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Method for improving adaptive capacity of soybeans in saline-alkali soil

A technology of adaptability and saline-alkali land, applied in the direction of fertilization methods, botanical equipment and methods, applications, etc., to achieve the effect of improving expression levels and promoting expression levels

Active Publication Date: 2015-05-13
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a rhizobia fertilizer and its application to improve the adaptability of soybeans in saline-alkali land, and solve the problem of soybean adaptability in saline-alkali land

Method used

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  • Method for improving adaptive capacity of soybeans in saline-alkali soil
  • Method for improving adaptive capacity of soybeans in saline-alkali soil
  • Method for improving adaptive capacity of soybeans in saline-alkali soil

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 Rhizobium bacterial liquid

[0024] (1) Inoculate the rhizobia colony in TY / Ca 2+ In the culture medium, incubate at 30°C, 225rpm shaker for 12 hours to obtain mother liquor; TY / Ca 2+ Culture medium composition: 5g / L tryptone, 3g / L yeast extract, 6mmol / L CaCl 2 , pH=7.2, the solvent is water.

[0025] (2) Inoculate the seed solution of step (1) to TY / Ca with an inoculum volume concentration of 0.1% 2+ Proliferate in culture medium at 30°C, 225rpm shaker for 12 hours until OD 600 When the value reaches 1.0, a rhizobia bacterial liquid is obtained.

Embodiment 2

[0027] 1) Take 50 salt-tolerant soybeans of the Wenfeng07 variety with full particles and uniform particle size, soak them in Liby bleach for 5 minutes, and rinse them with tap water for 5 minutes. Then disinfect with 75% ethanol aqueous solution for 5 minutes, rinse with double distilled water for 3 times, disinfect with 0.1% mercury liter for 10 minutes, and then wash with sterile water for 5 times until the residual mercury liter is cleaned. absorbent paper to absorb the water on the surface of the seeds, which is the sterilized seeds;

[0028] 2) Place the sterilized seeds on a petri dish lined with moist sterile filter paper, and cultivate them in the dark at 37°C for 2 to 3 days until the seeds germinate, the two cotyledons open, and the buds are 2 to 3 cm long to obtain soybeans seedling;

[0029] 3) the rhizobia bacterium liquid prepared by embodiment 1 is diluted to OD with purified water 600 is 0.001, soak the soybean seedlings obtained in step 2) in the diluted Rh...

Embodiment 3

[0038] The specific steps are the same as in Example 2, except that the soybean variety adopts the main planted salt-sensitive variety Union 85140, and other operations are the same as in Example 2. The results are shown in image 3 As shown, the results showed that the salt tolerance of plants added with rhizobia fertilizer was significantly enhanced.

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Abstract

The invention discloses a method for improving the adaptive capacity of soybeans in saline-alkali soil. The method includes the steps that nutrient soil and perlite are mixed evenly to serve as bacterial manure matrixes, and then nodule bacteria fluid and the bacterial manure matrixes are mixed and stirred evenly to serve as nodule bacteria manure; soybean seedlings are transplanted into the nodule bacteria manure and are cultivated under the condition with the temperature being 23 DEG C to 27 DEG C, the illumination intensity being 2,000 lx to 2,500 lx and the illumination time being 12 h / d. The method utilizes the nodule bacteria manure to promote the whole plant fresh weight of the soybeans to be increased by from 22.50% to 125% in the soil with high-concentration salt, remarkably improves the salt tolerance related-gene expression level of the soybeans, and improves the mRNA expression level of peroxidases like APX and SOD by three times to eight times.

Description

(1) Technical field [0001] The invention relates to a method for growing soybeans in saline-alkali land, in particular to a method for promoting the growth of soybeans in saline-alkali land by utilizing rhizobia fertilizer. (2) Background technology [0002] my country is the birthplace of soybean cultivation, with a cultivation history of more than 3,000 years. In recent years, the problem of soil salinization has increasingly threatened the limited land resources that human beings rely on for survival. However, with the development of the national economy and the improvement of people's living standards, as well as the development of processing industry, food industry and animal husbandry, my country's demand for vegetable protein has increased sharply. Soybeans from the United States and South America make up. Insufficient soybean production has become an important obstacle to the national policy of "basically relying on domestic food supply", and has also become a new w...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C21/00C05F11/08A01C1/02A01C1/08
CPCA01C1/08A01G22/00C05F11/08
Inventor 皮二旭渠立群黄盈盈刘聪杜立群
Owner HANGZHOU NORMAL UNIVERSITY
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