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The application discloses a preparation method of a nanofiltration membrane with high chlorine resistance and pollution resistance. The nanofiltration membrane is prepared by the following steps: on the basis of an activated polytetrafluoroethylene microporous membrane, sequentially performing impregnation of an amine monomer and an acyl chloride monomer, then performing heat treatment, then grafting an amine group containing cyclic substance, and finally introducing a quaternary ammonium modification. The nanofiltration membrane prepared by the method has a magnesium sulfate retention rate of 98.0% after exposure to 24000 ppm.h of active chlorine, and the water flux recovery rate of bovine serum albumin is 95.0%, which is almost unchanged compared with that before exposure. It is shown that the nanofiltration membrane has excellent chlorine resistance and good pollution resistance.