Triploid loach fin cell line TRMF and establishing method thereof

A triploid, fin cell technology, applied in the direction of animal cells, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of no triploid loach fin cell line and so on

Inactive Publication Date: 2014-01-22
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, to date there are no reports of triploid loach fin cell lines

Method used

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  • Triploid loach fin cell line TRMF and establishing method thereof
  • Triploid loach fin cell line TRMF and establishing method thereof
  • Triploid loach fin cell line TRMF and establishing method thereof

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Embodiment Construction

[0021] The fin cell line of the triploid loach of the present invention and its establishment method are described below through specific embodiments.

[0022] 1. Establishment of cell lines

[0023] The triploid loach used was obtained by crossing diploid loach and tetraploid loach.

[0024] The establishment process of the triploid loach fin cell line TRMF includes the following three stages.

[0025] (1) Preparation of cell culture medium

[0026] The medium used is commercially available DMEM / F12 medium, which is prepared by adding fetal bovine serum (FBS), human basic fibroblast growth factor, type I insulin-like growth factor and chondroitin sulfate to prepare a cell culture medium. Among them, fetal bovine serum accounts for 20% of the total volume of the culture medium, the final concentration of human basic fibroblast growth factor is 10 ng / mL, the final concentration of type I insulin-like growth factor is 20 ng / mL, and the final concentration of chondroitin sulfat...

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Abstract

The invention provides a triploid loach fin cell line TRMF and an establishing method of the triploid loach fin cell line TRMF. The triploid loach fin cell line TRMF is preserved in the China Typical Culture Collection Center on August 19, 2013 with the preservation number C2013110. The triploid loach fin cell line TRMF is susceptive to a virus of spring viraemia of a carp. The biological characteristics of the triploid loach fin cell line TRMF comprise that cells are like fibroblasts, cytoplasm is rich and grows attaching to cell walls, the cells have the contact inhibition growth characteristic, the cell doubling time is 36.01 hours, and the number of feature chromosomes is 75.

Description

technical field [0001] The invention relates to a triploid loach fin cell line TRMF and its establishment method. Background technique [0002] Loach (Mi s gurn us anguilli Caudatus) belongs to Cypriniformes (Cypriniformes), loach family (Cobitidae), flower loach subfamily (Cobitinae), loach genus (Misgurnus) in taxonomy, and is a wild small freshwater fish endemic to Asia. , widely distributed in most areas south of the Liaohe River in my country, as well as in Vietnam, Korea and Japan. It is distributed in various freshwater waters in my country, such as lakes, ponds, rivers, ditches, and rice fields. Loach meat is tender, delicious, and rich in nutrition. It is known as "ginseng in water". In addition to its high edible value, it also has certain nourishing and medicinal effects, and is one of the best-selling special aquatic products in the market. In addition to its important economic value, loach is also a good research material in cytology and genetics. Compared wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07C12R1/91
Inventor 李霞马辰秦艳杰李雅娟杨艳津
Owner DALIAN OCEAN UNIV
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