Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano gene drug transporter

A gene drug and carrier technology, applied in the field of spherical calcium phosphate nano-gene drug delivery carrier, can solve the problems of low shape and external load

Pending Publication Date: 2021-10-22
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the defects of calcium phosphate nanoparticles, such as shape and low external loading capacity, the application of calcium phosphate alone as a gene drug carrier is seldom used.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano gene drug transporter
  • Nano gene drug transporter

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] 1) Preparation of green fluorescent carbon quantum dots: Cysteine ​​and sucrose in equimolar ratio were added to a round bottom flask, and 20 mL of 0.1 mol / L sodium hydroxide solution was added. Stir and reflux at 80° C. for 24 hours to obtain an orange-yellow liquid. After cooling to room temperature, centrifuge to remove larger particles (11,000 rpm, 15 minutes), and collect the supernatant solution for later use. The particle size of carbon quantum dots is 1-9nm.

[0025] 2) Prepare a phosphate buffer solution with a pH value of 11; prepare a calcium chloride solution according to a calcium-phosphorus ratio of 1.7. Prepare 2mmol / L cetyltrimethylammonium bromide solution.

[0026] 3) Preparation of spherical calcium phosphate nanoparticles: add 2 mL of the above-prepared carbon quantum dot solution, 2 mL of cetyltrimethylammonium bromide solution, and 15 mL of distilled water to prepare a mixed solution in a round bottom flask, heat and stir in a water bath at 80°C ...

example 2

[0028] 1) Preparation of nucleic acid crystal nucleus: use protamine sulfate solution to polycondense nucleic acid into a spherical shape;

[0029] 2) Prepare a phosphate buffer solution with a pH value of 9; prepare a calcium nitrate solution according to a calcium-phosphorus ratio of 1.7. Prepare 2mmol / L tetradecyltrimethylammonium bromide solution.

[0030] 3) Preparation of spherical calcium phosphate nanoparticles: Add 0.5mL of the above-prepared nucleic acid solution, 0.5mL of tetradecyltrimethylammonium bromide solution, and 4mL of distilled water to prepare a mixed solution in a sterile round-bottomed flask, and place in a water bath at 40°C Heat and stir for 20min. Afterwards, 2 mL of phosphate buffer solution was added to the reaction system, and after heating and stirring for 20 min, 2 mL of calcium nitrate solution was added to the system, followed by condensation and reflux for 24 h. After the synthesis reaction is completed, the resulting suspension is washed w...

example 3

[0032] 1) Preparation of cisplatin-polylactic acid crystal nucleus: polylactic acid is used to wrap cisplatin in the way of water-in-oil-in-water, and the pellets of about 20 nm are made.

[0033] 2) Prepare a phosphate buffer solution with a pH value of 10; prepare a calcium nitrate solution according to a calcium-phosphorus ratio of 1.7. Prepare 2mmol / L octadecyltrimethylammonium bromide solution.

[0034] 3) Preparation of spherical calcium phosphate nanoparticles: suspend the above-mentioned cisplatin-polylactic acid nanospheres in 0.5 mL aqueous solution, add 0.5 mL of octadecyltrimethylammonium bromide solution, and 4 mL of distilled water to prepare a mixed solution in the absence of In a round-bottomed flask, heat and stir in a water bath at 50°C for 20 min. Afterwards, 2 mL of phosphate buffer solution was added to the reaction system, and after heating and stirring for 20 min, 2 mL of calcium nitrate solution was added to the system, followed by condensation and ref...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
sizeaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a nano gene drug transporter, in particular to a nano gene drug transporter which has biodegradability and good biocompatibility, has a unique loading mode, carries unique fluorescence performance and can be used for microimaging technology and visibility research. According to the invention, a preparation method of growth type spherical calcium phosphate on a crystal nucleus is firstly designed, and then carbon quantum dots are introduced as the crystal nucleus, so that the obtained carrier obtains fluorescence performance; and the spherical calcium phosphate nano gene carrier drug carrier is prepared by taking the gene and the drug as crystal nucleuses. In the synthesized calcium phosphate nano gene drug carrier, the crystal nucleus comprises quantum dots, carbon quantum dots, drugs, nucleic acid and the like; the gene or the drug can serve as a crystal nucleus and can be loaded on the crystal nucleus or outside the spherical calcium phosphate, and loading modes comprise physical adsorption, covalent interaction, ionic bond interaction and the like.

Description

technical field [0001] The invention relates to a spherical nanometer gene drug delivery carrier, in particular to a spherical calcium phosphate nanometer gene drug delivery carrier with biodegradability and good biocompatibility. Background technique [0002] Non-viral gene drug carrier, that is, a carrier system that uses carriers other than viruses to achieve gene and drug transfer. It can overcome the defects of small viral vector capacity, complex preparation process, and lack of tumor tissue targeting, and has the advantages of low immune response, easy synthesis, and no risk of pathogenicity, so it is widely used. Due to the unique vascular structure of tumor tissue, nanoparticles tend to accumulate in tumor tissue, so nano-gene drug carriers are widely used in anti-tumor therapy. [0003] When designing nanogene drug carriers, long-standing challenges that need to be overcome are the biocompatibility and biodegradability of the carrier. [0004] Hydroxyapatite, whi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/02A61K48/00A61P35/00A61P29/00C01B25/32C01B32/15B82Y40/00B82Y30/00
CPCA61K9/1611A61K48/0025A61P35/00A61P29/00C01B25/325C01B32/15B82Y40/00B82Y30/00C01P2004/32C01P2004/80
Inventor 孙艳叶凌涛刘献斌李育霖王羽张赫
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com