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35results about How to "Increased gene expression levels" patented technology

ACETYL CoA CARBOXYLASE (ACCase) GENE FROM JATROPHA CURAS

The present invention provides methods for increasing oil content in Jatropha Curcas L. plants. The invention further provides the cDNA and protein sequences of Jatropha acetyl CoA carboxylase (ACCase) and methods for cloning and expressing the Jatropha ACCase gene to produce transgenic plants with increased oil content.
Owner:RELIANCE LIFE SCI PVT

Optimization of transgene expression in mammalian cells

InactiveUS20060051331A1Increase capacityIncrease transgene expressionSenses disorderFungiTransgeneMammalian cell
The present invention relates to vectors, compositions and methods for delivering transgenes into mammalian cells. The invention also relates to genetic constructs and recombinant cells suitable to produce such transgenes. The invention more particularly relates to a vector suitable for transgene delivery into mammalian cells, wherein said vector comprises a chimeric genetic construct comprising a transgene operably linked to at least two distinct posttranscriptional regulatory elements functional in mammalian cells. This invention can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.
Owner:CENT NAT DE LA RECHERCHE SCI +1

WT1 antisense oligos for the inhibition of breast cancer

The present invention provides methods for inhibiting the growth of breast cancer cells and methods for treating breast cancers expressing Wilms' Tumor 1 (WT1) gene product using a WT1 antisense oligonucleotide. It further provides methods of predicting breast cancer progression and methods for the screening of candidate substances for activity against breast cancer.
Owner:BOARD OF RGT THE UNIV OF TEXAS SYST

Dual-promoter inducible secretable shuttle plasmid and construction method thereof

The invention belongs to the field of biological genetic engineering, and provides a dual-promoter inducible secretable shuttle plasmid. The shuttle plasmid consists of an enzyme cutting Escherichia coli pET-28a plasmid and an enzyme cutting bacillus subtilis pE194 plasmid, wherein a lysozyme-antibacterial peptide functional fragment and an antibacterial peptide gene are inserted into the enzyme cutting Escherichia coli pET-28a plasmid. The invention also provides a method for constructing the dual-promoter inducible secretable shuttle plasmid. The shuttle plasmid is transferred to a prokaryote to express proteins such as toxalbumin, cecropin and the like in a fusion mode without toxicity to a host. The shuttle plasmid constructed by the method can be induced by IPTG or lactose to improve gene expression level; the shuttle plasmid is also suitable for exocrine expression, and a target protein for the exocrine expression is convenient to process and is not polluted by endotoxin; and because the shuttle plasmid has dual functional promoters, different genes can be inserted in appropriate correct directions for gene expression.
Owner:FUDAN UNIV

Solution and method for culturing bovine somatic cell cloned embryos

The invention provides a solution and method for culturing bovine somatic cell cloned embryos. The culture solution comprises merchant oviduct culture solution for bovine somatic cell cloned embryos (mSOF), and comprises activin A as well, wherein the concentration of the activin A is 20 to 80 ng / mL. The culture method comprises: culturing a bovine somatic-cell granular cell monolayer under conventional conditions for 3 days; adding the activin A in a concentration of 20 to 80 ng / mL to the merchant oviduct culture solution for bovine somatic cell cloned embryos (mSOF) from the fourth day; obtaining the solution for culturing bovine somatic cell cloned embryos; and using the solution for culturing bovine somatic cell cloned embryos for 5 days to obtain the bovine somatic cell cloned embryos. The solution and the method have the advantages of improving the 8 / 16 cell rate, blastula rate and hatchability of embryos and significantly raising the gene expression level of Na / K-ATPase, Glut-1, ActR II, Smad2 and the like, and are particularly suitable for application in the field of bovine somatic cell cloning.
Owner:NORTHWEST A & F UNIV

Polyurethane/decellularized fibrous ring matrix fibrous scaffold and preparation and application thereof

The invention discloses a polyurethane / decellularized fibrous ring matrix fibrous scaffold, which has a core-shell structure. The polyurethane is used as a core and the decellularized fibrous ring matrix is used as a shell. Besides, the invention also discloses a preparation method of the polyurethane / acellular fibrous ring matrix fibrous scaffold. Polyurethane is used as a solute of an internal solution and a decellularized fibrous ring matrix is used as a solute of an external solution; coaxial electrostatic spinning is performed to obtain a polyurethane / acellular fibrous ring matrix fibrousscaffold. During the coaxial electrostatic spinning process, the flow velocity of the internal solution is 0.4 to 0.6 mL / h, the flow velocity of the external solution is 0.15 to 0.25 mL / h, the voltage of the mandrel is 15 to 17 kV, and the rotating speed of the mandrel is 180 to 220 rpm. In addition, the invention also discloses application of the polyurethane / acellular fibrous ring matrix fibrous scaffold in preparation of drugs for treating intervertebral disc degenerative diseases.
Owner:THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE

