Preparation method and application of a biological composite artificial trachea
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An artificial trachea and biological technology, applied in the field of medical devices, to avoid the risk of secondary surgery removal, avoid rejection, and avoid dependence
Inactive Publication Date: 2017-12-26
SHANGHAI PULMONARY HOSPITAL +1
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However, due to the limitations of current equipment development and the particularity of the structure and function of the trachea, there is currently no mature and practical trachea bioprinting solution.
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[0040] The invention provides a method for preparing a biological composite artificial trachea, comprising the following steps:
[0041] Step 1, using a bronchoscope, aseptically brush the bronchial epithelial cells, culture and expand in vitro; extract auricle chondrocytes, digest, disperse, and culture and expand in vitro;
[0042] Step 2, mix collagen and hyaluronic acid with deionized water, filter and sterilize through a bacteria filter to obtain soft materials; after fumigation of chitosan with ethanol, irradiate with ultraviolet light for complete sterilization, and evenly disperse in the sterile in culture medium;
[0043] Step 3, mixing the soft material obtained in step 2 with the culture medium at a volume ratio of 1:1 to make a soft material mixture for resuspending cells;
[0044] Step 4, dissolving the polylactic acid-glycolic acid copolymer and polyethylene glycol in dichloromethane, stirring ultrasonically to make the dispersion uniform, and placing it in a fu...
Embodiment 1
[0051] Using a bronchoscope, the bronchial epithelial cells were collected from sterile transplant recipients by brushing, cultured and expanded in vitro; auricular cartilage was extracted by surgery, digested and dispersed, and cultured and expanded in vitro.
[0052] Mix 10% collagen and 5% hyaluronic acid with deionized water according to mass-volume ratio, filter and sterilize through a bacteria filter to obtain a soft material. After the chitosan is fumigated with ethanol, it is irradiated under an ultraviolet lamp for 24 hours to completely sterilize it, and is dispersed in the culture solution at a mass volume ratio of 8%.
[0053] Mix the above two solutions 1:1 to make a soft material mixture for resuspending cells.
[0054] The two kinds of cells amplified in vitro were respectively digested, resuspended, counted and centrifuged, the supernatant was discarded, and resuspended in the aforementioned soft material mixture at a cell concentration of 1 million / ml to make ...
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Abstract
The present invention provides a biological composite artificial trachea and its preparation method in combination with 3D printing technology. The material used in the present invention has both flexibility and certain mechanical strength, which meets the physical and mechanical properties of the trachea as a hollow organ in the thoracic cavity; secondly, The artificial trachea of the present invention has a porous and loose structure, which is conducive to the diffusion of nutrients and the growth of autologous blood vessels; in addition, the present invention prints the seed cells obtained from the expansion of the recipient's own airway epithelial cells and chondrocytes in the trachea wall, The patient's rejection of the cells is avoided; moreover, the seed cells are composed of terminally differentiated mature cells, which avoids the problem of inducing differentiation and the risk of tumor formation caused by the use of stem cells; at the same time, the spatial distribution of the cells in the present invention is The printing is precise and controllable, and the distribution according to histological laws accelerates the repair process; finally, the tracheal stent can be degraded in the body, reducing the complications caused by foreign body residues in the body, and avoiding the risk of secondary surgical removal.
Description
technical field [0001] The invention relates to the field of medical devices, in particular to a preparation method and application of a biological composite artificial trachea. Background technique [0002] For malignant tumors of the trachea, benign space-occupying lesions, or tracheal stenosis, or trauma, after long-segment tracheectomy (more than 50% of the total length in adults, and more than 30% in children), the resection margins cannot be directly anastomosed, and a tracheal substitute is required. The anastomosis can be performed only if it is inserted, and the breathing and ventilation function of the trachea can be restored. According to Neville et al., an ideal trachea replacement material needs to have the following characteristics: (1) sealed lumen structure; (2) flexible texture; (3) good biocompatibility; (4) low inflammatory response ; (5) low immunity; (6) contribute to the growth of tracheal epithelial tube lining. With the deepening of researchers' res...
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