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Subculture method of tamarix chinensis tissue culture seedling

A technology of subculture and subculture medium, applied in the field of subculture of Tamarisk tissue culture seedlings, can solve the problems such as browning of the base of tissue culture seedlings, endangering survival, proliferation speed and rooting rate, etc., so as to avoid browning. Effect

Inactive Publication Date: 2018-05-11
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This phenomenon did not appear in the process of explant induction, but it would occur after being transferred to the rooting medium. The tissue culture seedlings with browning phenomenon could still grow and take root, but the proliferation rate and rooting rate were significantly affected. In severe cases, the base of tissue culture seedlings will gradually brown and endanger their survival.

Method used

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  • Subculture method of tamarix chinensis tissue culture seedling

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Comparison scheme
Effect test

Embodiment 1

[0031] The invention discloses a method for subculture of tamarix tissue cultured seedlings. The specific steps are as follows:

[0032] (1), branch acquisition: take the new twigs of the Tamarix plant, and use clear water to wash the twigs, and the washing time is 2 to 3 hours;

[0033] (2), branch sterilization: place the twig after washing in (1) in a concentration of 70% to 75% alcohol for disinfection for 1 to 2 minutes, then rinse with sterile water for 3 to 4 times;

[0034] (3), primary tissue acquisition: cut the epidermis of the twig to obtain the internal tissue:

[0035] (4) Primary tissue culture: place the primary tissue in a vessel containing the primary culture medium, and cultivate it at room temperature at 25°C under light conditions. After culturing for a period of time, the primary tissue cells inside the vessel divide The secondary tissue cells are produced, and with the division of the tissue cells, part of the secondary tissue cells are suspended in the...

Embodiment 2

[0042] The invention discloses a method for subculture of tamarix tissue cultured seedlings. The specific steps are as follows:

[0043] (1), branch acquisition: take the new twigs of the Tamarix plant, and use clear water to wash the twigs, and the washing time is 2 to 3 hours;

[0044] (2), branch sterilization: place the twig after washing in (1) in a concentration of 70% to 75% alcohol for disinfection for 1 to 2 minutes, then rinse with sterile water for 3 to 4 times;

[0045] (3), primary tissue acquisition: cut the epidermis of the twig to obtain the internal tissue:

[0046] (4) Primary tissue culture: place the primary tissue in a vessel containing the primary culture medium, and cultivate it at room temperature at 25°C under light conditions. After culturing for a period of time, the primary tissue cells inside the vessel divide The secondary tissue cells are produced, and with the division of the tissue cells, part of the secondary tissue cells are suspended in the...

Embodiment 3

[0053] The invention discloses a method for subculture of tamarix tissue cultured seedlings. The specific steps are as follows:

[0054] (1), branch acquisition: take the new twigs of the Tamarix plant, and use clear water to wash the twigs, and the washing time is 2 to 3 hours;

[0055] (2), branch sterilization: place the twig after washing in (1) in a concentration of 70% to 75% alcohol for disinfection for 1 to 2 minutes, then rinse with sterile water for 3 to 4 times;

[0056] (3), primary tissue acquisition: cut the epidermis of the twig to obtain the internal tissue:

[0057] (4) Primary tissue culture: place the primary tissue in a vessel containing the primary culture medium, and cultivate it at room temperature at 25°C under light conditions. After culturing for a period of time, the primary tissue cells in the vessel divide The secondary tissue cells are produced, and with the division of the tissue cells, part of the secondary tissue cells are suspended in the int...

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Abstract

The invention discloses a subculture method of a tamarix chinensis tissue culture seedling. In the culture process, quinone type substances released by a plant body can be adsorbed by activated carbon, and contact inhibition growth happens among cells when subculture is conducted on subculture tissue cells and the subculture tissue cells divide; meanwhile, due to the insufficient nutrients and metabolite accumulation in a subculture medium, a cell culture medium needs to be replaced regularly, the quinone type substances released by the plant body during culture can be poured together, and thecultured seedling is prevented from browning.

Description

technical field [0001] The invention relates to the field of subculture of tamarix planting, in particular to a method for subculture of tamarix tissue cultured seedlings. Background technique [0002] Tamarix is ​​also known as weeping silk willow, Xihe willow, West Lake willow, red willow, and Yin willow; the tender branches and leaves of Tamarix are Chinese medicinal materials, which are produced in various parts of China and are used fresh or dried. Tamarix has delicate branches, whirling posture, and blooms like Polygonum rubrum. It is quite beautiful and is often planted as an ornamental plant in gardens. It can be used for poor penetration of pox or rash, cold, cough, rheumatism and bone pain. At present, the methods of Tamarix cultivation include methods such as container seedling cultivation, branch cutting and subculture. [0003] In the prior art, when Tamarix is ​​cultured in vitro, the quinones released by the plant make the medium around the plant turn brown,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨庆山周健刘德玺魏海霞李永涛王霞王莉莉王守国朱文成董玉峰
Owner SHANDONG FOREST SCI RES INST
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