Application of oxidized high-density lipoprotein

The invention belongs to the field of medicine, and particularly relates to an application of oxidized high-density lipoprotein. The oxidized high-density lipoprotein is used as a target or a marker of calcified aortic valve diseases. The oxidized high-density lipoprotein and / or an oxidized high-density lipoprotein detection reagent can be used for preparing a diagnostic agent of the calcified aortic valve diseases, monitoring a concentration of the oxidized high-density lipoprotein and predicting occurrence and a progress of the calcified aortic valve diseases. An inhibitor or blocker of theoxidized high-density lipoprotein can also be used for preparing medicine for treating the calcified aortic valve diseases and treating the calcified aortic valve diseases. The oxidized high-density lipoprotein and / or the oxidized high-density lipoprotein detection reagent can also be used for preparing a screening agent of calcified aortic valve disease treatment medicine and screening the medicine for treating the calcified aortic valve diseases. The application of the oxidized high-density lipoprotein provides a new way and basis for clinical diagnosis and treatment of the calcified aorticvalve diseases.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Adenovirus vector carrying inducible co-stimulater gene, and construction method and application of adenovirus vector

The invention relates to the field of biotechnology. Hematopoietic stem cell transplantation is a unique way of curing malignant hematologic diseases, but the complications in the hematopoietic stem cell transplantation process are still key factors causing death of the patient, and the most prominent is graft-versus-host disease (FVHD). How to prevent and treat the GVHD is a severe challenge to hematopoietic stem cell transplantation. The invention provides an adenovirus vector carrying inducible co-stimulater (ICOS) gene, and a construction method for the adenovirus vector. After the adenovirus vector transfects mesenchymal stem cells (MSCs), an aim of preventing and treating the GVHD is fulfilled by a method of combining the effect of the ICOS in the T lymphocyte activating process and the effect of the MSCs in the immune adjusting process.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY

Method for improving adaptive capacity of soybeans in saline-alkali soil

The invention discloses a method for improving the adaptive capacity of soybeans in saline-alkali soil. The method includes the steps that nutrient soil and perlite are mixed evenly to serve as bacterial manure matrixes, and then nodule bacteria fluid and the bacterial manure matrixes are mixed and stirred evenly to serve as nodule bacteria manure; soybean seedlings are transplanted into the nodule bacteria manure and are cultivated under the condition with the temperature being 23 DEG C to 27 DEG C, the illumination intensity being 2,000 lx to 2,500 lx and the illumination time being 12 h / d. The method utilizes the nodule bacteria manure to promote the whole plant fresh weight of the soybeans to be increased by from 22.50% to 125% in the soil with high-concentration salt, remarkably improves the salt tolerance related-gene expression level of the soybeans, and improves the mRNA expression level of peroxidases like APX and SOD by three times to eight times.
Owner:HANGZHOU NORMAL UNIVERSITY

Compositions and Methods for Diagnosing and Assessing Inflammatory Myopathies

The present invention is directed to assay methods for inflammatory myopathies and microarray plates that can be used in carrying out these assays.
Owner:THE BRIGHAM & WOMEN S HOSPITAL INC

Enhanced gene expression

The present disclosure is directed to a novel, unexpected approach of expressing exogenous gene(s) at increased levels by predictably, optionally irreversibly, incorporating the gene(s) into a region of increased gene expression (RIDGE) on a chromosome of a host cell. This approach is accomplished by identification of RIDGE(s) and further by integration of integrase-specific sites (e.g., attP or attB) in the presence of or mediated by integrase. The approach renders a high level of gene expression; predictability of the location of the exogenous genes, elimination of genetic instability or unwanted phenotype; and reduction of time and cost in optimizing protein production in host cells, which will be every useful in the production of therapeutic, prophylactic, and diagnostic proteins.
Owner:ASC THERAPEUTICS INC

Method of ameliorating chronic obstructive pulmonary disease using Parabacteroides goldsteinii

