The invention discloses an
agrobacterium rhizogenes-mediated one-step transformation method for hairy roots of
caragana intermediary. The method comprises the following steps: S1, electrically transforming plasmids containing e-GFP expression vectors into
agrobacterium rhizogenes K599 competent cells; S2, preparing an
agrobacterium rhizogenes bacterial suspension with the OD600nm of 0.8 to 0.95, and adding
acetosyringone into the agrobacterium rhizogenes bacterial suspension; S3, culturing bacterial paste for infection; and S4, selecting a
caragana intermedia
seedling, obliquely
cutting a part which is 1cm below a cotyledonary node of the
seedling, discarding the lower part, infecting a
cut, then
transplanting the
seedling back into soil, pouring a 1 / 4B5 culture medium to the seedling, and putting the seedling into an illumination
incubator for culturing. According to the method, the
caragana intermediary hairy roots are converted through a one-
step method, so that the genes are over-expressed in the hairy roots, and compared with a
tissue culture method for converting the caragana intermediary hairy roots, the method is simpler to operate, does not need to prepare sterile seedlings, and is shorter in detection time, high in
gene expression level and convenient to detect.