A human placental site trophoblastic tumor cell line

A technology of trophoblasts and tumor cells, applied to embryonic cells, animal cells, tumor/cancer cells, etc.

Active Publication Date: 2020-12-08
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that the establishment of tumor cell lines plays an important role in the basic research of tumors, but there is no universal PSTT cell line in the world at present, the inventors of the present application intend to establish a placental site trophoblastic tumor cell line in order to further explore the placenta Etiology, metastasis mechanism, drug resistance mechanism and clinical intervention of site trophoblastic tumor, etc.

Method used

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  • A human placental site trophoblastic tumor cell line
  • A human placental site trophoblastic tumor cell line
  • A human placental site trophoblastic tumor cell line

Examples

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Embodiment 1

[0038] Example 1 Preparation of Human Placental Site Trophoblastic Tumor Cell Line PSTT-1

[0039] (1) Materials: A fresh resection specimen of placental site trophoblastic tumor was obtained from the Obstetrics and Gynecology Hospital of Fudan University in September 2014 (female, 29 years old, placental site trophoblastic tumor, pathological diagnosis: {whole uterus} uterine placental site trophoblastic tumor cell tumor), take 1cm of lesion tissue 3 Immerse in sterile Hanks solution to clean and remove connective and necrotic tissue;

[0040] (2) Primary culture

[0041] Cut the tumor tissue into about 1mm 3 Inoculate the tissue block evenly on the wall of the T25 culture bottle, culture it statically, change the medium every 3 days in half, and observe the cells growing out of the tissue block after about 3 weeks;

[0042] (3) Cell purification

[0043] About 7 weeks after inoculation, pass the first generation, digest with 0.25% trypsin, inoculate the cell suspension i...

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Abstract

The invention belongs to the field of microbial animal cell lines, and in particular relates to a human placental site trophoblastic tumor cell line and a constructing method of the human placental site trophoblastic tumor cell line. Through the in-vitro primary culture on the focus in the uterus of patients suffering from placental site trophoblastic tumor, the human placental site trophoblastic tumor cell line is established; the human placental site trophoblastic tumor cell line is named PSTT-1, and is assigned with the accession number of NO.10882. The human placental site trophoblastic tumor cell line has the biological characteristics that the cells grow in single layer in a wall-attaching manner, the contact inhibition is eliminated, the state is nonuniform, and the cells are in the star-shaped or fusiform-shaped flat structure with multiple bulges; the growth in in-vitro culture is good, and the stable and continuous passage for more than 30 generations can be realized; the karyotype analysis result is the normal female karyotype: 46, XX. The PSTT-1 cell line can be used for the research on the nosetiology, the transfer mechanism, the drug-resistant mechanism and the like of the placental site trophoblastic tumor diseases.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, and in particular relates to the establishment of a human placenta part trophoblastic tumor cell line and its establishment method. Background technique [0002] Studies have reported that placental site trophoblastic tumor (PSTT) is a special type of gestational trophoblastic neoplasm (Gestational trophoblastic neoplasia, GTN), which originates from the intermediate trophoblastic cells of the placental planting site; this disease is relatively rare in clinical practice , accounting for about 1% to 2% of gestational trophoblastic tumors. [0003] Studies have shown that most placental site trophoblastic tumors occur in reproductive age, and the main symptoms are irregular vaginal bleeding or menorrhagia after amenorrhea. According to literature reports, the clinical course of PSTT is mostly benign, but about 10% to 15% of the cases are aggressive. Studies have shown that most of the l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12R1/91
CPCC12N5/0693C12N2500/30C12N2500/84C12N2506/025C12N2509/00
Inventor 康玉刘齐雨李可卢冲蒲萄张明星刘海鸥罗建民李桂玲徐丛剑
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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