The present invention provides a biological composite artificial trachea and its preparation method in combination with 3D printing technology. The material used in the present invention has both flexibility and certain
mechanical strength, which meets the physical and mechanical properties of the trachea as a hollow organ in the
thoracic cavity; secondly, The artificial trachea of the present invention has a porous and loose structure, which is conducive to the
diffusion of nutrients and the growth of
autologous blood vessels; in addition, the present invention prints the seed cells obtained from the expansion of the recipient's own
airway epithelial cells and chondrocytes in the trachea wall, The patient's rejection of the cells is avoided; moreover, the seed cells are composed of terminally differentiated mature cells, which avoids the problem of inducing differentiation and the risk of
tumor formation caused by the use of stem cells; at the same time, the
spatial distribution of the cells in the present invention is The printing is precise and controllable, and the distribution according to histological laws accelerates the repair process; finally, the
tracheal stent can be degraded in the body, reducing the complications caused by
foreign body residues in the body, and avoiding the risk of secondary
surgical removal.