38 results about "Drug transporter" patented technology

The invention discloses an application of a drug transporter YnfA in increasing the yield of cytidine, and belongs to the technical field of genetic engineering. In order to solve the problems of high pollution and high cost of traditional chemical synthesis of cytidine, the coding gene of the drug transporter protein YnfA is constructed to a recombinant plasmid pHX05, and an escherichia coli engineering strain SHX68 improved by a metabolic pathway is converted to obtain a recombinant strain SHX68 / pHX05. The strain promotes extracellular secretion of cytidine through overexpression of YnfA and relieves intracellular regulation inhibition. The shake-flask fermentation yield reaches 3.08 g / L and is increased by 33% compared with that of a contrast; the yield of 72h fermentation in a 5L fermentation tank is stabilized at 39g / L, which is obviously higher than 26g / L of a control strain. Chemical synthesis is replaced by biological fermentation, pollution and cost are reduced, and the requirement for large-scale industrial production of cytidine is met.