Amnion stroma tectorial blood vessel internal branch and preparing method thereof

A technology of inner stent and matrix, which is applied in the field of medical devices, can solve problems such as human injury, and achieve the effect of overcoming limited sources

Inactive Publication Date: 2007-03-14
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to develop a new biofilm vascular stent, aiming at the current situation that the source of biofilm stent coating material is limited and the source path may cause certain d...

Method used

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  • Amnion stroma tectorial blood vessel internal branch and preparing method thereof
  • Amnion stroma tectorial blood vessel internal branch and preparing method thereof
  • Amnion stroma tectorial blood vessel internal branch and preparing method thereof

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Embodiment Construction

[0025] Step 1: Preparation of Amniotic Membrane Matrix

[0026] ①Acquisition of amniotic membrane: the amniotic membrane is derived from the placental tissue of caesarean section women who are negative for hepatitis virus antibody, syphilis antibody and HIV. It is bluntly separated through the potential gap between the amniotic membrane and the chorion, washed with normal saline for 3 times, and then washed with 0.1% Soak in gentamicin and amphotericin B balanced salt solution for 15 minutes, peel off the amniotic membrane and chorion, spread the epithelial side up on the nitrocellulose membrane, and obtain smooth, transparent, and avascular amniotic membrane with pure glycerol 4 ℃ preservation method.

[0027] ② "Decellularization" treatment of amniotic membrane:

[0028] i) Digestion with 0.25% trypsin: put it into an electric thermostatic water bath at 37° C. for 15 minutes, and scrape off the residual epithelial cells.

[0029] Or ii) Digestion with 0.25% trypsin + 0.02%...

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Abstract

The invention relates to an amnion substrate covered blood vessel internal support and relative production. Wherein, it covers and decorates the blood vessel internal support and plants the endothelium cell, to avoid narrow condition; the support is formed by blood vessel internal support, amnion substrate covering the support, and the endothelium cell planted on the substrate. And the production comprises that removing cells of amnion, to be biological film with substrate film and dense layer; covering and decorating it on the blood vessel internal support, to form smooth and flat film on the inner surface; then planting the endothelium cell, to form a liner, as one inner layer of blood vessel sample, and approach the surface of support to the inner wall of vessel, to increase the bioavailability and blood compatibility.

Description

technical field [0001] The invention relates to coating modification and endothelialization treatment of intravascular stents, and provides a new idea for preventing and even solving the restenosis problem caused by intravascular stent implantation, and belongs to the technical field of medical devices. Background technique [0002] Cardiovascular and cerebrovascular diseases are a large class of diseases that seriously endanger human health. They are called the "number one killer" in developed countries. It is an important means and is widely used at home and abroad. However, the stent itself is coagulative, and after several months of implantation, there is still a restenosis rate of 25-30%, and the restenosis rate can be as high as 40% within one year, which is still an unsolved problem. Therefore, preventing restenosis after stent implantation has become an important research direction at present. Many related experimental studies have been carried out at home and abro...

Claims

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Application Information

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IPC IPC(8): A61L27/14A61L27/38A61L27/44A61L27/00A61F2/82
Inventor 王贵学赵宏伟罗来龙江涛
Owner CHONGQING UNIV
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