Primer group for rapid detection of 2019-nCoV based on CRISPR technology and application thereof

A 2019-ncov, detection primer technology, used in microorganism-based methods, recombinant DNA technology, and microbial assay/inspection. The effect of advantage
CN111363847AActive Publication Date: 2020-07-03广州微远医疗器械有限公司 +4
5 Cites 13 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
广州微远医疗器械有限公司
Publication Date
2020-07-03

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a primer group for rapid detection of 2019-nCoV based on a CRISPR technology and application thereof, and belongs to the technical field of gene detection of the CRISPR technology. The primer group comprises an Orf1ab gene amplification primer pair, an Orf1ab gene crRNA, an N gene amplification primer pair and an N gene crRNA. The primer group is adopted to detect 2019-nCoV by the CRISPR technology, the detection time of 2019-nCoV is shortened, and detection can be completed within 40-60min. A specific sequence combination is obtained through screening to serve as theprimer group for detection, the primer group has the advantages of being high in sensitivity and specificity, and the detection limit can reach 7.5copies. The primer group is adopted to conduct CRISPRdetection on 2019-nCoV, dependence on complex variable-temperature amplification instruments such as a qPCR instrument is eliminated, and the CRISPR-Cas technology has wide application prospects in the aspect of real-time diagnosis of 2019-nCoV.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of genetic detection based on CRISPR technology, in particular to a primer set for rapid detection of 2019-nCoV based on CRISPR technology and its application. Background technique

[0002] Coronaviruses are a large class of RNA viruses. There are currently seven known coronaviruses that can infect humans. Four of these coronaviruses, including Human coronavirus 229E, Human coronavirus OC43, Human coronavirus NL63, and Human coronavirus HKU1, are more common in the population, but are less pathogenic and generally only cause mild respiratory syndrome similar to the common cold. Two other viruses, severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome coronavirus (MERS), can cause severe respiratory illness. 2019-nCoV is the seventh human-infecting coronavirus discovered so far.

[0003] 2019-nCoV is a betacoronavirus similar to MERS and SARS, both of which originated in bats. The in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More