Cadmium absorption regulation gene, protein, and rice plant having reduced cadmium absorption

A protein and gene technology, applied in the direction of genetic engineering, plant genetic improvement, testing of pharmaceutical preparations, etc., to achieve the effect of high practicability

Active Publication Date: 2014-07-09
NAT INST FOR AGRO ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the existing methods are concerned, there are still many problems in terms of the required number of years, cost and effect

Method used

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  • Cadmium absorption regulation gene, protein, and rice plant having reduced cadmium absorption
  • Cadmium absorption regulation gene, protein, and rice plant having reduced cadmium absorption
  • Cadmium absorption regulation gene, protein, and rice plant having reduced cadmium absorption

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119]

[0120] (heavy ion beam irradiation)

[0121] Rice (variety Koshihikari) seeds (this seed is the first generation) irradiated with heavy ion beams (carbon ions, 320MeV, 40Gy) using TIARA Seeds, hereinafter referred to as M1 for short, and the 2nd, 3rd generations, etc. are referred to as M2, M3, etc.) 3500 grains were sown in culture soil (manufactured by Sumitomo Chemical Co., Ltd., trade name: Bonsol No. 1), and the seedlings obtained were individually The soil was transplanted to the paddy field owned by the (Independent) Agricultural Environmental Technology Research Institute, and M2 seeds were obtained from each individual through the routine cultivation management of the (Independent) Agricultural Environmental Technology Research Institute. About 100,000 of the obtained M2 seeds were all mixed, and tested in the following screening step for low Cd mutants.

[0122] (The screening method for low Cd mutants is a simple screening method based on its 1-ductal Cd...

Embodiment 2

[0133]

[0134] (Evaluation under hydroponic cultivation conditions)

[0135] M3 system seeded with low-Cd mutants (#3-6-4, #7-3-6, #7-2-13) selected by brown rice Cd concentration as an index and Koshihikari without heavy ion beam treatment (Hereinafter simply referred to as Koshihikari.) Seeds were treated with Cd on the seedlings after 10 days in the same manner as in Example 1 by the hydroponics method. Four days after the Cd treatment, the harvest was carried out, and the leaves and roots were divided into stems and leaves and dried. This dried material was decomposed with strong acid by the same method as the brown rice of Example 3. In addition to Cd, the decomposed solution contains manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn), which are essential elements for plants, and all these elements are passed through inductively coupled plasma. Simultaneous measurement was performed with an optical emission analyzer (ICP-OES) (manufactured by Agilent Corporation, ...

Embodiment 3

[0143]

[0144] (Microarray Chip Experiment)

[0145] Using the mutant (#3-6-4) and Koshihikari root, RNA was extracted according to the protocol of RNA Purification Kit (manufactured by Rizo Co., Ltd., trade name: RNA Sui Sui-S). The concentration of RNA was detected by a spectrophotometer (manufactured by Thermo Fisher Scientific, trade name: NanoDrop NanoDrop 1000) to detect whether the extracted RNA was decomposed, and its quality was detected by Bio Analyzer 2100 from Agilent.

[0146] Using all the extracted RNA (400 ng), a reverse transcription reaction was performed using T7 promoter primer (Agilent) to synthesize cDNA. Labeling was synthesized by adding Cyanine3 (hereinafter referred to as Cy3) and Cyanine5 (hereinafter referred to as Cy5) for fluorescent labeling, and reacting in a Transcription Mix solution (Agilent) containing T7 RNA polymerase cRNA.

[0147] The labeled cRNA extracted and synthesized from each of the above individuals was purified using the Qi...

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Abstract

Provided are: a transporter gene involved in the promotion or inhibition of the absorption of Cd by a root; a mutant gene of the transporter gene; a transporter protein of the transporter gene; a rice plant of which the absorption of Cd is reduced; and a method for selecting and growing a rice plant of which the absorption of Cd is reduced. A gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 2; a gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 3; a gene encoding a transporter protein involved in the regulation of the absorption of cadmium, which comprises a DNA nucleotide sequence represented by SEQ ID NO: 4; and a transporter protein involved in the regulation of the absorption of cadmium, which comprises an amino acid sequence represented by SEQ ID NO: 1.

Description

technical field [0001] The present invention relates to a transporter gene and transporter involved in suppression of cadmium (hereinafter abbreviated as Cd.) uptake in roots, a rice mutant and a rice variety with low Cd uptake suppressed in Cd uptake. Background technique [0002] The FAO / WHO Contract Food Specification Committee has established international reference values ​​for the concentration of Cd in food in order to reduce the risk of health damage caused by the intake of Cd contained in food. Accepting this benchmark value, Japan revised the Food Sanitation Law in February 2011, and the specified value of rice was changed from 1mgkg -1 (Brown rice) largely changed to 0.4mgkg -1 (brown rice and polished rice). Therefore, it is urgent to develop techniques for reducing Cd uptake by rice. [0003] As techniques for reducing Cd uptake by rice, agricultural techniques such as soil restoration by soil replacement, input of soil improvement materials, and water storag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A01H1/00A01H1/02A01H5/00C07K14/415C12N5/10G01N33/15G01N33/50
CPCC07K14/415C12N15/8243
Inventor 石川觉仓俣正人安部匡井仓将人中西启仁西泽直子
Owner NAT INST FOR AGRO ENVIRONMENTAL SCI
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