The present invention provides a method of improving the weight loss, the emphysema, the infiltration of inflammatory cells in lung tissues, the thickening of the tracheal wall, the lung fibrosis, and the abnormal lung function, which are caused by a chronic obstructive pulmonary disease, comprising administering to a subject in need thereof a therapeutically effective amount of a probiotic bacterium Parabacteroides goldsteinii. The bacterium Parabacteroides goldsteinii can also modulate the expression level of genes involved in the lung fibrosis and the mitochondrial activity in lung tissue. The bacterium Parabacteroides goldsteinii can therefore be used to treat chronic obstructive pulmonary disease.
Owner:MULTISTARS BIOTECHNOLOGY COMPANY LIMITED

Agrobacterium rhizogenes-mediated one-step transformation method for hairy roots of caragana intermediary

The invention discloses an agrobacterium rhizogenes-mediated one-step transformation method for hairy roots of caragana intermediary. The method comprises the following steps: S1, electrically transforming plasmids containing e-GFP expression vectors into agrobacterium rhizogenes K599 competent cells; S2, preparing an agrobacterium rhizogenes bacterial suspension with the OD600nm of 0.8 to 0.95, and adding acetosyringone into the agrobacterium rhizogenes bacterial suspension; S3, culturing bacterial paste for infection; and S4, selecting a caragana intermedia seedling, obliquely cutting a part which is 1cm below a cotyledonary node of the seedling, discarding the lower part, infecting a cut, then transplanting the seedling back into soil, pouring a 1 / 4B5 culture medium to the seedling, and putting the seedling into an illumination incubator for culturing. According to the method, the caragana intermediary hairy roots are converted through a one-step method, so that the genes are over-expressed in the hairy roots, and compared with a tissue culture method for converting the caragana intermediary hairy roots, the method is simpler to operate, does not need to prepare sterile seedlings, and is shorter in detection time, high in gene expression level and convenient to detect.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Poxvirus-derived promoter, and vector comprising same

The present invention provides a poxvirus-derived promoter, a vector comprising the same, a method for expressing a transgene using the promoter, and use of the vector in the prevention or treatment of a disease. A promoter according to the present invention can be used for induction of strong expression of a transgene.
Owner:KOLON LIFE SCI

Constitutive promoter and application thereof

The invention relates to a constitutive promoter and application thereof. The nucleotide sequence of the constitutive promoter comprises the sequence of (a) or (b): (a) the nucleotide sequence as shown in SEQ (sequence) ID (identity) NO: 1; (b) the nucleotide sequence which is hybridized with the nucleotide sequence limited by (a) under strict conditions. The constitutive promoter provided by the invention almost generally has activity in most of tissues and many types of cells of plants, and can significantly improve the expression level of a target heterologous nucleotide sequence in the tissues of the plants, in particular in leaf blades and stems.
Owner:BEIJING DABEINONG BIOTECHNOLOGY CO LTD

Intestinal mucosa protective agent applied to penaeus vannamei

The invention provides an intestinal mucosa protective agent applied to penaeus vannamei. The intestinal mucosa protective agent is prepared from the following components in parts by weight: 15-20 parts of glutamine dipeptide, 10-15 parts of arginine, 5-10 parts of inositol, 10-15 parts of radix puerariae, 10-15 parts of radix astragali seu hedysari, 15-20 parts of chitosan oligosaccharide, 5-10 parts of radix salviae miltiorrhizae and 10-15 parts of vitamin C phosphate. All the components of the health-care agent are crushed to 200 meshes and are uniformly mixed. In a feed processing process,the intestinal mucosa protective agent is used as an additive and is added according to the ratio of 0.1 percent to 0.2 percent to prepare functional feed. In a culture production process, the intestinal mucosa protective agent is fed for one period every 10 days from the last ten-day period of June to the last ten-day period of August every year. The intestinal mucosa protective agent is obtained from scientific experiment results and has scientific compatibility; an operation method is simple and convenient. The protective agent has a remarkable protection effect on intestinal mucosa of thepenaeus vannamei and can be used for remarkably reducing the occurrence rate of jejunum and empty stomach and white stool diseases of the penaeus vannamei and remarkably improving the culture economic benefits of the penaeus vannamei.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

A kind of preparation method and application of bifidobacterium monobacteria fermented milk

The invention discloses a method for preparing bifidobacterium single-bacteria fermented milk and an application thereof, belonging to the field of dairy product processing. The sticky exopolysaccharide-producing bifidobacterium strain used in the present invention is Bifidobacterium longum subsp. longum YS108R strain. The bifidobacterium fermented milk contains ≥10 8 CFU / g of live bifidobacteria, exopolysaccharide content of 130mg / L. Compared with products produced by fermentation of commercial starters, the bifidobacterium fermented milk of the present invention has a lower whey precipitation rate and higher water holding capacity, and has a sticky, delicate taste. The bifidobacterium fermented milk of the present invention also has the effect of alleviating colitis.
Owner:JIANGNAN UNIV

