Tetraploid loach fin cell line TEMF and establishment method thereof

A technology of tetraploid loach and fin cells, applied in artificial cell constructs, animal cells, vertebrate cells, etc.

Inactive Publication Date: 2014-02-05
DALIAN OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research on tetraploid loach mainly involves hybridization with diploid to produce tripl

Method used

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  • Tetraploid loach fin cell line TEMF and establishment method thereof
  • Tetraploid loach fin cell line TEMF and establishment method thereof
  • Tetraploid loach fin cell line TEMF and establishment method thereof

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Embodiment Construction

[0022] The tetraploid loach fin cell line of the present invention and its establishment method are described below through specific embodiments.

[0023] 1. Establishment of cell lines

[0024] The tetraploid loach fin cell line TEMF was established by the following steps.

[0025] (1) Preparation of cell culture medium

[0026] The medium used is commercially available DMEM / F12 medium, which is prepared by adding fetal bovine serum (FBS), human basic fibroblast growth factor, type I insulin-like growth factor and chondroitin sulfate to prepare a cell culture medium. Among them, fetal bovine serum accounts for 20% of the total volume of the culture medium, the final concentration of human basic fibroblast growth factor is 10ng / ml, the final concentration of type I insulin-like growth factor is 20ng / ml, and the final concentration of chondroitin sulfate 10 μg / ml. The prepared culture solution was stored at 4°C.

[0027] (2) Primary culture

[0028] The fin tissues of tetr...

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Abstract

The invention provides a tetraploid loach fin cell line TEMF and an establishment method thereof. The cell line is collected in the China Center for Type Culture Collection (CCTCC) on August 19th, 2013, and the collection number is CCTCC No:C2013111. The tetraploid loach fin cell line TEMF supports the growth and replication of spring viraemia of carp virus. The biological characteristics of the tetraploid loach fin cell line TEMF include fibroblast-like cell, rich cytoplasm and adherent growth and contact inhibition growth; the cell doubling time is 41.45 hours; the number of characteristic chromosomes is 100.

Description

technical field [0001] The invention relates to a tetraploid loach fin cell line TEMF and its establishment method. Background technique [0002] Loach (Misgurnus anguillicaudatus) belongs to Cypriniformes, Cobitidae, Cobitinae, and Misgurnus in taxonomy. It is a wild small freshwater fish unique to Asia and widely distributed in my country. Most of the areas south of the Liaohe River, as well as Vietnam, North Korea and Japan. It is distributed in various freshwater waters in my country, such as lakes, ponds, rivers, ditches, and rice fields. Loach meat is tender, delicious, and rich in nutrition. It is known as "ginseng in water". In addition to its high edible value, it also has certain nourishing and medicinal effects, and is one of the best-selling special aquatic products in the market. In addition to its important economic value, loach is also a good research material in cytology and genetics. [0003] There is polyploidy in loach, and natural tetraploid accounts fo...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12R1/91
Inventor 李霞马辰秦艳杰李雅娟杨艳津
Owner DALIAN OCEAN UNIV
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