, and obtaining amniotic membrane mesenchymal stem cells that have climbed out. Compared with the number of cells and the cell survival rate of cells that are subjected to digestion treatment of other mixed enzymes, the number of cells obtained by digestion treatment of the mixed enzyme is significantly increased, and the cell survival rate is higher; compared with a step-by-step digestion strategy, the mixed enzyme digestion one-step method is more simple, and efficient and synchronous separation is achieved. The technical problem of a significant decrease in cell activity caused by the step-by-step digestion separation strategy in the prior art is solved.

The invention belongs to the technical field of biological medicines, and particularly relates to amniotic mesenchymal stem cells for treating gonitis as well as a preparation method and application of the amniotic mesenchymal stem cells. The preparation method of the amniotic mesenchymal stem cells comprises the following specific steps: (1) separating to obtain amniotic tissues; (2) digesting, filtering and centrifuging the amniotic membrane tissues to obtain cell precipitates; (3) precipitating and resuspending the cells, inoculating the cells to a culture medium for culture, digesting, centrifuging, and discarding supernatant to obtain P0-generation amniotic mesenchymal stem cells; and (4) inoculating the P0-generation amniotic mesenchymal stem cells into a culture medium for subculture to obtain the amniotic mesenchymal stem cells. By adding teprenone and schaftoside into the basic culture medium, the cell dryness of the amniotic mesenchymal stem cells can be maintained, and the osteogenic differentiation capability of the amniotic mesenchymal stem cells is improved, so that the gonitis is effectively treated. Meanwhile, fetal calf serum is not added into the basic culture medium, so that the safety of clinical application is improved.

A cartridge configured to be connected to a surgical stapler includes an anvil leg having an inwardly facing surface and a staple leg pivotally connected to the anvil leg. The staple leg includes a plurality of staples and an inwardly facing surface configured to move towards the inwardly facing surface of the anvil leg. The inwardly facing surface of the staple leg includes a plurality of openings positioned for the plurality of staples to pass through the plurality of openings to tissue positioned between the anvil leg and the staple leg. The cartridge also includes a cover device having at least one sheet of amnion attached to the inner surface of at least one of the anvil leg or the staple leg configured to attach to the tissue positioned between the inwardly facing surface of the anvil leg and the inwardly facing surface of the staple leg.

The present invention provides an amniotic membrane-loaded contact lens, its preparation method, and its application. This invention innovates the bonding method between amniotic membrane and contact lens, replacing the previous method of simply bonding the amniotic membrane and contact lens with medical biological glue. This simplifies the bonding process between amniotic membrane and contact lens, making it suitable for industrial application. It also addresses the issues of traditional sutured amniotic membranes, which suffer from decreased mechanical support, rupture, and uneven coverage over time as they cover the ocular surface. The optimization and selection of materials not only meet the basic requirements of a non-toxic and harmless adhesive, but also take into account biocompatibility and the cornea's requirements for contact lens oxygen permeability. The use of amniotic membranes is more comfortable, eliminating the need for complex surgical procedures and the irritation associated with sutures. The design of using a contact lens as an amniotic membrane support allows amniotic lenses to provide superior support and protection for the covered ocular surface.

The invention relates to the technical field of medical tools, in particular to an artificial membrane rupturing structure which comprises a barrel and a suction cup mounted at the top end of the barrel and further comprises a guide seat, a membrane rupturing device, a membrane rupturing device and a membrane rupturing device, the guide seat is mounted in the barrel, and a through hole is formed in the guide seat; the adsorption mechanism is installed in the cylinder body and connected with the guide seat, and the adsorption mechanism enables the suction cup to be adsorbed to the surface of the amniotic membrane; the artificial membrane rupturing structure is different from the prior art, in the using process of medical staff, the amniotic membrane can be clamped and fixed only by attaching the suction cup to the amniotic membrane and then pulling the hollow push rod, then puncturing is automatically conducted, the flow speed after membrane rupturing is stable, the membrane rupturing can be easily completed even though the medical staff with less experience, and operation is easy; the amniotic fluid after rupture of the membrane automatically flows to the outside through the drainage tube from the inside of the cylinder, and the amniotic fluid is not in contact with the uterus of the pregnant woman, so that the probability of infection in the uterus of the pregnant woman is greatly reduced, and the turbidity of the amniotic fluid flowing out can be directly observed.

The invention discloses a method for promoting human amniotic mesenchymal stem cells to differentiate into vascular endothelial cells. The method comprises the following steps: preparing an electrostatic spinning film as a cell culture substrate; planting human amniotic mesenchymal stem cells (hAMSC) on the electrostatic spinning film; adding a vascular endothelial growth factor (VEGF) into the culture medium to induce hAMSC differentiation; and maintaining the cell culture until the characteristic expression of the vascular endothelial cells. The polylactic acid-polycaprolactone copolymer (PLCL) is used as the material of the electrostatic spinning film, and the film prepared by the electrostatic spinning technology has a unique three-dimensional reticular fiber structure, so that a growth environment closer to a natural extracellular matrix is provided for cells. The structure can effectively promote adhesion and proliferation of human amniotic mesenchymal stem cells (hAMSC), and differentiates to vascular endothelial cells under the induction of vascular endothelial growth factors (VEGF), so that the differentiation efficiency is remarkably improved.