Bacillus for prawn feed and fermented Chinese herbal medicine preparation for prawn feed

The invention discloses bacillus for a prawn feed and a fermented Chinese herbal medicine preparation for the prawn feed. The fermented Chinese herbal medicine preparation is prepared by the following steps of crushing rehmannia glutinosa, angelica sinensis, white paeony roots and ligusticum wallichii, mixing according to a ratio, inoculating 3%-11% bacillus safensis and isothermal layer bacillus mixed with (1:1-1:4) bacterial liquid, and fermenting under proper conditions. The traditional Chinese medicine component is prepared from, by weight, 12-20 parts of rehmannia glutinosa, 9-25 parts of angelica sinensis, 9-12 parts of white paeony roots and 6-10 parts of ligusticum wallichii. The preparation method is simple and convenient, can increase the effective content of Chinese herbal medicines, and improves the utilization efficiency of the Chinese herbal medicines. When the bacillus is applied to the prawn feed, the immunity of prawns can be enhanced, the capability of the prawns for resisting pathogenic bacteria can be enhanced, the application of antibiotics and chemical drugs is reduced, the culture income is increased on the premises of green, efficiency and health, and a good application prospect is achieved.
Owner:OCEAN UNIV OF CHINA

Method of ameliorating chronic obstructive pulmonary disease using parabacteroides goldsteinii

The present invention provides a method of improving the weight loss, the emphysema, the infiltration of inflammatory cells in lung tissues, the thickening of the tracheal wall, the lung fibrosis, and the abnormal lung function, which are caused by a chronic obstructive pulmonary disease, comprising administering to a subject in need thereof a therapeutically effective amount of a probiotic bacterium Parabacteroides goldsteinii. The bacterium Parabacteroides goldsteinii can also modulate the expression level of genes involved in the lung fibrosis and the mitochondrial activity in lung tissue. The bacterium Parabacteroides goldsteinii can therefore be used to treat chronic obstructive pulmonary disease.
Owner:MULTISTARS BIOTECHNOLOGY COMPANY LIMITED

Constitutive promoter and application thereof

The invention relates to a constitutive promoter and application thereof. The nucleotide sequence of the constitutive promoter comprises the following sequence (a) or (b): (a) nucleotide sequence disclosed as SEQ ID NO:1; or (b) nucleotide sequence crossbred with the nucleotide sequence defined in (a) under strict conditions. The constitutive promoter generally has activity in most tissues and many types of cells of plants, and obviously enhances the expression level of the target heterologous nucleotide sequence in plant tissues especially in leaves and stems.
Owner:BEIJING DABEINONG BIOTECHNOLOGY CO LTD

Constitutive promoter and application thereof

The invention relates to a constitutive promoter and application thereof. The nucleotide sequence of the constitutive promoter comprises the following sequence (a) or (b): (a) nucleotide sequence disclosed as SEQ ID NO:1; or (b) nucleotide sequence crossbred with the nucleotide sequence defined in (a) under strict conditions. The constitutive promoter generally has activity in most tissues and many types of cells of plants, and obviously enhances the expression level of the target heterologous nucleotide sequence in plant tissues especially in leaves and stems.
Owner:BEIJING DABEINONG BIOTECHNOLOGY CO LTD

Phaeodactylum tricomutum diacylglycerol acyltransferase and encoding gene and application thereof

The invention discloses Phaeodactylum tricomutum diacylglycerol acyltransferase, an encoding gene and application thereof, and belongs to the technical field of genetic engineering. An amino acid residue sequence of the Phaeodactylum tricomutum diacylglycerol acyltransferase is shown as SEQ ID No: 2. The gene for encoding the Phaeodactylum tricomutum diacylglycerol acyltransferase is one of the following nucleotide sequences: 1, SEQ ID No: 1 in a sequence table, 2, a nucleotide sequence for encoding a protein sequence shown as SEQ ID No: 2 in the sequence table, and 3, a deoxyribonucleic acid(DNA) sequence which has more than 90 percent of homology with a nucleotide sequence defined in the SEQ ID No: 1 in the sequence table and encodes the same functional protein. Through the experiment,the transgenosis can remarkably improve the content of grease in algae, so that a high-quality gene is provided for improving the content of the grease in the algae; and the Phaeodactylum tricomutum diacylglycerol acyltransferase has broad application prospect and important application value.
Owner:SICHUAN UNIV
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