Recombinant akabane virus capsid protein, its preparation method and uses

A technology of nucleocapsid protein and Akabane virus, which is applied in the field of genetic engineering, can solve the problems of high technical equipment requirements, cumbersome operation, maternal antibody interference, etc., and achieve the effect of easy purification and preparation, low cost and guaranteed specificity
CN1769294AInactive Publication Date: 2006-05-10HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2006-05-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a novel recombinant Akabane virus nucleotide capsid protein, gene encoding the recombinant protein and the process for preparing the recombinant protein. The recombinant protein has the amino acid sequence represented by SEQ ID No:1, the gene encoding the recombinant Akabane virus nucleotide capsid protein has the amino acid sequence represented by SEQ ID No:2. The preparing process of the recombinant protain comprises the following steps: constructing recombinant Akabane virus nucleotide capsid protein prokaryotic expression vectors, transforming bacillus coli with the recombinant Akabane virus nucleotide capsid protein prokaryotic expression vectors, inducing recombinant nucleotide capsid protein expression with IPTG, reclaiming and purifying the expressed recombinant nucleotide capsid protein.
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Description

technical field

[0001] The present invention relates to a recombinant protein, in particular to a recombinant Akabane virus nucleocapsid protein, a gene encoding the recombinant Akabane virus nucleocapsid protein, a preparation method of the recombinant Akabane virus nucleocapsid protein and its application in detecting or diagnosing Akabane virus The application of the antigen belongs to the field of genetic engineering. Background technique

[0002] Akabane virus, also known as Akabane virus (AKAV), is an arbovirus belonging to the Simpovirus group of the genus Kobney virus, which can cause congenital malformations in cattle, sheep, and goats. Early, stillbirth and congenital arthrogryposis and hydrocephalus anencephaly (Arthrogryposis-Hydraencephaly, AH syndrome) is characterized. The AKAV virus was first isolated from the mosquitoes Cules Tritaeniorhynchis and AedesVexans collected in the cowshed of Akabane Village, Gunma County, Japan in 1959, so it was named Akabane v...

Claims

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