The utility model discloses an amniotic cavity constant-speed perfusion device which effectively solves the problem that an existing amniotic cavity perfusion tool cannot conduct constant-speed perfusion. Comprising an up-down axial main body column, a left-right supporting rod is arranged at the upper end of the main body column, an up-down through sliding groove is formed in the supporting rod, a positioning shell is slidably connected into the sliding groove, an up-down axial up-down through needle placing hole is formed in the upper end of the positioning shell, and a puncture needle is detachably connected into the needle placing hole; an up-down axial containing groove is formed in the upper end of the main body column, a piston cylinder with a downward opening is coaxially arranged in the containing groove, a piston plate capable of moving up and down is coaxially arranged in the piston cylinder, and the upper end of the piston cylinder is connected with the upper end of the puncture needle through a hose; the amniotic cavity puncture infusion device can achieve puncture infusion of an amniotic cavity of a patient, relieve the phenomenon that the amniotic fluid of the patient is too little, keep constant-speed uniform infusion in the whole infusion process, and prevent the situation that the infusion speed is too high or too low, and consequently the patient is hurt.

The present invention discloses a decellularized amniotic membrane material, a preparation method thereof, and an application thereof in the preparation of an ocular surface repair material. The preparation method comprises the following steps: (1) taking a placenta, separating the amniotic membrane from the placenta, and then using a cell scraper to remove excess tissue on the surface of the amniotic membrane to obtain a chorionic amniotic membrane; (2) washing the chorionic amniotic membrane treated in step (1) to obtain a washed chorionic amniotic membrane; (3) placing the washed chorionic amniotic membrane in a decellularizing reagent for decellularization to obtain a decellularized amniotic membrane; (4) washing the decellularized amniotic membrane, and then placing it in an ethanol solution containing a natural extract for soaking treatment; (5) taking out the decellularized amniotic membrane treated in step (4), and then drying, shearing, and irradiating and sterilizing it to obtain the decellularized amniotic membrane material. Studies have shown that the decellularized amniotic membrane material prepared by the method of the present invention has a good effect of promoting cell proliferation.

The invention relates to the technical field of obstetrical nursing, in particular to an obstetrical nursing membrane rupture midwifery device with a negative pressure balancing function, which comprises an inserting cylinder, an endoscope is fixedly mounted at the upper part of the rear end in the inserting cylinder, and a membrane rupture assembly is arranged at the rear end in the inserting cylinder. The film breaking assembly comprises a partition plate fixed to the rear end of the interior of the inserting barrel, a plurality of film guiding holes are formed in the partition plate, a cutter groove is formed in the rear end face of the partition plate, and a film breaking blade is movably installed in the cutter groove. According to the amniotic membrane rupturing device, a baffle can block a fetus in an amniotic membrane, so that the soft outer wall of the amniotic membrane enters a membrane guiding hole, the fetus in the amniotic membrane cannot be damaged during rupture of the amniotic membrane, and meanwhile, a membrane rupturing blade is located in an inserting cylinder, so that when rupture of the amniotic membrane is carried out, the fetus can be ruptured conveniently. And the membrane rupture blade does not hurt a fetus or a puerpera.

The invention relates to the technical field of medical instruments, in particular to a frameless amniotic membrane mirror device, a manufacturing method and a using method, and the frameless amniotic membrane mirror device comprises an amniotic membrane tissue layer and a biocompatible thin film layer formed by spraying a mixed solution of polymethyl acrylate and polyvinyl alcohol; the overall diameter of the device is 14 mm, the rise of the device is 6.2 + / -0.1 mm, and the device can be directly implanted into the ocular surface to cover the cornea without a frame support. The manufacturing method comprises the steps of amniotic membrane treatment, model eye forming, mixed solution preparation, membrane spraying and forming, trimming and sterilization. The application method relates to implantation after surface anesthesia, fitting observation and subsequent taking-out. Through the frameless conformal fitting design, the problems that an existing framed amniotic membrane mirror is displaced and cannot be worn by a patient with strong foreign body sensation and shallow conjunctival sac are solved, and meanwhile secondary trauma of a suture operation is avoided. And the residual film after the amniotic membrane is dissolved can continuously provide protection, and the amniotic membrane has the advantages of good fitness, high patient comfort, simplicity and convenience in operation and good safety.

This disclosure generally relates to grafts having exudate control for treating a wound of a subject and methods thereof. In some embodiments, the grafts comprising a first layer comprising a first portion and a second portion. In some embodiments, the first portion comprises amnion, chorion, or a combination thereof. In some embodiments, the second portion comprises a swellable hydrogel. In other embodiments, the grafts comprise a first layer comprising amnion, chorion, or a combination thereof and a second layer, disposed on the first layer, comprising the swellable hydrogel. Additionally, in some embodiments, the grafts may further comprise one or more additional layers, for example, a third layer, adjacent the second layer, comprising an adsorbent material; and / or an adhesive fourth layer adjacent the third layer for adhering said graft to the skin of a subject. Other embodiments are directed toward methods of bonding two or more layers within the graft.

A placental collagen matrix (PCM) that can be poured into a customized tray shape and then dehydrated that can be used in the treatment of wounds. Specifically, the PCM has the property of being able to be poured into a variety of shapes, sizes and thickness. The resulting customized graft may be used in the treatment of a variety of wounds. The PCM may or may not be decellularized, and may be made of placental disc, umbilical cord, amnion, chorion, and / or intermediate layer.