A kind of preparation method and application of bifidobacterium monobacteria fermented milk

A technology of bifidobacteria longum and fermented milk, applied in bifidobacteria, biochemical equipment and methods, bacteria used in food preparation, etc., can solve the problems of slow growth and acid production rate of bifidobacteria, unfavorable curdling, etc. , to achieve the effects of reducing expression, improving colitis, and reducing whey precipitation rate

Active Publication Date: 2020-02-11
JIANGNAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Generally, the growth and acid production rate of bifidobacteria in milk is slow, and it is not conducive to curdling, which may cause excessive milk clearing.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method and application of bifidobacterium monobacteria fermented milk
  • A kind of preparation method and application of bifidobacterium monobacteria fermented milk
  • A kind of preparation method and application of bifidobacterium monobacteria fermented milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the isolation method of bacterial strain

[0032] The method for isolating bifidobacteria, the main steps are as follows:

[0033]The collected feces of the long-lived elderly were used as samples, and after the samples were serially diluted, three appropriate serial dilutions were selected and carried out on the MRS plate added with 0.05% cysteine ​​hydrochloride and 100mg / L mupirocin. After coating, place the plate upside down in an anaerobic incubator at 37°C for 48 hours; pick colonies with a good wire-drawing effect to streak and purify, and then inoculate them into MRS liquid medium containing 0.05% cysteine ​​hydrochloride to anaerobically Incubate in oxygen for 24-48 hours, collect the fermentation broth for the determination of exopolysaccharides, and keep a small amount of fermentation broth, collect the bacteria by centrifugation, and use it for genome extraction, PCR and 16S rDNA sequencing. Determination of extracellular polysaccharides: take...

Embodiment 2

[0037] Example 2: Determination of the properties of bifidobacterium YS108R

[0038] Determination of biological characteristics of bifidobacteria:

[0039] (1) Observation method of colony characteristics: Streak the strain on the MRS solid medium, culture until a single colony grows, and observe the colony morphology.

[0040] Colony characteristics: Form round and raised colonies on MRS solid medium, milky white, opaque, smooth surface, colony diameter of 1-3 mm, with a drawing effect, as attached figure 1 (A) shown.

[0041] (2) Observation method for bacterial characteristics: pick a small amount of colonies and smear them on a glass slide, perform Gram staining, and observe under an optical microscope.

[0042] Thale characteristics: Gram staining is positive, grows well in the environment of pH 3.0-8.0, does not form spores, the bacterial body is about 0.5-1.5 μm×2-8 μm, and is rod-shaped, rod-shaped or bifurcated Y-shaped, optical Microscopic and transmission electr...

Embodiment 3

[0050] Example 3: Relief effect of bifidobacterium fermented milk on DSS-induced colitis mice symptoms

[0051] Prepare 40 male C57 mice and randomly divide them into 5 groups: normal control group (Control), model group (Model), YS108R fermented milk intervention group (YS108R-FM), BB12 fermented milk intervention group (BB12-FM group) and Lactobacillus bulgaricus + Streptococcus thermophilus fermented milk intervention group (SL-FM), 8 rats in each group. The experimental scheme and the treatment methods of the mice in each group are shown in Table 5, and the intragastric administration dose of each mouse was 0.2 mL. On the 14th day, dextran sodium sulfate (DSS) was made into a solution with a concentration of 2.5% with physiological saline, which was used for modeling by mice instead of drinking water, and was prepared and replaced every two days. During the DSS modeling period (15-21 days), the body weight, fecal status, and fecal occult blood of the mice were recorded ev...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for preparing bifidobacterium single-bacteria fermented milk and an application thereof, belonging to the field of dairy product processing. The sticky exopolysaccharide-producing bifidobacterium strain used in the present invention is Bifidobacterium longum subsp. longum YS108R strain. The bifidobacterium fermented milk contains ≥10 8 CFU / g of live bifidobacteria, exopolysaccharide content of 130mg / L. Compared with products produced by fermentation of commercial starters, the bifidobacterium fermented milk of the present invention has a lower whey precipitation rate and higher water holding capacity, and has a sticky, delicate taste. The bifidobacterium fermented milk of the present invention also has the effect of alleviating colitis.

Description

technical field [0001] The invention relates to a method for preparing bifidobacterium single-bacteria fermented milk and an application thereof, belonging to the field of dairy processing. Background technique [0002] Lactic acid bacteria have important applications in the fermented dairy industry, and the characteristics of the strains have a significant impact on the physicochemical properties of fermented dairy products. Lactic acid bacteria commonly used in the production of fermented milk are Streptococcus thermophilus and Lactobacillus bulgaricus. However, with the increasing research on the health effects of bifidobacteria and the discovery of potential commercial benefits, there are more and more researches on bifidobacteria fermented milk and the development of related products. Studies have shown that some bifidobacteria fermented milk has health functions such as improving blood lipid levels, relieving colitis, preventing diarrhea and constipation, and regulati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/127A23L33/135A61K35/745A61P1/00C12R1/01
CPCA61P1/00A23C9/127A23L33/135A23V2002/00A61K2035/115C12R2001/01C12N1/205A23V2400/533A23V2200/32
Inventor 杨波陈卫赵建新王刚闫爽张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap